文献信息：刘霞,彭抒昂,郭文武等.  发表于【园艺学报】 |  , 请登录

简介：采用石蜡切片法和组织离析法分别对3种柑橘实生砧木和2种体细胞杂种的根系木质部和导管分子的解剖结构进行了比较。结果表明: (1)粗柠檬、红橘和枳3种实生砧木根系的木质部和韧皮部呈同心圆环状排列,而‘红橘+枳’和‘红橘+粗柠檬’两种体细胞杂种木质部和韧皮部呈"海星状"排列,结构异常。(2)导管所占的面积和密度均以生长势较强的粗柠檬为大,以生长势较弱的枳为小;体细胞杂种没有明显规律。(3)柑橘导管分子属于孔纹导管,穿孔板均为单...

文献信息：闫虎斌等.  请登录

简介： 柑桔衰退病毒是归类于长线性病毒科(Closteroviridae)长线性病毒属(Closterovirus)的一种正义链单分体RNA病毒，主要通过蚜虫和嫁接传播，长期以来对世界柑桔产业构成严重威胁。CTV主要危害以酸橙作砧本的大多数柑桔品种以及影响嫁接在其它砧本上的柚类、葡萄柚和部分甜橙的正常生长。 本实验是基于RNAi/PTGS亦即RNA沉默技术原理，用柑桔衰退病强毒系基因组部分RNA序列，构建表达后可形成200-400bp发卡形回文结构RNA(hpRNA)的反向重...

文献信息：许淼等.  请登录

简介：温度是影响植物生长和分布的重要因素。周期性寒害往往引起柑橘果实产量降低、品质下降，甚至树体死亡，从而造成巨大的经济损失。迄今为止，全球的柑橘冻害问题仍然没有得到有效解决。因此培育柑橘的抗寒品种成为柑橘业中一个急需解决的问题。随着植物分子生物学的发展，转基因已成为农作物育种新的技术，植物的抗寒性状是复杂的数量性状，是低温下多基因共同协作的结果。若采用单一的抗寒基因转化植物，虽然能一定程度上提高转基因...

文献信息：张家银等.  请登录

简介：柑橘在世界农业经济中占有重要地位，但其产业发展一直受到柑橘各种病害的制约。栽培措施与农药对柑橘病害的防治能力有限，且化学农药还会造成严重的环境污染，抗病育种成为解决柑橘病害的必然选择。柑橘常规育种受到育种周期长、珠心胚干扰、性器官败育、遗传上高度杂合等因素影响而进程缓慢，同时物种间生殖隔离亦阻碍了常规育种进程。转基因技术的应用与抗病相关基因的克隆为柑橘抗病育种提供了新的思路。本研究采用农杆菌介导法...

文献信息：刘利平等.  请登录

简介：柑橘是最重要的热带和亚热果树，产量位居世界水果之首。柑橘易遭受冻害和病虫害等自然与非自然因素影响，随着人们生活水平的提高和生活习惯的改变，柑橘的育种目标也在不断发生变化。由于柑橘的多胚性、杂交不亲合性、雄性或雌性不育性等障碍，常规育种途径很难满足定向培育新品种的要求。基因工程的应用为柑橘品种的改良开辟了一条新途径，而建立一个高效的、适宜于遗传转化的柑橘离体再生系统则是开展转基因工作的基础。 本研究...

文献信息：胡春华等.  请登录

简介：柑橘溃疡病(citrus bacterial canker disease)是由地毯黄单胞杆菌柑橘致病变种(Xanthomonas axonopodis pv.citri)引起的一种世界性检疫性病害，对柑橘产量及品质影响极大，严重危害着柑橘产业的健康发展。然而，到目前为止，尚无一种能根除柑橘溃疡病菌的最佳方法，柑橘品种资源中亦未发现完全抗病的品种，长期的常规育种，也没有获得抗柑橘溃疡病的优良杂种，柑橘抗溃疡病育种没有取得实质性进展。病原基因pthA是柑橘溃疡病亚洲型...

文献信息：仝铸等.  请登录

简介：柑橘是世界上最重要的水果之一，果实品质一直是柑橘育种追求的主要目标。长期以来，童期长、珠心胚、性器官败育以及遗传上的高度杂合等因素一直严重影响着柑橘育种工作的开展。基因工程的发展为柑橘的育种工作开辟了一条新的途径，从而使利用单个基因改造柑橘的品质成为可能。本研究利用童期较短的早实枳作为转化材料，建立并优化了其高效再生和遗传转化的体系，以期使其成为柑橘基因功能验证和研究的平台。利用该转化体系和RNA干涉...

文献信息：曾黎辉, 徐海峰, 王会全, 吴少华, 朱艺萱.等.  发表于【农业生物技术学报】 |  , 请登录

简介：The plant expression vectors pCAMBIA1301PMI and pBIPMI were constructed by substituting Escherichia coli phosphomannose-isomerase(PMI)gene for hpt gene of pCAMBIA1301 and gus gene of pBI121.Epicocyl explants of Xuegan sweet orange(Citrus sinensis L.Osbeck)were inoculated with Agrobacterium-tumefaciens EHA105-pCAMBIA1301PMI and EHA105-pBIPMI and subsequently selected on medium supplemented with a c...

文献信息：段艳欣, 李鼎立, 郭文武.等.  发表于【《2008园艺学进展（第八辑）——中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集》】 |  , 请登录

简介：Threre are significant differences in tansformation effieiency between different citrus embryogenic cailuses. Embryogenic calluses of 11 citrus genotypes ( including Citrus reticulata, C. sinensis，C. Paradisi, Fortunella crassifolia, etc), were assessed for Agrobacterium tumefaciens mediated transformation potentials. GUS positive callus lines from 10 genotypes were produced and the transformati...

文献信息：蔡小东.等.  发表于【长江大学学报(自然科学版)】 | 请登录

简介：Protoplast isolation,culture and embryoid induction were carried out for transgenic GFP(pBIN-mGFP5-ER)Guoqing No.1 embryogenic callus.All purified transgenic GFPCitrus unshiuMarc.cv.Guoqing No.1 protoplasts expressed strong GFP,and the viability reached about 87%.First cell division was observed about 12~14 d after protoplast cuture,and visible colonies were formed after 40 d of protoplast culture...

文献信息：Ballester A, Cervera M, Peña L.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：The neomycin phosphotransferase (nptII) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants. The present work investigates three different selection alternatives, comparing them to nptII selection in two citrus genotypes, Carrizo citrange...

文献信息：Silva RP, Mendes BMJ, Mourão Filho FAA.等.  发表于【Pesquisa Agropecuária Brasileira】 |  , 请登录

简介：The objective of this work was to evaluate the induction and formation of adventitious buds in sour orange explants through the use of plant regulators. In vitro organogenesis experiments were conducted to evaluate the effect of BAP, TDZ, and KIN in different concentrations and under two light conditions; BAP and KIN, combined or not with NAA; BAP and KIN, separately or in combined concentrations....

文献信息：罗赛男, 邓子牛, 钟晓红, 张家银, 袁飞荣, 杨莉.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：A parthenocarpy gene(DefH9-iaaM)was introduced into Succari to obtain possible seedless transformants, by using internodal stem segments as explants to establish an efficient regeneration and transgenic system.The results indicated that 30-day-old seedlings were the most efficient explants.The co-culture in darkness for 3~6 days gave a rise to good regeneration.The optimum medium for adventitious ...

文献信息：罗赛男, 邓子牛, 钟晓红, 易图永, 黄荣峰, 曾柏全.等.  发表于【中南林业科技大学学报】 |  , 请登录

简介：An experiment was conducted by inoculating citrus bacterial canker and citrus anthracnose to the leaves of transgenie Succari．Results show that TERFl gene can enhance the transgenic plants with their broad—spectrum disease resistance；that after an inoculation with citrus bacterial canker, their incidence rate is reduced by 50％，that compared tO Bingtang Orange and CK, transgenic Succari also d...

文献信息：罗赛男, 钟晓红, 邓子牛, 黄容峰.等.  发表于【江西农业大学学报】 |  , 请登录

简介：After establishing the regeneration and transformation systems of citrus, the TERF1 gene was mediated into ssweetorange byEHA105 and LB4404Agrobacterium tumefaciens. Seven plantswere verified by PCR and RT-PCR amplification. The results showed that EHA105 had more efficiency in improving the transgenic ratio than LBA440

文献信息：Batuman O, Mawassi M, Dawson WO, Bar Joseph M.等.  发表于【INDIAN JOURNAL OF VIROLOGY】 | 请登录

文献信息：Kunta M, Skaria M, Da Graca JV, Mirkov T, Louzada ES.等.  发表于【PHYTOPATHOLOGY】 | 请登录

文献信息：谢玉明, 郭琛, 张家银, 杨丽, 胡春华, 邓子牛.等.  发表于【分子植物育种】 |  , 请登录

简介：Genetic transformation systems from mature tissue of citrus have to be achieved as a necessary requiremerit for the introduction of useful genes into specific cultivars and the rapid evaluation of the genetically modifled characteristic．This research presents a protoeol for in vitro regeneration and genetic transformation of C. sinensis Osbesk cv Newhall using as explants intemodal segments from ...

文献信息：Sala Junior V, Celloto VR, Vieira LGE, Gonçalves JEG, Gonçalves RAC, Oliveira AJB.等.  发表于【Plant Systematics and Evolution】 | 请登录

简介：Floral nectar chemical composition of conventional and transgenic sweet orange trees expressing the antibacterial peptide sarcotoxin IA (Stx IA), which has been shown to efficiently inhibit in vitro growth of the bacteria responsible for citrus canker (Xanthomonas axonopodis pv. citri), is reported. The nectar contents of primary metabolites (sugars, amino acids, proteins, triacylglycerides) and o...

文献信息：谢玉明, 郭琛, 张家银, 韩健, 袁飞荣, 邓子牛.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：The phyB gene was transferred into the genome of Poncirus trifoliate in vitro with explants of epicotyl mediated by Agrobacterium．To诅l 27 strains with plant resistance plants were regenerated．The presence of specific targeted strips of phyB gene in the 5 regenerated plants Was confirmed by PCR analysis．RT-PCR analysis indicated the regular expression of transgene phyB in two transgenic clones，...