文献信息：黄家权等.  请登录

简介：柑橘是世界上最重要的果树之一，在我国水果产业中占据了重要的经济地位。然而，周期性的冻害严重影响了柑橘的产量和品质，这个问题的解决有赖于柑橘的抗寒育种和抗寒栽培。柑橘类植物的多胚性、遗传背景复杂、童期长、单性结实的特点限制了柑橘常规育种的发展。柑橘组织培养以及建立在此基础上的遗传转化为缩短育种周期、获得抗寒、优质柑橘种质资源提供了新的途径。 本实验以冷敏感的尤力克柠檬(Citrus limon (L.) Burm. f. cv. E...

文献信息：刘歆等.  请登录

简介：柑橘是世界第一大水果。通过遗传改良方法创造优质、多抗及不同成熟期的品种类型，是我国柑橘育种研究的重点。然而柑橘属植物存在珠心胚干扰、童期长、雄雌性败育等现象，传统育种手段效率不高、进程缓慢。近年来，不断完善的转基因技术为我们提供了一种更加快速直接的育种和品种改良手段。 本实验以柑橘愈伤组织和试管播种实生苗上胚轴为外植体，用根癌农杆菌介导法进行绿色荧光蛋白基因(gfp)的转化，获得14个品种的转基因材料，同...

文献信息：刘高平等.  请登录

简介：柑橘是世界上最重要的水果之一，在所有水果种类中面积和产量均居第一位，在人们日常生活中占有重要的地位。但是柑橘产业中还存在着很多问题，如病虫害及自然灾害危害严重，成熟期过于集中，品种结构不合理等。特别是我国加入WTO后，国外优良柑橘进入我国市场，使我国柑橘业受到巨大挑战，这就需要培育出更多优良柑橘品种来参与市场竞争。我国许多地方良种例如椪柑、沙田柚等品种种子较多，影响市场竞争力。改良这些品种，实现无核化...

文献信息：胡春华等.  请登录

简介：果树矮化栽培可降低生产成本，提高生产效益。果树的矮化途径有多种，但其中最有效的为矮化砧木的应用。柑橘目前还没有一个有效的矮化砧木可在生产中应用，因此，矮化砧木的选育一直是柑橘砧木育种的主要目标之一。枳橙[C.sinensis(L.)Osb×Poncirus trifoliate(L.)Raf.]是甜橙与枳的杂种，是世界各柑橘生产国广泛应用的砧木，继承了枳壳抗脚腐病、抗衰退病的特性，并具有耐旱、耐寒、耐贫瘠，对土壤适用性强、与大多数商用品种亲和...

文献信息：周春丽等.  请登录

简介：佛手，学名Citrus medica L. var. sarcodactylis，为芸香科柑桔属香椽的变种。它不仅是闻香赏果的花卉珍品，又是传统的名贵中药。我国佛手的主要栽培地区有广东、福建、四川、浙江等，其中浙江金华所产的佛手品质最为优良，为佛手中的上品。但其冬季不抗寒，造成冬季大部分佛手受冻死亡，严重影响佛手的品质和产量。随着现代生物技术的迅猛发展，基因工程为解决这一问题提供了有效的方法和途径。　本试验同时研究了农杆菌介导和基因...

文献信息：Fagoaga C, López C, Moreno P, Navarro L, Flores R, Peña L.等.  发表于【Molecular Plant-Microbe Interactions】 | 请登录

简介：Ectopic expression of the p23 gene from a severe (T36) strain of Citrus tristeza virus (CTV) induces viral-like symptoms in Mexican lime. Here, we report that expressing the same gene from a mild strain induced similar symptoms that correlated with accumulation of p23 protein irrespective of the source strain. CTV inoculation of transgenic limes showing CTV-like leaf symptoms and high p23 accumula...

文献信息：Kayim M, Koc NK.等.  发表于【Journal of Plant Biochemistry and Biotechnology】 | 请登录

文献信息：Ahmad M, Mirza B.等.  发表于【Plant Molecular Biology Reporter】 |  , 请登录

简介：In this study, conditions were optimized for transient gene expression in Rough Lemon (Citrus jambhiri Lush.), a major rootstock used in the citrus growing regions of Pakistan. Agrobacterium tumefaciens carrying the binary vector p35GUSINT, containing NPT 1I and GUS genes, was used in the study. The transformation method was based on injection of Agrobacterium into Citrus fruits followed by histoc...

文献信息：韩美丽, 吴耀军, 陆荣生, 杜晓莉.等.  发表于【广西林业科学】 |  , 请登录

文献信息：da Silva RP, Costa MAPD, Souza AD, de Almeida WAB.等.  发表于【PESQUISA AGROPECUARIA BRASILEIRA】 |  , 请登录

文献信息：谢玉明, 邓子牛, 郭琛, 焦徕, 胡春华, 熊兴耀.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：Seedling epicotyl (intemodal stem) segments of‘Xupu’sweet orange(Citrus sinensis L.0sb.) were used as expIants to establish Agrobacterium—mediated transfomation system with chit42 and phyB genes. The results showed that trallsformed buds could be obtained after the epicotyl segments were cultured on co-culture medium(MS+3 mg／L BA) for 3 days and then on selective medium with 100 mg／L Kanamyc...

文献信息：杨晓红, 孙中海, 仝瑞建.等.  发表于【中国农业科学】 | 请登录

简介：Genetic transformation experiments of the different exploids from Citrus grandis cv.Changshou Shatian You infected with Agrobacterium rhizogenes were carried out under dark and light.The optimized culture system for Ri T-DNA transformed roots of C.grandis cv.Changshou Shatian You was constructed as follows:After the ventral wounded striations on the single activation cotyledon were inoculated by A...

文献信息：Guo W, Duan Y, Olivares-Fuster O, Wu Z, Arias CR, Burns JK, Grosser JW.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：Valencia orange [Citrus sinensis (L.) Osbeck] is the leading commercial citrus species in the world for processed juice products; however, the presence of thermostable pectin methylesterase (TSPME) reduces its juice quality. A long-term strategy of this work is to eliminate or greatly reduce TSPME activity in Valencia orange. Previous work resulted in the isolation of a putative TSPME gene, CsPME4...

文献信息：Rosely Pereira da Silva, Maria Angélica Pereira de Carvalho Costa, Antonio da Silva Souza, Weliton Antonio Bastos de Almeida.等.  发表于【Pesquisa Agropecuária Brasileira】 |  , 请登录

简介：The objective of this work was to establish an efficient protocol for in vitro regeneration of plants through organogenesis of sweet orange ‘Pera’ (Citrus sinensis L. Osbeck) juvenile explants, in order to give support for future studies of genetic transformation. Epicotyl segments used as explants were introduced in MT culture media. To maximize the regeneration of in vitro plants, experiments ...

文献信息：周春丽, 郭卫东, 王德解, 余初浪, 路梅, 李玉萍.等.  发表于【西北植物学报】 |  , 请登录

简介：Bergamot is a plant of high medicinal and ornamental values that belongs toCitrus medicaL.var. sarcodactylis(Noot.) Swingle,but does not have many varieties.Thus it is necessary to conduct its genetic improvement and variety breeding.In order to apply particle bombardment to the genetic improvement and variety breeding of bergamot,this study,usingGUSgene as the report gene,transformedGUSgene into ...

文献信息：李东栋, 邓秀新.等.  发表于【分子植物育种】 |  , 请登录

简介：In this study,15 kinds of calluses from different citrus varieties were transformed following the developed system.The result showed that12 kinds of varieties'calluses among 15 could receive a certain number of resistant calluses, and 3 varieties' could not obtain any resistant calluses.Transgenic plants of 4 kinds of cultivars were first reported in this papersuch as Guoqing No.1,Hong Kong Kum qu...

文献信息：Cirvilleri, Spina S, Scuderi G, Gentile A, Catara A.等.  发表于【Journal of Plant Pathology】 | 请登录

简介：To study the influence of rolABC Troyer citrange plants on potentially beneficial root-associated bacteria, transgenic and non-transgenic plant lines were monitored over the course of one year. Culturable bacterial populations were not significantly different between rolABC and wild type lines, whereas the population of fluorescent pseudomonads varied according to the plant lines and the date of s...

文献信息：郭卫东, 周春丽, 路梅, 谢选松.等.  发表于【浙江师范大学学报(自然科学版)】 |  , 请登录

简介：Tender and elder leaf-discs of fingered-citrus (Citrus medicaL. var.sarcodactylis(Noot) Swingle) were transformed withGusgene via biolistics method. The transient expression of Gus genewas used to analyse the transformation rate under different target distance. The results indicated that a higher transformation rate according to transient expression ofGusgene could be acquired from tender leaf-dis...

文献信息：Omar AA, Grosser JW等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

文献信息：Huang JQ, Sun ZH.等.  发表于【Agricultural Sciences in China】 |  , 请登录

简介：The Arabidopsis ICE1(inducer of CBF expression 1)gene was cloned through RT-PCR of Arabidopsis cDNAs and introduced into the lemon(Citrus Limon(L.)Burm.F.cv.Eureka)genome using Agrobacterium-mediated transformation method.Epicotyl segments from in vitro grown lemon seedlings were co-cultivated with A.tumefaciens strain EHA 105 carrying the binary plasmid pMVICE1,whose T-DNA region contain ICE1 gen...