文献信息：Bausher MG, Singh ND, Lee SB, Jansen RK, Daniell H.等.  发表于【BMC Plant Biology】 |  , 请登录

简介：Background: The production of Citrus, the largest fruit crop of international economic value, has recently been imperiled due to the introduction of the bacterial disease Citrus canker. No significant improvements have been made to combat this disease by plant breeding and nuclear transgenic approaches. Chloroplast genetic engineering has a number of advantages over nuclear transformation; it not ...

文献信息：刘歆, 段艳欣, 范净, 邓秀新, 郭文武.等.  发表于【果树学报】 | 请登录

简介：Transformation ofgreen fluorescent protein gene(gfp)intocitrus embryogenic calluses of13 cultivars mediated by Agrobacteriumtumefaciens was conducted.Totally82 transgenic lines from11 cultivars were produced,among which 28 and 25 transgenic lines were obtained from Frost navel orange and Sunburst tangerine respectively.Further PCR analysis of 3 selected transformed cultivars amplified the expected...

文献信息：郑启发, 胡桂兵, 陈大成, 黄自然, 林顺权.等.  发表于【《中国园艺学会第七届青年学术讨论会论文集》】 | 请登录

简介：Base on multiplieation of callus，induetion of somatic embryo and plant regeneration in Ponkan mandarin(Citrus reticulata Blanco cv.‘Ponkan’), the AP1 and AP一BD genes medieated by Agrobacterium tumefaciens were transformed into the callus wounded by carborundum, transformic plantlets were obtained from the callus through seleetion. A. tumefaciens strain LBA4404 harboring pCDB一Ⅱ or p208一AP1 w...

文献信息：F. A. Azevedo, F. A. A. Mourão Filho, B. M. J. Mendes, W. A. B. Almeida, E. H. Schinor, R. Pio, J. M. Barbosa, S Guidetti-Gonzalez, H. Carrer, 等.  发表于【Plant Molecular Biology Reporter】 |  , 请登录

简介：Transgenic plants expressing the bacterio-opsin (bO) gene can spontaneously activate programmed cell death (pcd) and may enhance broad-spectrum pathogen resistance by activating an intrinsic defense pathway in plant species such as tobacco and potato. In this work, we produced transgenic Rangpur lime plants with the bO gene, via Agrobacterium tumefaciens-mediated transformation, and evaluated thes...

文献信息：杨莉, 徐昌杰, 陈昆松.等.  发表于【《中国园艺学会第七届青年学术讨论会论文集》】 |  , 请登录

简介：An effieient in vitro regeneration system and Agrobacterium-mediated transformation system were established in kumquats (Fortunella crassifolia swingle), and more than 20 transgenic plants were obtained. Three protoeols, multiplex PCR,T一DNA flanking sequence analysis and Southern blotting, were applied to early, middle and late analysis of transformation，respectively. With the established transf...

文献信息：韩美丽, 陆荣生, 吴耀军, 杜晓莉.等.  发表于【广西植物】 |  , 请登录

简介：Transformation and regeneration ofCitrusgrandisplants were studied byAgrobacterium-mediated. Transgenic plants with ShivaA gene had been obtained. The carbenicillin was better to controlAgrobacterium growing after explants were infected. Epicotyls fragment were infecting for 10 min as OD600ofAgrobacterium suspending liquid is 0.5. Epicotyls fragment were co-cultured withAgrobacteriumfor 3days. The...

文献信息：周春丽, 郭卫东, 路梅, 陈瑾, 李玉萍等.  发表于【热带亚热带植物学报】 |  , 请登录

简介：Agrobacterium-mediated transformation of Citrus medica L.var.sarcodactlis was performed by leaf disc.The binary vector harbors the gene of interest TPS and NPTII gene driven by CaMV 35S promoter.The presence of TPS gene integration in the genome was detected and confirmed by GUS assay and PCR,respectively. Transformation parameters optimized were bacterial concentration,pre-culture period,immersio...

文献信息：Khawale RN, Singh SK, Garg G, Baranwal VK, Ajirlo SA.等.  发表于【CURRENT SCIENCE】 | 请登录

文献信息：Rai M.等.  发表于【Plant Molecular Biology】 | 请登录

简介：Citrus tristeza virus (CTV) is a major pathogen of Citrus. A single dominant gene Ctv present in the trifoliate relative of Citrus, Poncirus trifoliata confers broad spectrum resistance against CTV. Refinement of genetic maps has delimited this gene to a 121 kb region, comprising of ten candidate Ctv resistance genes. The ten candidate genes were individually cloned in Agrobacterium based binary v...

文献信息：胡春华, 邓子牛, Gentile A, 徐艳, 熊兴耀.等.  发表于【园艺学报】 |  , 请登录

简介：Molecular identification, physiology evaluation and morphological observation of therolA, rolB androlC transgenic citrange clones (B, D, E) were conducted. PCR analysis indicated that therolA, rolB,rolC geneswere integrated into the citrange genome. The expression of the transgenesnptⅡandrolC was detected by usingRT-PCR; while the transgenesrolA androlB did notshow anyRT-PCR produc,t and it wasno...

文献信息：Cevik B, Lee RF, Niblett CL.等.  发表于【Turkish journal of agriculture and forestry】 |  , 请登录

简介：Protein and RNA-mediated forms of pathogen-derived resistance (PDR) have been developed against many viruses in different plants. However, no resistance has been reported against Citrus tristeza virus (CTV), a closterovirus, in Citrus species transformed with coat protein genes or other sequences of CTV. The successful use of replication-associated genes in RNA-mediated resistance in other crops p...

文献信息：Yang L, Xu CJ, Hu GB, Chen KS.等.  发表于【BIOLOGIA PLANTARUM】 | 请登录

文献信息：Yang XH, Sun ZH, Tong RJ.等.  发表于【Agricultural Sciences in China】 |  , 请登录

简介：Genetic transformation experiments of the different explants from Citrus grandis cv. Changshou Shatian You infected with Agrobacterium rhizogenes were carried out in darkness or in light. The optimizing culture system of Ri T-DNA transformed roots for C. grandis cv. Changshou Shatian You was constructed as follows: After the ventral wounded striations on the single activation cotyledon were inocul...

文献信息：Omar AA, Dekkers MGH, Graham JH, Grosser JW等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

文献信息：Trainin, T., Lipsky, A., Levy, A. A., & Holland , D. (2005)等.  发表于【Journal of the American Society for Horticultural Science】 |  , 请登录

简介：The maize transposable element Activator (Ac) has been shown to be active in a number of dicots, including arabidopsis [Arabidopsis thaliana (L.) Heynh.], tobacco (Nicotiana tabacum L.), tomato (Lycopersicon esculentum Mill.), potato (Solanum tuberosum L.), and aspen (Populus tremuloides Michx.). However, no information is available on somatic transposition in any plant during several years of gro...

文献信息：M Cervera，J Juárez，L Navarro，L Peña等.  发表于【Methods in Molecular Biology】 | 请登录

简介：Abstract Most woody fruit species have long juvenile periods that drastically prolong the time required to analyze mature traits. Evaluation of characteristics related to fruits is a requisite to release any new variety into the market. Because of a decline in regenerative and transformation potential, genetic transformation procedures usually employ juvenile material as the source of plant tissue...

文献信息：Takami, K.,Matsumaru, A.,Yahata, M.,Kunitake, H.,Komatsu, H.等.  发表于【Sexual Plant Reproduction】 | 请登录

简介：Embryogenic protoplasts of 'Shogun' mandarin ( Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from Citropsis gabunensis Swing. & M. Kell, and two green embryoids were regenerated after 3 months of culture. Two months after transfer to the regeneration medium, numerous plantlets were obtained from the embryoids. These plants grew vigorously, had well-developed root sys...

文献信息：Viloria, Z.,Grosser, J. W.,Bracho, B.等.  发表于【Plant Cell, Tissue and Organ Culture】 | 请登录

简介：Interploid sexual hybridizations were completed in 2001 and 2002 between seven lemon ( Citrus limon ( L.) Burm. f.) varieties, 'Key' lime ( C. aurantifolia ( Cristm.) Swing), Palestine sweet lime ( C. imettioides Tan.), 'Lakeland' limequat ( C. aurantifolia x Fortunella japonica ( Thumb.) Swing.), and ` Etrog' citron ( C. medica L.) as diploid progenitors and four allotetraploid somatic hybrids ('...

文献信息：Wu, J. H.,Ferguson, A. R.,Mooney, P. A.等.  发表于【Euphytica】 |  , 请登录

简介：Protoplast fusion has been used to produce novel allotetraploid hybrids for use as parents in crosses with diploids to produce easy-peel, seedless, triploid citrus cultivars. Forty three plants were produced from fusions between protoplasts of 'Encore' mandarin and 'Valencia' sweet orange, and of 'Encore' and 'Caffin' clementine mandarin in which protoplasts of each of the donors were isolated fro...

文献信息：Olivares-Fuster, O.,Duran-Vila, N.,Navarro, L.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：We report here the development of a novel protoplast fusion method for citrus somatic hybridization. This new procedure, which we have named electrochemical protoplast fusion, is based on chemically induced protoplast aggregation, using a low concentration of polyethylene glycol, and DC pulse-promoted membrane fusion. Based on the results of nucleus and mitochondria molecular analyses, we were suc...