文献信息：Distefano G, La Malfa S, Vitale A, Lorito M, Deng Z, Gentile A.等.  发表于【Transgenic Research】 |  , 请登录

简介：Constitutive over-expression of antifungal genes from microorganisms involved in plant defence mechanisms represents a promising strategy for conferring genetic resistance against a broad range of plant pathogenic fungi. In the present work, two transgenic lemon clones with the chit42 gene from Trichoderma harzianum were tested for resistance to fungal disease and expression level of defence-relat...

文献信息：段艳欣, 范净, 李鼎立, 郭文武.等.  发表于【华中农业大学学报】 |  , 请登录

简介：To screen the transformants rapidly and efficiently and to obtain putative homozygous transgenie plants in a short time as soon as possible，the Agrobacterium tumefaciens mediated transformarion of embryogenic calli from Ponkan(Citrus reticulata Blanco)，Bingtang sweet orange(C sinensis Osbeck)and Murcott(c．reticulata×C sinensis)were conducted．The sensitivities of embryogenic calli and embryos ...

文献信息：Dutt M, Orbovic V, Barthe G, Pasquali G, Gray D, Grosser J, Li Z.等.  发表于【HORTSCIENCE,】 | 请登录

文献信息：Omar A, Calovic M, El-Shamy H, An HJ, Grosser J.等.  发表于【HORTSCIENCE,】 | 请登录

文献信息：A. Ballester, M. Cervera and L. Pe?a等.  发表于【Plant Cell Reports】 |  , 请登录

简介：The neomycin phosphotransferase (nptII) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants. The present work investigates three different selection alternatives, comparing them to nptII selection in two citrus genotypes, Carrizo citrange...

文献信息：Zou XP, Li DM, Luo XY, Luo KM, Pei Y.等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 |  , 请登录

简介：Highly efficient Agrobacterium-mediated transformation of trifoliate orange (Poncirus trifoliata (L.) Raf.) was achieved via indirect shoot organogenesis. Stable transformants were obtained from epicotyl segments infected with Agrobacterium strain EHA 105 harboring the binary vector pBI121, which contained the neomycin phosphotransferase gene (NPTII) as a selectable marker and the β-glucuronidase...

文献信息：Orbovic V, Calovic M, G. J.等.  发表于【HORTSCIENCE】 | 请登录

文献信息：Rodríguez A, Cervera M, Peris JE, Peña L等.  发表于【Plant Cell, Tissue and Organ Culture】 | 请登录

简介：In citrus, production of mature transgenic plants belonging to different genotypes is an important biotechnological objective. In the present study, we tried to genetically transform and regenerate mature plants from the economically important Navelina sweet orange cultivar by using the procedure previously established for the genetically close Pineapple sweet orange variety. The use of BAP at 3 m...

文献信息：Martinez-Godoy M. A., Mauri N., Juarez J., Marques M. C., Santiago J., Forment J., Gadea J.等.  发表于【BMC Genomics】 | 请登录

简介：Background: Understanding of genetic elements that contribute to key aspects of citrus biology will impact future improvements in this economically important crop. Global gene expression analysis demands microarray platforms with a high genome coverage. In the last years, genomewide EST collections have been generated in citrus, opening the possibility to create new tools for functional genomics i...

文献信息：Levy, A., Erlanger, M., Rosenthal, M. and Epel, B. L.等.  请登录

简介：Plasmodesmal conductivity is regulated in part by callose turnover, which is hypothesized to be determined by beta-1,3-glucan synthase versus glucanase activities. A proteomic analysis of an Arabidopsis thaliana plasmodesmata (Pd)-rich fraction identified a beta-1,3-glucanase as present in this fraction. The protein encoded by the putative plasmodesmal associated protein (ppap) gene, termed AtBG_p...

文献信息：Orbovic, V., Pasquali, G., Grosser, J. W.等.  发表于【Acta Horticulturae】 |  , 请登录

文献信息：Mourao, F. D. A.,Pio, R.,Mendes, B. M. J.,de Azevedo, F. A.,Schinor, E. H., Entelmann, F. A., Alves, A. S. R. , Cantuarias-Aviles, T. E. 等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Somatic hybridization is a biotechnology tool that can be used in citrus breeding programs to produce somatic hybrids with the complete genetic combination of both parents. The goal of this work was to test the reaction of citrus somatic hybrids that may be useful as rootstocks to trunk and root infections caused by Phytophthora nicotianae van Breda de Haan (P parasitica Dastur) and to citrus tris...

文献信息：Tan, M. L.,Song, J. K.,Deng, X. X.等.   , 请登录

简介：Intergeneric sexual hybridizations were conducted between two genera of Rutaceae, with Satsuma mandarin (Citrus unshiu Marc) and Red tangerine (C. reticulata Blanco) as maternal parents, and Poncirus trifoliata (L.) Raf as the paternal parent, in an effort to generate hybrid populations for both molecular mapping and rootstock breeding. Embryo rescue is important for citrus sexual breeding because...

文献信息：Pavan, Alexandra,Calixto, Marcia Cristina,Cardoso, Suane Coutinho, Januzzi Mendes, Beatriz Madalena, Bergamin Filho, Armando, Spotti Lopes, Joao等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Asiatic citrus canker (ACC), caused by Xanthomonas axonopodis Starr & Garces pv. citri (Hasse) Vauterin et al., and citrus variegated chlorosis (CVC), caused by Xylella fastidiosa Wells et al., are considered the main diseases affecting sweet orange scion varieties in Brazil. Among commercial varieties, mandarins and tangerines are recognized as tolerant to these pathogens. We report herein the pr...

文献信息：Olivares-Fuster, O.,Hernandez-Garrido, M.,Guerri, J.,Navarro, L.等.  发表于【Tree Physiology】 | 请登录

简介：Unlike maternal inheritance in sexual hybridization, plant somatic hybridization allows transfer, mixing and recombination of cytoplasmic genomes. In addition to the use of somatic hybridization in plant breeding programs, application of this unique tool should lead to a better understanding of the roles played by the chloroplastic and mitochondrial genomes in determining agronomically important t...

文献信息：Guo, W. W.,Wu, R. C.,Cheng, Y. J.,Deng, X. X.等.   , 请登录

简介：Somatic hybridization has been an effective and successful technique for plant improvement. In this paper, embryogenic callus protoplasts of red tangerine (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from citrange (C. sinensis x P. trifoliata, a Chinese local strain) in an effort to produce complementary tetraploid citrus rootstocks. Regenerated embryoids grew slow...

文献信息：Grosser, J. W.,Chandler, J. L.,Duncan, L. W.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Sting nematode (Belonolaimus longicaudatus Rau) has become a primary factor limiting citrus production in localized regions of the central Florida sandridge citrus production area, making the development of resistant rootstocks a new breeding objective. In efforts to develop a replacement rootstock for the widely adapted sour orange, our focus has been on somatic hybridization of selected mandarin...

文献信息：Cai, X. D.,Fu, J.,Deng, X. X.,Guo, W. W.等.  发表于【Plant Cell, Tissue and Organ Culture】 |  , 请登录

简介：Cytoplasmic male sterility (CMS) is known to be controlled by mitochondrial genome in higher plants including Satsuma mandarin (Citrus unshiu Marc.). Citrus symmetric fusion experiments often produce diploid cybrids possessing nuclear DNA from the mesophyll parent and mitochondrial DNA (mtDNA) from the embryogenic callus parent. Therefore, it is possible to transfer CMS from Satsuma mandarin as ca...

文献信息：刘继红等.  发表于【湖北省植物生理学会第十五次学术研讨会】 |  , 请登录

简介：柑桔是一种重要的果树,由于具有多胚性、遗传高度杂合和童期长等特点,传统育种技术在柑桔品种改良上的进程受到阻碍,采用生物技术手段进行遗传改良具有重要的意义。原生质体融合是克服上述困难创造新种质的重要手段,自1985年获得首例柑桔体细胞杂种以来,已得到300余例多种倍性的柑桔体细胞杂种,柑桔体细胞杂种的鉴定主要采用形态学、细胞学和分子标记完成,并且对部分体细胞杂种的核质组成进行了详细分析,体细胞杂种的应用主要表现在接...

文献信息：郭文武,陈春丽,邓于洋,邓秀新等.  发表于【园艺学报】 |  , 请登录

简介：二倍体锦橙+种间四倍体体细胞杂种HR(哈姆林甜橙+粗柠檬)的种间六倍体体细胞杂种植株移入温室7年后(即2004年),有1株六倍体首次开花,花粉染色活力40.8%,花粉萌发率3.7%;2006年该植株再次开花,花粉染色活力为20.3%。由于自然界中没有柑橘六倍体,本研究是人工的方法创造出可育的柑橘六倍体体细胞杂种植株的首例报道。