文献信息：Yang L, Xu CJ, Hu GB, Chen KS.等.  发表于【BIOLOGIA PLANTARUM】 | 请登录

文献信息：Fagoaga C, Tadeo FR, Iglesias DJ, Huerta L, Lliso I, Vidal AM, Talon M, Navarro L, García-Martínez JL, Peña L.等.  发表于【Journal of Experimental Botany】 |  , 请登录

简介：Carrizo citrange (Citrus sinensis3Poncirus trifoliata) is a citrus hybrid widely used as a rootstock, whose genetic manipulation to improve different growth characteristics is of high agronomic interest. In this work, transgenic Carrizo citrange plants have been produced overexpressing sense and antisense CcGA20ox1 (a key enzyme of GA biosynthesis) under control of the 35S promoter to modify plant...

文献信息：Duan YX, Guo WW, Meng HJ, Tao NG, Li DD, Deng XX.等.  发表于【Biologia Plantarum】 |  , 请登录

简介：Transformation and high efficient regeneration of transgenic plants from embryogenic calluses of Bingtang sweet orange [Citrus sinensis (L.) Osbeck] was reported. Embryogenic calluses were inoculated with Agrobacterium tumefaciens strain EHA105, harboring the binary Ti plasmid pROK II and carrying a neomycin phosphotransferase II (NPTII) gene, an intron β-glucuronidase (GUS) gene and the Arabidop...

文献信息：Tan FC, Swain SM.等.  发表于【Physiologia Plantarum】 |  , 请登录

简介：Flowering and flower formation are defining features of angiosperms and the control of these developmental processes involves a common repertoire of genes which are shared among different species of flowering plants. These genes were first identified using various homeotic and flowering time mutants of Arabidopsis and snapdragon, and homologous genes have subsequently been isolated from a wide ran...

文献信息：Omar AA, Grosser JW.等.  发表于【Acta Horticulturae.】 | 请登录

文献信息：Chen CX，Zheng QF，Xiang X，Soneji JR，Huang S，Choi YA，Rao MN，Gmitter FG.等.  发表于【HORTSCIENCE】 | 请登录

文献信息：胡新喜, 敖小平, 邓子牛, 熊兴耀.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：Using epicotyl and hypocotyl pieces and cotylcdons of Citrus sinensis Osbeck cv．Dahong seedlings grown in vitro as explants，an efficient regeneration system was establlished. The optimal conditions were obtained as follows. 1) The epicotyl pieces were used as explants．2)The optimal advendtitious shoot regeneration medium was MS salts+100mg/L inositol +0.2 mg/L V-B1+1 mg/L V-B6+1 mg/L niacin ...

文献信息：Guo, W. W.,Cheng, Y. J.,Chen, C. L.,Deng, X. X.等.  发表于【Scientia Horticulturae】 | 请登录

简介：More than 150 plants were regenerated from our previous somatic hybridization between embryogenic callus line of Page tangelo (Citrus reticulata Blanco x C. paradisi Macf) and mesophyll protoplasts of rough lemon (C. jambhiri Lush) mediated by electrofusion. Preliminary screening showed that 78% of these plants were tetraploids while the rest were diploids morphologically resembling the leaf paren...

文献信息：Xu, X. Y,Liu, J. H.,Deng, X. X.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：Cytoplasm of Satsuma mandarin (Citrus unshiu Marc.) is known to influence seedlessness. Transfer of cytoplasm to a seedy cultivar could possibly lead to the production of seedless citrus fruits. In the present paper cytoplasts were isolated from cell suspension-derived protoplasts of Satsuma mandarin via ultra-centrifugation in a discontinuous gradient. No nucleus could be detected in the cytoplas...

文献信息：Cai, X. D.,Liu, X.,Guo, W. W.等.  发表于【Plant Cell, Tissue and Organ Culture】 | 请登录

简介：Somatic hybridization was performed via electrofusion between embryogenic suspension-derived protoplasts of transgenic green fluorescent protein (GFP) Satsuma mandarin (Citrus unshiu Marc. cv. Guoqing No. 1) (G1) callus and mesophyll protoplasts of calamondin (Citrus microcarpa Bunge), and three embryoids expressing GFP under UV light were obtained after 60 days of culture. The three embryoids wer...

文献信息：Ananthakrishnan, G.,Calovic, M.,Serrano, P.,Grosser, J. W.等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 |  , 请登录

简介：Sour orange (Citrus aurantium L.) rootstock has historically been a widely utilized citrus rootstock throughout the world due to its wide soil adaptability and superior horticultural performance. However, quick-decline isolates of citrus tristeza virus (CTV) have demolished entire industries of sour orange rootstock in some countries, including Brazil and Venezuela. CTV is presently destroying mil...

文献信息：Carimi, F.,de Pasquale, F.,Puglia, A. M.等.  发表于【Plant Breeding】 | 请登录

简介：Embryo development in vivo has been studied in four Citrus aurantium L. polyembryonic genotypes. Seeds were collected 65, 85, 105, 125 and 220 days after pollination (DAP). None of the immature seeds harvested 65 and 85 DAP contained visible embryos. A single embryo at a more advanced developmental stage was observed in the central position at the micropylar apex of the embryo sac in about 74% of ...

文献信息：刘继红等.  2006-11-21 发表于【第二届热带亚热带植物资源的遗传多样性与基因发掘利用研讨会】 |  , 请登录

简介：柑桔是一种重要的果树,由于具有多胚性、遗传高度杂合和童期长等特点,传统育种技术在柑桔品种改良上的进程受到阻碍,采用生物技术手段进行遗传改良具有重要的意义。原生质体融合是克服上述困难创造新种质的重要手段,自1985年获得首例柑桔体细胞杂种以来,已得到300余例多种倍性的柑桔体细胞杂种,柑桔体细胞杂种的鉴定主要采用形态学、细胞学和分子标记完成,并且对部分体细胞杂种的核质组成进行了详细分析,体细胞杂种的应用主要表现在接...

文献信息：洪柳等.  请登录

简介：体细胞突变是柑橘等果树比较常见的生物学现象，也是品种选育，即芽变选种的主要变异来源。针对柑橘芽变品种较多，识别比较困难，遗传标记差异小等困难，本文采用MSAP(甲基化敏感扩增多态性)等标记技术，对世界主要的甜橙类型脐橙(Citrus sinensis)不同品种进行分析，研究其遗传多样性；同时，利用体细胞的倍性变异以及柑橘具有珠心胚现象的特点，针对椪柑种子多的问题，从椪柑种子离体播种实生苗中分离得到四倍体，为培育三倍体无核...

文献信息：向旭等.  发表于【分子植物育种】 |  , 请登录

简介：本文综述了柑桔抗病分子育种近年来的研究动态和所取得的研究成果。柑桔分子育种始于上世纪90年代,以期克服常规育种面临的多方面严重阻碍,如杂种不育、杂交不亲和、较长的童期和大的树体积,以及随之而来的用地和巨大的人力、物力、成本等;其相对较小的基因组已使其成为果树分子育种研究领域的模式植物之一。首先在利用富有目标抗病基因的柑桔近缘属枳(Poncirustrifoliate)作亲本,培育杂种群体,开发分子标记与连锁遗传作图等方面取得...

文献信息：陈方永,罗君琴等.  2006-07-05 发表于【浙江农业科学】 |  , 请登录

简介：以异源四倍体体细胞杂种为父本,与单胚性二倍体柑橘品种玉环柚进行有性杂交,获得玉环柚三倍体植株。试验发现,玉环柚授粉后80 d为最佳幼胚离体培养时间。幼胚在无菌条件下进行胚抢救离体培养,直接萌发成苗或发展成为畸形胚,后者进一步诱导可形成增殖丛芽。试管芽经生根、炼苗,获得了58棵完整植株。用流式细胞仪对其倍性进行检测,结果表明,8株为三倍体(2n=3x=27),49株为二倍体(2n=2x=18),1株为三倍体嵌合体。可见,异源四倍体与二倍体种...

文献信息：伊华林,荣融等.  发表于【果树学报】 |  , 请登录

简介：对柑橘体细胞杂种有性后代叶形态遗传变异研究表明,有性后代群体叶长、叶宽、叶面积平均值低于双亲,叶厚与双亲接近,而气孔长、宽与密度平均值介于双亲之间;不同有性后代植株叶长、叶宽、叶面积、叶厚及气孔长、宽与密度变化范围与杂交双亲对应的参数呈交叉现象,二倍体与三倍体群体间没有明显分组。有性后代除叶厚变异范围较小外,其它参数变异范围都较大。经次数资料的适合性测验即χ2测验,叶形、叶尖缺刻的有无及翼叶形状分离符合1∶...

文献信息：张敏,邓秀新等.  发表于【果树学报】 |  , 请登录

简介：综述了柑橘芽变选种的成果。近年来,我国选育了崀丰、长红、新世纪、奉晚等脐橙品种和华柑2号椪柑、岩溪晚芦、光明早温州蜜柑等宽皮柑橘品种,还有锦橙101、红肉琯溪蜜柚等其他柑橘品种。介绍了RAPD、AFLP、ISSR、MSAP和基于反转录转座子的分子标记在柑橘芽变品种鉴别中的应用。阐述了部分柑橘芽变性状形成的细胞学机理和分子机理,指出目前对芽变的研究主要集中在反转录转座子的插入、DNA甲基化、基因结构和表达差异等方面。结合果树...

文献信息：刘永忠等.   , 请登录

简介：奉节晚橙(Citrus sinensis Osbeck)是一个来源奉节72-1脐橙的晚熟芽变品种。这两个品种一起将构成一个很好的柑橘成熟机理研究体系。加强对奉节晚橙的研究，明确奉节晚橙晚熟芽变发生的机理，将为柑橘熟期育种奠定理论基础，最终有助于柑橘产业的持续健康发展。本文研究了奉节晚橙与奉节72-1脐橙在果实成熟过程中的糖酸、色素、以及转录水平的变化，探讨了晚熟芽变脐橙发生原因，取得如下主要结果： 1．建立了一种适合于柑橘不同时期...

文献信息：郑启发, 胡桂兵, 陈大成, 黄自然, 林顺权.等.  发表于【 中国园艺学会第七届青年学术讨论会】 | 请登录

简介：对农杆菌介导法转化沙田柚(Citrus grandis Osbeck cv．Shatian pummelo)下胚轴得到的转抗菌肽D(AP-D)基因的单株(T0H7)进行了无性繁殖并对无性繁殖系进行了AP-D基因的整合和表达检测以及柑橘溃疡病病原菌的攻毒试验。结果表明:AP-D基因能在无性系中稳定整合与表达；PCR和Southern blot结果证实在植株中以单拷贝存在；大田溃疡病自然发病观测和针刺接种法攻毒试验结果显示，转基因沙田柚对溃疡病表现了一定的抗病性，但不同芽株(或株...