文献信息：罗赛男, 邓子牛, 钟晓红, 张家银, 袁飞荣, 杨莉.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：A parthenocarpy gene(DefH9-iaaM)was introduced into Succari to obtain possible seedless transformants, by using internodal stem segments as explants to establish an efficient regeneration and transgenic system.The results indicated that 30-day-old seedlings were the most efficient explants.The co-culture in darkness for 3~6 days gave a rise to good regeneration.The optimum medium for adventitious ...

文献信息：罗赛男, 邓子牛, 钟晓红, 易图永, 黄荣峰, 曾柏全.等.  发表于【中南林业科技大学学报】 |  , 请登录

简介：An experiment was conducted by inoculating citrus bacterial canker and citrus anthracnose to the leaves of transgenie Succari．Results show that TERFl gene can enhance the transgenic plants with their broad—spectrum disease resistance；that after an inoculation with citrus bacterial canker, their incidence rate is reduced by 50％，that compared tO Bingtang Orange and CK, transgenic Succari also d...

文献信息：罗赛男, 钟晓红, 邓子牛, 黄容峰.等.  发表于【江西农业大学学报】 |  , 请登录

简介：After establishing the regeneration and transformation systems of citrus, the TERF1 gene was mediated into ssweetorange byEHA105 and LB4404Agrobacterium tumefaciens. Seven plantswere verified by PCR and RT-PCR amplification. The results showed that EHA105 had more efficiency in improving the transgenic ratio than LBA440

文献信息：Batuman O, Mawassi M, Dawson WO, Bar Joseph M.等.  发表于【INDIAN JOURNAL OF VIROLOGY】 | 请登录

文献信息：Kunta M, Skaria M, Da Graca JV, Mirkov T, Louzada ES.等.  发表于【PHYTOPATHOLOGY】 | 请登录

文献信息：谢玉明, 郭琛, 张家银, 杨丽, 胡春华, 邓子牛.等.  发表于【分子植物育种】 |  , 请登录

简介：Genetic transformation systems from mature tissue of citrus have to be achieved as a necessary requiremerit for the introduction of useful genes into specific cultivars and the rapid evaluation of the genetically modifled characteristic．This research presents a protoeol for in vitro regeneration and genetic transformation of C. sinensis Osbesk cv Newhall using as explants intemodal segments from ...

文献信息：Sala Junior V, Celloto VR, Vieira LGE, Gonçalves JEG, Gonçalves RAC, Oliveira AJB.等.  发表于【Plant Systematics and Evolution】 | 请登录

简介：Floral nectar chemical composition of conventional and transgenic sweet orange trees expressing the antibacterial peptide sarcotoxin IA (Stx IA), which has been shown to efficiently inhibit in vitro growth of the bacteria responsible for citrus canker (Xanthomonas axonopodis pv. citri), is reported. The nectar contents of primary metabolites (sugars, amino acids, proteins, triacylglycerides) and o...

文献信息：谢玉明, 郭琛, 张家银, 韩健, 袁飞荣, 邓子牛.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：The phyB gene was transferred into the genome of Poncirus trifoliate in vitro with explants of epicotyl mediated by Agrobacterium．To诅l 27 strains with plant resistance plants were regenerated．The presence of specific targeted strips of phyB gene in the 5 regenerated plants Was confirmed by PCR analysis．RT-PCR analysis indicated the regular expression of transgene phyB in two transgenic clones，...

文献信息：Omar AA, Dekkers MG, Graham JH, Grosser JW.等.  发表于【Biotechnology Progress】 |  , 请登录

简介：Quantitative real-time PCR(qRT-PCR) was adapted to estimate transgene copy number of the rice Xa21 gene in transgenic citrus plants. This system used TaqMan qRT-PCR and the endogenous citrus gene encoding for lipid tansfer protein(LTP). Transgenic 'Hamlin' sweet orange plants were generated using two different protoplast-GFP transformation systems: cotransformation and single plasmid transformatio...

文献信息：Terol J, Naranjo MA, Ollitrault P, Talon M.等.  发表于【BMC Genomics】 |  , 请登录

简介：Background: Citrus species constitute one of the major tree fruit crops of the subtropical regions with great economic importance. However, their peculiar reproductive characteristics, low genetic diversity and the long-term nature of tree breeding mostly impair citrus variety improvement. In woody plants, genomic science holds promise of improvements and in the Citrus genera the development of ge...

文献信息：Febres VJ, Lee RF, Moore GA等.  发表于【Plant Cell Reports】 | 请登录

简介：Grapefruit (Citrus paradisi) transgenic plants transformed with a variety of constructs derived from the Citrus tristeza virus (CTV) genome were tested for their resistance to the virus. Most transgenic lines were susceptible (27 lines), a few were partially resistant (6 lines) and only one line, transformed with the 30 end of CTV was resistant. Transgene expression levels and siRNA accumulation w...

文献信息：Cervera M, Navarro A, Navarro L, Peña L.等.  发表于【Tree Physiology】 | 请登录

简介：Genetic transformation of mature trees is difficult because adult tissues are recalcitrant to Agrobacterium tumefaciens infection and transformation and because transgenic mature events are less competent for regeneration. We have shown that reinvigoration allows manipulation of the vegetative phase to increase the potential for transformation and regeneration without loss of competence for flower...

文献信息：Mendes AFD, Cidade LC, Manzoli GN, Otoni WC, Soares WD, Costa MGC.等.  发表于【Pesquisa Agropecuária Brasileira】 |  , 请登录

简介：The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotyp...

文献信息：Guo WW, Duan YX, Li DL, Liu X, Tan B, Cai XD, Grosser JW, Deng XX.等.  发表于【Acta Horticulturae】 | 请登录

文献信息：Luro FL, Costantino G, Terol J, Argout X, Allario T, Wincker P, Talon M, Ollitrault P, Morillon R.等.  发表于【BMC Genomics】 |  , 请登录

简介：Background: During the last decade, numerous microsatellite markers were developed for genotyping and to identify closely related plant genotypes. In citrus, previously developed microsatellite markers were arisen from genomic libraries and more often located in non coding DNA sequences. To optimize the use of these EST-SSRs as genetic markers in genome mapping programs and citrus systematic analy...

文献信息：张觅, 罗小英, 白文钦, 李怡然, 侯磊, 裴炎, 李忠旺, 李德谋.等.  发表于【园艺学报】 |  , 请登录

简介：Malate, ubiquitously existing in plan,t plays a crucial role in variousmetabolic pathways, including respiration, nitrogen fixation, phosphorus acquisition, and aluminum tolerance. Malate dehydrogenase(MDH) catalyzes the interconversion ofoxaloacetate andmalate. Previous results suggested that overexpressing MDHgene transgenic plants elevated tolerance to aluminum toxicity.Citrus junos, one ofmain...

文献信息：Omar A, Dutt M, Barthe G, Orbovic V, Grosser J.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

文献信息：Omar AA, Grosser JW.等.  发表于【Plant Science】 |  , 请登录

简介：In order to improve transformation efficiency in citrus two green fluorescent protein (GFP) constructions, an endoplasmic reticulum targeted (GFP-ER) and cytoplasmic targeted (Cy-GFP), were used to study expression and stability of GFP in transgenic plants. Results from citrus protoplast-GFP transformation showed that GFP-ER had brighter fluorescence than the Cy-GFP. Although both of these constru...

文献信息：Andreote FD, Rossetto PB, Souza LCA, Marcon J, Maccheroni W, Azevedo JL, Araujo WL.等.  发表于【Journal of Basic Microbiology】 | 请登录

简介：Harmless bacteria inhabiting inner plant tissues are termed endophytes. Population fluctuations in the endophytic bacterium Pantoea agglomerans associated with two species of field cultured citrus plants were monitored over a two-year period. The results demonstrated that populations of P. agglomerans fluctuated in Citrus reticulata but not C. sinensis. A cryptic plasmid pPA3.0 (2.9 kb) was identi...

文献信息：M. L. P. Oliveira, V. J. Febres, M. G. C. Costa, G. A. Moore and W. C. Otoni等.  发表于【Plant Cell Reports】 | 请登录

简介：An improved method for the Agrobacterium infiltration of epicotyl segments of ‘Pineapple’ sweet orange [Citrus sinensis (L.) Osbeck] and ‘Swingle’ citrumelo [Citrus paradisi Macf. X Poncirus trifoliata (L.) Raf.] was developed in order to increase transformation frequency. Sonication-assisted Agrobacterium-mediated transformation (SAAT), vacuum infiltration, and a combination of the two proced...