文献信息：Nishikawa F, Endo T, Shimada T, Fujii H, Shimizu T, Kobayashi Y, Araki T, Omura M.等.  发表于【Tree Physiology】 |  , 请登录

简介：Ectopic expression of a Flowering Locus T (CiFT) from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.). To profile transcriptional effects of CiFT, mRNA extracted from the shoots of the transgenic trifoliate orange was subjected to genetic analysis using a 22-K oligo DNA microarray. Microarray results and reverse transcription polymerase chain reaction...

文献信息：覃 磊, 袁飞荣, 曹雄军, 罗赛男, 邓子牛.等.  发表于【中国园艺文摘】 |  , 请登录

简介：The effect of drought stress on the transgenic sweet orange with TERF1 and wild type were researched. The results showed that the leaves of wild type were wilting with the increase of drought stress and the ability of photosynthesis was declining. Until severe drought stress (SRWC=10%), leaves shed and growth tends stop with the chlorophyll content decreased to 50.0%. While the leaves of transgeni...

文献信息：Singh S, Rajam MV.等.  发表于【JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY】 | 请登录

文献信息：Zhang X, Francis MI, Dawson WO, Graham JH, Orbović V, Triplett EW, Mou Z.等.  发表于【European Journal of Plant Pathology】 |  , 请登录

简介：Citrus canker, caused by the bacterial pathogen Xanthomonas citri subsp. citri (Xcc), is a serious leaf and fruit spotting disease affecting many important citrus cultivars including grapefruit and certain sweet oranges. Currently, efficacious and economical disease control measures for highly susceptible citrus cultivars are lacking. Development of commercial cultivars with greater field resistan...

文献信息：Dutt M, Grosser JW.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：A method for the genetic transformation of several citrus cultivars is described, including cultivars observed to be recalcitrant to conventional epicotyl-mediated transformation. Embryogenic cell suspension cultures, established from unfertilized ovules were used as target tissues for Agrobacterium-mediated transformation. Several modifications were made to the culture environment to investigate ...

文献信息：Cervera M, Esteban O, Gil M, Gorris MT, Martínez MC, Peña L, Cambra M.等.  发表于【Transgenic Research】 |  , 请登录

简介：Citrus tristeza virus (CTV) causes one of the most destructive viral diseases of citrus worldwide. Generation of resistant citrus genotypes through genetic engineering could be a good alternative to control CTV. To study whether production of single-chain variable fragment (scFv) antibodies in citrus could interfere and immunomodulate CTV infection, transgenic Mexican lime plants expressing two di...

文献信息：Cardoso SC, Barbosa-Mendes JM, Boscariol-Camargo RL, Christiano RSC, Filho AB, Vieira MLC, Mendes BMJ, Mourão Filho FAA.等.  发表于【Plant Molecular Biology Reporter】 |  , 请登录

简介：Genetic transformation with genes that code for antimicrobial peptides has been an important strategy used to control bacterial diseases in fruit crops, including apples, pears, and citrus. Asian citrus canker (ACC) caused by Xanthomonas citri subsp. citri Schaad et al. (Xcc) is a very destructive disease, which affects the citrus industry in most citrus-producing areas of the world. Here, we repo...

文献信息：胡新喜, 敖小平, 邓子牛, 熊兴耀.等.  发表于【中国农学通报】 |  , 请登录

简介：Transgenic plants appear to be a new way to citrus improvement and a powerful tool for studying gene functions. The establishment of an efficient in vitro plant regeneration system suitable for genetic transformation is the first step in this approach. Different explants of seedlings were grownin vitro and a efficient regeneration system was established for Citrus grandis Osbeck cv. Shatianyou. Th...

文献信息：严佳文, 袁飞荣, 龙桂友, 覃磊, 邓子牛.等.  发表于【园艺学报】 |  , 请登录

文献信息：López C, Cervera M, Fagoaga C, Moreno P, Navarro L, Flores R, Peña L.等.  发表于【Molecular Plant Pathology】 |  , 请登录

简介：Mexican lime plants transformed with the 3′-terminal 549 nucleotides of the Citrus tristeza virus (CTV) genome in sense, antisense and intron-hairpin formats were analysed for transgenederived transcript and short interfering RNA (siRNA) accumulation, and for CTV resistance. Propagations from all sense, antisense and empty-vector transgenic lines were susceptible to CTV, except for a single sense...

文献信息：Mendes BMJ, Cardoso SC, Boscariol-Camargo RL, Cruz RB, Mourão Filho FAA, Bergamin Filho A.等.  发表于【Plant Pathology】 |  , 请登录

简介：This work reports the evaluation of citrus canker resistance in transgenic lines of four sweet orange cultivars Hamlin, Natal, Pera and Valencia expressing the rice Xa21 R-gene. The Agrobacterium-mediated genetic transformation was performed and transgene integration was confirmed by Southern blot analyses. These analyses showed that the plants had undergone 1–3 independent transgene integration ...

文献信息：Mondal SN, Dutt M, Barthe GA, Grosser JW, Dewdney MM.等.  发表于【PHYTOPATHOLOGY】 | 请登录

文献信息：李娜, 严佳文, 覃磊, 曾伯全, 邓子牛.等.  发表于【园艺学报】 |  , 请登录

文献信息：Ballester A, Cervera M, Peña L.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：Selectable marker (SM) genes have been considered necessary to achieve acceptable rates in the generation of transgenic plants. Genes encoding antibiotic or herbicide resistance are widely used for this purpose. In most cases, once transgenic plants have been regenerated, permanence of SM genes in the plant genome is no longer necessary, and it becomes a matter of public concern. Moreover, the rem...

文献信息：Wang CX, Hong N, Wang GP, Jiang B, Fan XD等.  发表于【Journal of Plant Pathology 】 |  , 请登录

简介：Citrus tristeza virus isolate YC (CTV-YC), a stem pitting-inducing strain, was graft-transmitted to seedlings of Mexican lime [Citrus aurantifolia (Christm.)Swingle], Pineapple sweet orange [C. sinensis (L.) Osbeck] and Arizona Etrog citron 861-SI (C. medica L.).Nodal stem segments from CTV-YC-infected and healthy seedlings of the three species were used as explants for regeneration in vitro. CTV ...

文献信息：Bassene JB, Froelicher Y, Dubois C, Ferrer RM, Navarro L, Ollitrault P, Ancillo G.等.  发表于【Heredity】 | 请登录

简介：Polyploid plants often produce new phenotypes, exceeding the range of variability existing in the diploid gene pool. Several hundred citrus allotetraploid hybrids have been created by somatic hybridization. These genotypes are interesting models to study the immediate effects of allopolyploidization on the regulation of gene expression. Here, we report genome-wide gene expression analysis in fruit...

文献信息：van Vuuren, S. P.,Manicom, B. Q.等.  发表于【South African Journal of Plant and Soil】 | 请登录

简介：Huanglongbing remains a threat in the cooler citrus production areas of South Africa despite restrictions on the movement of citrus material from infected areas, cultural control measures, planting of healthy certified trees and the eradication of infected material. The ultimate control strategy would be the use of resistant plant material. Attempts to obtain resistance with conventional breeding ...

文献信息：Cai, X. D.,Fu, J.,Chen, C. L.,Guo, W. W.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Somatic hybridization provides an alternative for transferring mitochondria-encoded cytoplasmic male sterility (CMS). Herein, symmetric protoplast electrofusion was conducted between embryogenic callus protoplasts of Citrus unshiu Marc. cv. Guoqing No. 1 (G1), a CMS cultivar, and mesophyll-derived protoplasts of seedy 'Page' tangelo [C. reticulata Blanco x (C. reticulata Blanco x C. paradisi Macf....

文献信息：郭文武,蔡小东,付婧,彭志军,徐世晓,邓秀新等.  发表于【2009中国植物学会植物细胞生物学学术年会】 |  , 请登录

简介：果实无核是鲜食柑橘品质优良的重要标志,我国有近一半的柑橘为有核类型,采用细胞工程技术进行我国特色有核柑橘品种的无核化遗传改良具有重要意义。在获得大量异源四倍体体细胞杂种和二倍体胞质杂种并对其进行核质遗传分析的基础上,近年提出了柑橘二倍体无核

文献信息：杨亚婷等.   , 请登录

简介：辐射诱变育种是创造果树新种质的一个有效途径。它不但可以大大提高基因突变频率,而且可以产生小量突变,在改良品种的同时又不会改变原有品种的固有优质性状,从而可能获得常规育种难以获得的新种质。辐射诱变育种与离体培养结合能大大降低嵌合体的比例,可在有限的空间内对大量诱变群体进行选择,提高了后代的筛选、鉴定效率。本实验利用~(60)Coγ射线辐射柑橘品种伏令夏橙(Citrus sinensis Osbeck)和江西无核椪柑(C.reticulata Blanco...