文献信息：Niedz RP, Evens TJ.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

文献信息：Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW.等.  发表于【Tree Genetics & Genomes】 | 请登录

简介：Precocious trifoliate orange (Poncirus trifoliata [L.] Raf), an extremely early flowering mutant of P. trifoliata, is an attractive model for functional genomics research in Citrus. A procedure for efficient regeneration and transformation of this genotype was developed by using green fluorescent protein (GFP) gene as visual marker and etiolated stem segments as explants. In vivo monitoring of GFP...

文献信息：Dutt M, Barthe G, Francis M, Graham J, Grosser J.等.  发表于【PHYTOPATHOLOGY】 | 请登录

文献信息：谭彬, 郭文武.等.  发表于【《第二届全国果树分子生物学学术研讨会论文集》】 | 请登录

文献信息：Matsuda N, Ikeda K, Kurosaka M, Takashina T, Isuzugawa K, Endo T, Omura M.等.  发表于【Journal of the Japanese Society for Horticultural Science】 |  , 请登录

简介：We transformed the European pear (Pyrus communis L.) ‘La France’ and ‘Ballade’ with the Citrus FLOWERING LOCUS T (CiFT) gene, which induces early flowering. Subsequent DNA blot analysis of the transformed and wild-type plants indicated that the transformed plants carried 1–4 copies of CiFT. Of the 8 transformed ‘La France’ and 7 transgenic ‘Ballade’ plant lines obtained, 7 and 5 lines res...

文献信息：Koca U, Berhow MA, Febres VJ, Champ KI, Carrillo-Mendoza O, Moore GA.等.  发表于【Physiologia Plantarum】 | 请登录

简介：Citrus species accumulate large quantities of flavanone glycosides in their leaves and fruit. The physiological role(s) of these compounds in citrus plants are unknown, but they have been documented to benefit human health upon consumption. Flavanone rutinosides are tasteless, whereas flavanone neohesperidosides, such as naringin, give a bitter taste to fruit and fruit juice products, reducing the...

文献信息：Cervera M, Navarro L, Peña L等.  发表于【Journal of Biotechnology】 |  , 请登录

简介：Rapid flowering is crucial to perform functional genomic studies to investigate reproductive biology characteristics and fruit quality-related traits in fruit trees. However, long generation cycles of woody plants considerably delay this evaluation. Through genetic transformation, juvenile periods can be significantly shortened by overexpression of flower meristem-identity genes. Transgenic APETAL...

文献信息：Bowman KD, McCollum G, Niedz R, Stover E, Albrecht U, Stange R.等.  发表于【HORTSCIENCE】 | 请登录

文献信息：Vibhute M, Tiwari R, Tripathi MK, Baghel BS, Tiwari S.等.  发表于【Plant Cell Biotechnology and Molecular Biology】 | 请登录

文献信息：Dutt, Manjul Orbovi, Vladimir Grosser, Jude W.等.  发表于【Proceedings of the ... annual meeting of the Florida State H】 | 请登录

文献信息：Endo T, Shimada T, Fujii H, Nishikawa F, Sugiyama A, Nakano M, Shimizu T, Kobayashi Y, Araki T, Peña L, Omura M.等.  发表于【Journal of the Japanese Society for Horticultural Science】 |  , 请登录

简介：We have developed a CiFT co-expression system for the rapid evaluation of transgenic citrus flowers and fruit. In order to co-express a target gene and the CiFT gene, a CiFT co-expression vector was constructed by inserting the fragment containing a chimeric target gene into the binary vector harboring P35S::CiFT. This system was applied to metabolically engineer aroma components in trifoliate ora...

文献信息：付行政, 陈传武, 王引, 刘继红等.  发表于【《第二届全国果树分子生物学学术研讨会论文集》】 |  , 请登录

文献信息：LI D. L., TAN B., DUAN Y. X., GUO W. W.等.  发表于【Biologia Plantarum】 |  , 请登录

简介：Transformation and high efficient regeneration of transgenic plants from the trimmed etiolated shoot/root region (TESRR) of Anliucheng sweet orange [Citrus sinensis (L.) Osb.] seedling was reported. A visual green fluorescent protein (GFP) marker gene was introduced to evaluate transformation efficiency by using the explants from TESRR and epicotyls. The transformation protocol was: infection 20 m...

文献信息：Perez-Tornero, O.,Porras, I.等.  发表于【Plant Cell, Tissue and Organ Culture】 |  , 请登录

简介：The 27 lemon cultivars analysed could be considered slightly or moderately polyembryonic, with 25 to 43% of seeds being polyembryonic and from 1.3 to 1.6 embryos per seed. On this basis, it is necessary to rescue zygotic embryos at an immature stage. Rescue and in vitro embryo development have been studied in two Citrus limon polyembryonic cultivars. Sucrose (50 and 70 g/l) was combined with Muras...

文献信息：Chen, C. L.,Guo, W. W.,Yi, H. L.,Deng, X. X.等.  发表于【Plant Breeding】 |  , 请登录

简介：Microsporogenesis of two interspecific allotetraploid somatic hybrids, i.e. 'Hamlin' sweet orange + 'Rough Lemon' (HR) and 'Key' lime + 'Valencia' sweet orange (KV), was examined and compared with their corresponding diploid fusion parents. Meiotic analysis revealed that only 13.2 and 18.0% of the meiocytes exhibited normal tetrad formation in HR and KV somatic hybrid plants, respectively, and irr...

文献信息：金莉等.   , 请登录

简介：野生种往往具有常规栽培种少有的优良特性,它们在增加遗传多样性、品种改良等方面都有重要的价值。然而,柑橘的多胚性、童期长、雌/雄性器官败育等特性给野生种的有效利用增加了难度。体细胞杂交技术能有效克服这些问题,实现远缘杂交,使亲本的核或胞质DNA发生重组,转移近缘野生种的有利性状,为柑橘的遗传改良提供了有效的途径。本研究旨在利用体细胞杂交技术,将柑橘的野生品种与枳橙(Poncitrus trifoliata Raf.×Citrus sinensis Osb...

文献信息：体细胞杂交与柑橘无核化遗传改良等.  发表于【2008年园艺植物染色体倍性操作与遗传改良学术研讨会】 |  , 请登录

简介：果实无核是鲜食柑橘品质优良的重要标志,我国有近一半的柑橘为有核类型,采用细胞工程进行我国特色有核柑橘品种的无核化遗传改良具有重要意义。我们建立了成熟的柑橘原生质体操作技术,获得了30余例种间、属间柑橘体细胞杂种/胞质杂种;将优良体细胞杂种的花粉与我国特色地

文献信息：徐小勇,刘继红等.  发表于【华中农业大学学报】 |  , 请登录

简介：采用CAPS(cleaved amplified polymorphic sequence)分子标记对通过非对称原生质体融合获得的2个组合体细胞杂种植株进行了胞质遗传分析。结果表明,在伏令夏甜橙(Citrus sinensis)+默科特橘橙(C.reticulata×C.sinensis)组合中,分析的2棵植株胞质DNA都来自伏令夏甜橙(供体);在丹西红橘(C.retic-ulata)+佩琦橘柚(C.reticulata×C.paradisi)组合中,分析的3棵植株胞质DNA都来自来源于佩琦橘柚(受体)。

文献信息：徐小勇,刘继红,邓秀新等.  发表于【中国园艺学会第八届青年学术讨论会暨现代园艺论坛】 |  , 请登录

简介：自获得首例柑橘体细胞杂种以来,柑橘原生质体融合取得了较大的进展,研究涉及的类型不断增多,研究的范围也不断扩大,研究的手段也得到了提高。2000年以前绝大多数获得的柑橘体细胞杂种,由于研究手段限制或花费较高仅采用同工酶或RAPD分析来确定其杂种性,而没有分析其核质来源。随着分子标记的增多(如AFLP、SSR和CAPS等),柑橘体细胞杂种核质遗传研究也趋于简单。本研究使用Cleaved amplified polymorphic sequence(CAPS)分子标记对本实...

文献信息：王素青等.   , 请登录

简介：柑橘体细胞杂种作为备选的砧木资源和育种材料,其叶柄的结构特征影响其营养的输导能力。 纽荷尔脐橙是我国赣南地区主栽的脐橙品种,近年来缺硼现象日益严重,缺硼后叶片的表现为:黄化,叶片变厚而脆,叶脉爆裂并木栓化,严重影响了叶片的正常功能;盆栽缺硼纽荷尔脐橙的生长势减弱,根系变少。 本试验采用石蜡切片法对几种柑橘体细胞杂种及其亲本和缺硼纽荷尔脐橙的叶片、缺硼盆栽纽荷尔脐橙根系进行解剖学研究,结果如下: 1.柑橘几种体细...