文献信息:Nishikawa F, Endo T, Shimada T, Fujii H, Shimizu T, Omura M.等. 发表于【Journal of the Japanese Society for Horticultural Science】 | , 请登录
简介:To obtain a peel-specific promoter useful for citrus transgenic research, the 5' flanking region of a citrus dlimonene synthase gene (CitMTSE2), which showed a high degree of expression in the peel of mature fruits, was isolated from the satsuma mandarin (Citrus unshiu Marc.) Bacterial Artificial Chromosome (BAC) library. Promoter activity was characterized by particle bombardment, agroinjection a...
文献信息:Deng W, Luo KM, Li ZG, Yang YW, Hu N, Wu Y.等. 发表于【PLANTA】 | 请登录
文献信息:彭爱红, 彭祝春, 何永睿, 许兰珍, 雷天刚, 刘小丰, 姚利晓, 陈善春.等. 发表于【分子植物育种】 | , 请登录
简介:With the mature axillary buds of citrus excellent variety Shinanui(Citrus Reticulate×Citrus sinensis) severed as the explant,The plantlets which carried the hepatitis a virus capsid protein fusion gene were obtained through Agrobacterium-mediated transformation.In this study,survival in graft of mature axillary buds of shinanui was the highest,up to 90%,when the orange rootstocks were cultured 15...
文献信息:Dutt M, Grosser JW.等. 发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录
文献信息:Tong Z, Tan B, Zhang JC, Hu ZY, Guo WW, Deng XX.等. 发表于【SCIENTIA HORTICULTURAE】 | 请登录
文献信息:Zhang J, Tao N, Xu Q, Zhou W, Cao H, Xu J, Deng X.等. 发表于【Plant Cell Reports】 | , 请登录
简介:Citrus, rich in carotenoids, is the most important fruit crop based on the total annual production. In the carotenoid biosynthesis pathway, phytoene synthase (PSY, EC 2.5.1.32) catalyzes the dimerization of two molecules of geranylgeranyl pyrophosphate (GGPP) to phytoene and has been shown to be a rate-limiting enzyme for the synthesis of carotenoids. In this study, we investigated catalytic activ...
文献信息:Mendes AFD, Cidade L, de Oliveira MLP, Otoni WC, Soares WD, Costa M.等. 发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 | , 请登录
简介:Identification of beta-lactam antibiotics that have negligible effects on plant regeneration is a critical step towards the establishment of a reliable Agrobacterium-mediated transformation protocol for perennial trees. In the present report, we have evaluated the effects of the novel beta-lactam antibiotics meropenem and timentin on plant regeneration of a perennial woody fruit plant, Citrus sine...
文献信息:段艳欣, 范净, 郭文武.等. 发表于【中国农学通报】 | , 请登录
简介:‘Nanfeng’tangerine (Citrus reticulata), favorite for its sweet-fleshed and thin-rind fruit, is one of the oldest citrus cultivars in China. However, the decline of its fruit quality affected its commodity value. In order to explore a rapid and effective breeding programme for cultivar improvement, we performed Agrobacterium-mediated transformation of this cultivar using embryogenic callus induce...
文献信息:Niedz RP, Evens TJ.等. 发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录
文献信息:Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW.等. 发表于【Tree Genetics & Genomes】 | 请登录
简介:Precocious trifoliate orange (Poncirus trifoliata [L.] Raf), an extremely early flowering mutant of P. trifoliata, is an attractive model for functional genomics research in Citrus. A procedure for efficient regeneration and transformation of this genotype was developed by using green fluorescent protein (GFP) gene as visual marker and etiolated stem segments as explants. In vivo monitoring of GFP...
文献信息:Dutt M, Barthe G, Francis M, Graham J, Grosser J.等. 发表于【PHYTOPATHOLOGY】 | 请登录
文献信息:谭彬, 郭文武.等. 发表于【《第二届全国果树分子生物学学术研讨会论文集》】 | 请登录
文献信息:Matsuda N, Ikeda K, Kurosaka M, Takashina T, Isuzugawa K, Endo T, Omura M.等. 发表于【Journal of the Japanese Society for Horticultural Science】 | , 请登录
简介:We transformed the European pear (Pyrus communis L.) ‘La France’ and ‘Ballade’ with the Citrus FLOWERING LOCUS T (CiFT) gene, which induces early flowering. Subsequent DNA blot analysis of the transformed and wild-type plants indicated that the transformed plants carried 1–4 copies of CiFT. Of the 8 transformed ‘La France’ and 7 transgenic ‘Ballade’ plant lines obtained, 7 and 5 lines res...
文献信息:Koca U, Berhow MA, Febres VJ, Champ KI, Carrillo-Mendoza O, Moore GA.等. 发表于【Physiologia Plantarum】 | 请登录
简介:Citrus species accumulate large quantities of flavanone glycosides in their leaves and fruit. The physiological role(s) of these compounds in citrus plants are unknown, but they have been documented to benefit human health upon consumption. Flavanone rutinosides are tasteless, whereas flavanone neohesperidosides, such as naringin, give a bitter taste to fruit and fruit juice products, reducing the...
文献信息:Cervera M, Navarro L, Peña L等. 发表于【Journal of Biotechnology】 | , 请登录
简介:Rapid flowering is crucial to perform functional genomic studies to investigate reproductive biology characteristics and fruit quality-related traits in fruit trees. However, long generation cycles of woody plants considerably delay this evaluation. Through genetic transformation, juvenile periods can be significantly shortened by overexpression of flower meristem-identity genes. Transgenic APETAL...
文献信息:Bowman KD, McCollum G, Niedz R, Stover E, Albrecht U, Stange R.等. 发表于【HORTSCIENCE】 | 请登录
文献信息:Vibhute M, Tiwari R, Tripathi MK, Baghel BS, Tiwari S.等. 发表于【Plant Cell Biotechnology and Molecular Biology】 | 请登录
文献信息:Dutt, Manjul Orbovi, Vladimir Grosser, Jude W.等. 发表于【Proceedings of the ... annual meeting of the Florida State H】 | 请登录
文献信息:Endo T, Shimada T, Fujii H, Nishikawa F, Sugiyama A, Nakano M, Shimizu T, Kobayashi Y, Araki T, Peña L, Omura M.等. 发表于【Journal of the Japanese Society for Horticultural Science】 | , 请登录
简介:We have developed a CiFT co-expression system for the rapid evaluation of transgenic citrus flowers and fruit. In order to co-express a target gene and the CiFT gene, a CiFT co-expression vector was constructed by inserting the fragment containing a chimeric target gene into the binary vector harboring P35S::CiFT. This system was applied to metabolically engineer aroma components in trifoliate ora...
文献信息:付行政, 陈传武, 王引, 刘继红等. 发表于【《第二届全国果树分子生物学学术研讨会论文集》】 | , 请登录