文献信息：杨莉等.  请登录

简介：金柑(Fortunella crassifolia Single)为芸香科柑桔亚科金柑属植物，原产中国。与其他柑桔类植物相比，金柑童期较短，实生繁殖后约2-3年便可开花结实，是用作研究果实性状或利用转基因体系研究基因功能的较理想的多年生木本植物材料。 本研究以我国金柑主栽品种‘金弹'为试材，建立了高效的再生体系、遗传转化体系和检测技术体系，获得了转BCH反义基因及叶绿素酶基因启动子的转基因植株，并对转基因材料进行了分子鉴定。主要研究结果...

文献信息：瞿金旺等.  请登录

简介：柑橘是世界上具有重要经济价值的大宗水果。在柑橘的生产过程中，由于冷害、冻害、盐害等环境胁迫的影响，其果实的产量和品质都会明显的下降，人工栽培管理虽然在一定的程度上减少这些伤害，但成效有限。通过育种手段，选育能抵御环境胁迫的优质新品种，是解决这一问题的根本途径。柑橘的遗传转化以其自身的优势，为柑橘新品种育种提供了一条新的途径。 本研究以9个品种的柑橘胚性愈伤及会钱橘、冰糖橙上胚轴切段为试材，采用根癌农...

文献信息：彭爱红等.  请登录

简介： 柑桔是世界上最重要的果树树种之一，目前全世界柑桔种植面积1亿余亩，年总产1亿吨以上，柑桔年贸易额近70亿美元。柑桔也是我国最大宗的水果，2004年栽培面积2441万亩，居世界首位，总产量1496万吨，仅次于巴西、美国居第三位，产值150余亿元。柑桔业现已成为我国南方广大山区农村经济支柱产业之一，在农民致富奔小康、山区水土保持和三峡库区移民安置中发挥了重要作用。
世界各国在发展柑桔生产的同时，也始终注重柑桔品种的质量...

文献信息：唐艳梅等.  请登录

简介：低温冻害是一种常见的环境胁迫因子，也是许多农作物区域性和季节性的限定因素，因此提高植物的抗寒性，一直是科学工作者的理想与追求，抗寒基因的分离、鉴定、克隆及转化一直是国内外科学家研究的热点之一，目前随着植物寒害机理、抗寒冻和冷驯化分子机理的深入发展，已研究发现了多种抗寒基因，包括各种抗寒调控基因和各种抗寒功能基因。常规育种方法很难提高作物的抗冻性，从而促使了植物抗寒冻基因工程的研究得到了广泛开展，国...

文献信息：段艳欣等.  请登录

简介：柑橘是世界上最重要的常绿果树之一，其种植面积和总产量均居水果之首。长期以来，运用传统的育种手段进行柑橘遗传改良受到珠心胚干扰、性器官败育、育种周期长以及遗传上高度杂合等因素的影响，导致育种进程缓慢。转基因技术自1983年应用到植物育种以来，发展迅猛，已经成为基因功能鉴定不可缺少的手段，也是实现植物目标性状改良的一种快速和直接的途径。植物童期长短受开花基因调控，将开花调节基因转入植物有望改变童期。本研究...

文献信息：Carneiro CEA, Molinari HBC, Andrade GA, Pereira LFP, Vieira LGE.等.  发表于【Pesquisa Agropecuaria Brasileira】 | 请登录

简介：The objective of this work was to evaluate the susceptibility to glufosinate ammonium of transgenic plants of Swingle citrumelo with high proline production. The mutant gene of the enzyme Delta(1)-pyrroline-5-carboxylate synthetase (P5CS), the rate-limiting enzyme in proline biosynthesis, was inserted into Swingle citrumelo plants. The expression of the gene p5cs caused up to 5-fold increase on th...

文献信息：Mitani N, Kobayashi S, Nishizawa Y, Kuniga T, Matsumoto R.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：RCC2, cDNA clone encoding rice class-I chitinase, was introduced into trifoliate orange (Poncirus trifoliata Raf.). Chitinase activity of the transformed plants was higher than that of the non-transformed plants. The introduced gene was not detected in the scions grafted onto the transformed plants. The effect of the introduction of foreign gene into rootstocks on the scions was discussed.

文献信息：Kayim M, Koc NK.等.  发表于【SCIENTIA HORTICULTURAE】 | 请登录

文献信息：Azevedo FA, Mourão Filho FAA,Schinor EH, Paoli LG, Mendes BMJ, Harakava R, Gabriel DW, Lee RF.等.  发表于【Pesquisa Agropecuária Brasileira】 | 请登录

简介：The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneratio...

文献信息：Febres V, Lee RF, Moore GA.等.  发表于【PHYTOPATHOLOGY】 | 请登录

文献信息：de Almeida WAB, Mourao FDA, Mendes BMJ, Rodriguez APM.等.  发表于【BIOLOGIA PLANTARUM】 | 请登录

文献信息：胡春华, 谢玉明, 黄训才, 郭琛, 焦徕, 吴芯茹, 邓子牛.等.  发表于【果树学报】 |  , 请登录

简介：In vitro propagation makes it possible to speed the multiplication of the rol A，B，C transgenic citrange plants，SO it is possible to quickly evaluate this new rootstock．Using bud sticks of the transgenic clones(B，D，E)and the control citrange as explants，culture media containing various plant growth regulators were tested for bud bumt，shoot multiplication and rooting．The results indicated t...

文献信息：Roy G, Sudarshana MR, Ullman DE, Ding SW, Dandekar AM, Falk BW.等.  发表于【PHYTOPATHOLOGY】 | 请登录

文献信息：Batuman O, Mawassi M, Bar-Joseph M.等.  发表于【Virus Genes】 | 请登录

简介：In an attempt to utilize post-transcriptional gene silencing (PTGS) as a means to impart resistance against Citrus tristeza virus (CTV) into citrus plants, the p23 + 3?UTR sequence (p23U) of the VT strain of CTV was engineered to fold into a double-stranded (ds) RNA structure. The resulting construct (p23UI) was introduced into Nicotiana benthamiana and Alemow (Citrus macrophylla) plants by Agroba...

文献信息：Fagoaga C, López C, de Mendoza AH, Moreno P, Navarro L, Flores R, Peña L.等.  发表于【Plant Molecular Biology】 |  , 请登录

简介：Previously, we have shown that most Mexican limes (Citrus aurantifolia (Christ.) Swing.) expressing the p23 gene of Citrus tristeza virus (CTV) exhibit aberrations resembling viral leaf symptoms. Here we report that five independent transgenic lines having normal phenotype displayed characteristics typical of post-transcriptional gene silencing (PTGS): multiple copies of the transgene, low levels ...

文献信息：Tao NG, Ye JL, Xu J, Deng XX.等.  发表于【Agricultural Sciences in China】 |  , 请登录

简介：LEA5 gene was postulated related with both stress and hormone responses．In an attempt to find genes exclusively expressed during fruit ripening of Cara Cara navel orange，a novel cDNA clone encoding late embryogenesis—abundant protein 5 like gene(CitLEA5-1)was obtained．It was 582 bp in length，containing 97 deduced amino acids．Compared with the stress．induced LEA5 from leaves of Citrus sinens...

文献信息：Garcia-Luis A, Molina RV, Varona V, Castello S, Guardiola JL.等.  发表于【PLANT CELL TISSUE AND ORGAN CULTURE】 | 请登录

文献信息：Ballester A, Cervera M, Peña L.等.  发表于【Plant Cell Reports】 | 请登录

简介：The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptII, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), w...

文献信息：Omar AA, Song WY, Graham JH, Grosser JW.等.  发表于【HORTSCIENCE】 | 请登录

文献信息：Boscariol RL, Monteiro M, Takahashi EK, Chabregas SM, Vieira ML, Vieira LG, Pereira LF, Mendes BM.等.  发表于【J. Amer. Soc. Hort. Sci.】 |  , 请登录

简介：Citrus canker, caused by Xanthomonas axonopodis Starr and Garces pv. citri(Hasse) Vauterin et al. , is one of the main problems affecting citrus production. In order to obtain resistance to phytopathogenic bacteria, insect gege, coding for antimicrobial proteins, have been used in plant genetic transfornation. In this study, transgenic Citrus sinensis (L.) Osb. "Hamlin" plants expressing the antim...