文献信息：严佳文等.  请登录

简介：柑橘溃疡病(citrus bacterial canker disease)是一种世界检疫性病害，对柑橘产量及品质影响极大，严重危害着柑橘产业的健康发展。胡春华等利用病原基因诱导植物获得抗性原理，将致病基因pthA的核定位信号序列构建正义(pthA-nls)和反义(pthA-a-nls)基因，导入柑橘感病品种甜橙，并已获得转pthA基因甜橙株系。本试验旨在对获得的转化苗进行全面的分子鉴定，明确各株系中目的基因的整合情况和具体拷贝数，并证实目的基因可以正常表达，...

文献信息：覃磊 等.  请登录

简介：柑橘是一种重要的亚热带果树，是全世界重要的经济作物之一。柑橘在生产过程中常常受干旱、低温、病害等各种逆境胁迫的影响。导致果实品质下降，产量降低，农药使用量激增。严重时可造成植株死亡，从而造成了巨大的环境破坏和经济损失。 糖橙(Citrus sinensis L. Osbeck)果形漂亮，果肉橙黄色，可溶性固形物、含糖量高，含酸量低。深受中国消费者的青睐。本试验选取转TERF1糖橙的三个株系及其对照为试验材料，对其分别进行干旱、低...

文献信息：范净等.  请登录

简介：转基因技术应用于改良农作物的特定性状，如耐除草剂、抗病性、抗虫性、抗环境胁迫、提高产量、改善品质等。还可以通过转基因植株超量或抑制特定基因的表达进行目的基因的功能验证。转基因植株能否广泛应用，其关键在于所转基因能否在遗传转化体内稳定整合和表达。本研究以转GFP、AP1、LFY柑橘为材料，分析外源基因的拷贝数、T-DNA整合、甲基化和外源基因表达之间的相关性；采用Real-time PCR在转基因桠柑中筛选单拷贝的转基因植株，...

文献信息：李琳洁 等.  请登录

简介：植物转基因研究中抗性标记基因可以提高转化体的筛选效率。然而，如今可利用的标记基因种类非常的有限，不利于对转基因植物的长期研究。而且人们也对转基因植物的安全性报以怀疑，阻碍了转基因植物研究的发展。在剔除标记基因策略中，可诱导的自主剔除途径显现出较好的利用前景。柑橘溃疡病是一种世界性的检疫性病害，对产业危害极大，因缺乏抗病资源和有效防控方法，该病仍无法根除。pthA-NLS基因具有抑制病原菌致病因子的功能，将...

文献信息：李芳, 戴素明, 杨莉, 邓子牛.等.  发表于【中国园艺学会2010年学术年会】 | 请登录

简介：柑橘衰退病(citrus tristeza virus，CTV)是世界性的柑橘病害，对我国柑橘产业的危害越来越严重。p23基因是柑橘衰退病的沉默抑制子之，在感病植株内的积累与感病程度相关。本研究拟利用p23基因，通过RNAi途径获得抗柑橘衰退病的新种质。
以柑橘衰退病强毒株的p23基因为模板，设计引物构建用于RNAi的dsRNA。通过PCR扩增第18439-18951bp 处的一段527bp片段（引入BamHⅠ-XbaⅠ酶切位点）以及第18661-18951bp处的一段305bp的片段（引入...

文献信息：Silva RP, Souza AJ, Mendes BMJ, Mourão Filho FAA.等.  发表于【Rev. Bras. Frutic., Jaboticabal - SP,】 |  , 请登录

简介：In order to evaluate the formation of adventitious buds and in vitro regeneration of sour orange plants (Citrus aurantium L.) two organogenesis-inducing experiments were conducted. In the first experiment, the induction and in vitro regeneration of adventitious buds were tested on epicotyl and internodal segments under the infl uence of BAP or KIN associated with NAA. The second experiment evaluat...

文献信息：Marutani-Hert M, Evens TJ, McCollum GT, Niedz RP.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：Bud emergence and shoot growth from adult phase citrus nodal cultures were studied using Citrus mitis (calamondin), Citrus paradisi (grapefruit), and Citrus sinensis (sweet orange). The effects of 6-benzyladenine (BA), indole 3-acetic acid (IAA), and citrus type on shoot quality and growth of mature bud explants from greenhouse grown trees were determined using a 2-component mixtureamount 9 citrus...

文献信息：李琳洁, 杨莉, 李芳, 谢玉明, 曾柏全, 邓子牛.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：The marker-free transgenic Citrus was investigated by combining application of a chemical regulated induction system XVE with, Cre/loxP-mediated site-specific DNA recombination in a single transformation to remove carriers. the system can remove marker gene (nptⅡ) by expression of Cre recombinase system, which was tightly controlled by a system upon induction by β-estradiol. The infected interno...

文献信息：Oliveira MLP, Costa MGC, Silva CV, Otoni WC.等.  发表于【Pesquisa Agropecuária Brasileira】 |  , 请登录

简介：The objective of this study was to evaluate the effects of 6?benzylaminopurine (BAP) and α?naphthaleneacetic acid (NAA) combinations, basal media and beta?lactam antibiotics on in vitro organogenesis from mature stem segments of 'Pêra', 'Valência' and 'Bahia' sweet oranges and 'Cravo' rangpur lime. For induction of shoot regeneration, the segments of the four cultivars were placed on Murashige ...

文献信息：Stover E, Stange R, McCollum G, Jaynes J.等.  发表于【HORTSCIENCE】 | 请登录

文献信息：袁飞荣, 李芳, 蒋巧巧, Gentile A, 严佳文, 邓子牛.等.  发表于【中国农学通报】 |  , 请登录

简介：To examine the gene-gene interactions between GA20ox1 and rol gene so as to elucidate the molecular mechanism of dwarfing morphogenesis in citrange.The potted seedlings of transgenic citrange with rol ABC genes were used as trial materials, the sensitive response to GAs was tested by spraying GA3 regularly, the expression of GA20ox1 and rol A,B and C genes were detected by quantitative reverse tra...

文献信息：Dutt M, Lee DH, Grosser JW.等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 |  , 请登录

简介：Agrobacterium-mediated transformation of Carrizo citrange [Citrus sinensis (L.) Osbeck × Poncirus trifoliata (L.) Raf.] with a binary vector containing a novel bifunctional reporter–selection fusion gene comprising an inframe fusion between the manA gene and egfp gene is detailed. This system combined the phosphomannose isomerase positive selection system with the ability to monitor gene express...

文献信息：Dutt M, Mondal SN, Barthe GA, Dewdney MM, Grosser JW.等.  发表于【HORTSCIENCE】 | 请登录

文献信息：李琳洁, 杨莉, 李芳, 谢玉明, 曾柏全, 邓子牛.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：The marker-free transgenic Citrus was investigated by combining application of a chemical regulated induction system XVE with,Cre/loxP-mediated site-specific DNA recombination in a single transformation to remove carriers.The system can remove marker gene(nptⅡ)by expression of Cre recombinase system,which was tightly controlled by a system upon induction byβ-estradiol.The infected internodal ste...

文献信息：Yang L, Hu C, Li N, Zhang J, Yan J, Deng Z.等.  发表于【Plant Molecular Biology】 |  , 请登录

简介：The COOH terminal of pthA encoding three nuclear localizing signals (NLS) was amplified by polymerase chain reaction (PCR) from the plasmid of Xanthomonas axonopodis pv. citri, the pathogen of citrus canker disease. Then the sense and antisense strands of the nls were cloned into pBI121 vector. pthA-nls driven by the CaMV35 s promoter was transferred into sweet orange via Agrobacterium -mediated t...

文献信息：Soler N, Fagoaga C, Lopez C, Moreno P, Navarro L, Flores R , Pena L.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

文献信息：Dutt M, J Madhavaraj, JW Grosser.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Agrobacterium-mediated genetic transformation of a tetraploid ‘‘tetrazyg’’ citrus rootstock selection‘Orange #16’ [Nova mandarin (Citrus reticulata Blanco) + Hirado Buntan pummelo (Citrus grandis L. Osbeck)]  [Cleopatra mandarin (C. reticulata Blanco) + Argentine trifoliate orange (Poncirus trifoliata (L.) Raf.)] was performed. Juvenile epicotyl segments were transformed with a construct co...

文献信息：胡新喜, 袁飞荣, 邓子牛, 甘德欣, 熊兴耀.等.  发表于【热带作物学报】 |  , 请登录

简介：Citranges transferred with phyB gene were detected at the molecular level and their biological characteristics were analyzed.PCR,RT-PCT and PCR-Southern blot analysis indicated that phyB gene was successfully integrated into citrange genome and was transcribed in citrange.Compared with the non-transgenic plant,the transgenic plant was dwarf,with larger shoot insertion angle and smaller leaf area,a...

文献信息：Li ZM, Zhang JZ, Mei L, Deng XX, Hu CG, Yao JL.等.  发表于【PLANT MOLECULAR BIOLOGY】 | 请登录

文献信息：Xu SX, Cai XD, Tan B, Guo WW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：The green fluorescent protein (GFP) has become an ideal visual marker to monitor and quantify the expression of the transgene. It can be targeted to specific subcellular locations, including the endoplasmic reticulum, mitochondria, actin cytoskeleton and nuclei through the addition of signal peptides. Our previous work has resulted in transgenic citrus plants expressing cytoplasmic targeted GFP (C...