文献信息：Oliveira MLP, Costa MGC, Silva CV, Otoni WC.等.  发表于【Pesquisa Agropecuária Brasileira】 |  , 请登录

简介：The objective of this study was to evaluate the effects of 6?benzylaminopurine (BAP) and α?naphthaleneacetic acid (NAA) combinations, basal media and beta?lactam antibiotics on in vitro organogenesis from mature stem segments of 'Pêra', 'Valência' and 'Bahia' sweet oranges and 'Cravo' rangpur lime. For induction of shoot regeneration, the segments of the four cultivars were placed on Murashige ...

文献信息：Stover E, Stange R, McCollum G, Jaynes J.等.  发表于【HORTSCIENCE】 | 请登录

文献信息：袁飞荣, 李芳, 蒋巧巧, Gentile A, 严佳文, 邓子牛.等.  发表于【中国农学通报】 |  , 请登录

简介：To examine the gene-gene interactions between GA20ox1 and rol gene so as to elucidate the molecular mechanism of dwarfing morphogenesis in citrange.The potted seedlings of transgenic citrange with rol ABC genes were used as trial materials, the sensitive response to GAs was tested by spraying GA3 regularly, the expression of GA20ox1 and rol A,B and C genes were detected by quantitative reverse tra...

文献信息：Dutt M, Lee DH, Grosser JW.等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 |  , 请登录

简介：Agrobacterium-mediated transformation of Carrizo citrange [Citrus sinensis (L.) Osbeck × Poncirus trifoliata (L.) Raf.] with a binary vector containing a novel bifunctional reporter–selection fusion gene comprising an inframe fusion between the manA gene and egfp gene is detailed. This system combined the phosphomannose isomerase positive selection system with the ability to monitor gene express...

文献信息：Dutt M, Mondal SN, Barthe GA, Dewdney MM, Grosser JW.等.  发表于【HORTSCIENCE】 | 请登录

文献信息：李琳洁, 杨莉, 李芳, 谢玉明, 曾柏全, 邓子牛.等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：The marker-free transgenic Citrus was investigated by combining application of a chemical regulated induction system XVE with,Cre/loxP-mediated site-specific DNA recombination in a single transformation to remove carriers.The system can remove marker gene(nptⅡ)by expression of Cre recombinase system,which was tightly controlled by a system upon induction byβ-estradiol.The infected internodal ste...

文献信息：Yang L, Hu C, Li N, Zhang J, Yan J, Deng Z.等.  发表于【Plant Molecular Biology】 |  , 请登录

简介：The COOH terminal of pthA encoding three nuclear localizing signals (NLS) was amplified by polymerase chain reaction (PCR) from the plasmid of Xanthomonas axonopodis pv. citri, the pathogen of citrus canker disease. Then the sense and antisense strands of the nls were cloned into pBI121 vector. pthA-nls driven by the CaMV35 s promoter was transferred into sweet orange via Agrobacterium -mediated t...

文献信息：Soler N, Fagoaga C, Lopez C, Moreno P, Navarro L, Flores R , Pena L.等.  发表于【IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL】 | 请登录

文献信息：Dutt M, J Madhavaraj, JW Grosser.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Agrobacterium-mediated genetic transformation of a tetraploid ‘‘tetrazyg’’ citrus rootstock selection‘Orange #16’ [Nova mandarin (Citrus reticulata Blanco) + Hirado Buntan pummelo (Citrus grandis L. Osbeck)]  [Cleopatra mandarin (C. reticulata Blanco) + Argentine trifoliate orange (Poncirus trifoliata (L.) Raf.)] was performed. Juvenile epicotyl segments were transformed with a construct co...

文献信息：胡新喜, 袁飞荣, 邓子牛, 甘德欣, 熊兴耀.等.  发表于【热带作物学报】 |  , 请登录

简介：Citranges transferred with phyB gene were detected at the molecular level and their biological characteristics were analyzed.PCR,RT-PCT and PCR-Southern blot analysis indicated that phyB gene was successfully integrated into citrange genome and was transcribed in citrange.Compared with the non-transgenic plant,the transgenic plant was dwarf,with larger shoot insertion angle and smaller leaf area,a...

文献信息：Li ZM, Zhang JZ, Mei L, Deng XX, Hu CG, Yao JL.等.  发表于【PLANT MOLECULAR BIOLOGY】 | 请登录

文献信息：Xu SX, Cai XD, Tan B, Guo WW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：The green fluorescent protein (GFP) has become an ideal visual marker to monitor and quantify the expression of the transgene. It can be targeted to specific subcellular locations, including the endoplasmic reticulum, mitochondria, actin cytoskeleton and nuclei through the addition of signal peptides. Our previous work has resulted in transgenic citrus plants expressing cytoplasmic targeted GFP (C...

文献信息：Nishikawa F, Endo T, Shimada T, Fujii H, Shimizu T, Kobayashi Y, Araki T, Omura M.等.  发表于【Tree Physiology】 |  , 请登录

简介：Ectopic expression of a Flowering Locus T (CiFT) from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.). To profile transcriptional effects of CiFT, mRNA extracted from the shoots of the transgenic trifoliate orange was subjected to genetic analysis using a 22-K oligo DNA microarray. Microarray results and reverse transcription polymerase chain reaction...

文献信息：覃 磊, 袁飞荣, 曹雄军, 罗赛男, 邓子牛.等.  发表于【中国园艺文摘】 |  , 请登录

简介：The effect of drought stress on the transgenic sweet orange with TERF1 and wild type were researched. The results showed that the leaves of wild type were wilting with the increase of drought stress and the ability of photosynthesis was declining. Until severe drought stress (SRWC=10%), leaves shed and growth tends stop with the chlorophyll content decreased to 50.0%. While the leaves of transgeni...

文献信息：Singh S, Rajam MV.等.  发表于【JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY】 | 请登录

文献信息：Zhang X, Francis MI, Dawson WO, Graham JH, Orbović V, Triplett EW, Mou Z.等.  发表于【European Journal of Plant Pathology】 |  , 请登录

简介：Citrus canker, caused by the bacterial pathogen Xanthomonas citri subsp. citri (Xcc), is a serious leaf and fruit spotting disease affecting many important citrus cultivars including grapefruit and certain sweet oranges. Currently, efficacious and economical disease control measures for highly susceptible citrus cultivars are lacking. Development of commercial cultivars with greater field resistan...

文献信息：Dutt M, Grosser JW.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：A method for the genetic transformation of several citrus cultivars is described, including cultivars observed to be recalcitrant to conventional epicotyl-mediated transformation. Embryogenic cell suspension cultures, established from unfertilized ovules were used as target tissues for Agrobacterium-mediated transformation. Several modifications were made to the culture environment to investigate ...

文献信息：Cervera M, Esteban O, Gil M, Gorris MT, Martínez MC, Peña L, Cambra M.等.  发表于【Transgenic Research】 |  , 请登录

简介：Citrus tristeza virus (CTV) causes one of the most destructive viral diseases of citrus worldwide. Generation of resistant citrus genotypes through genetic engineering could be a good alternative to control CTV. To study whether production of single-chain variable fragment (scFv) antibodies in citrus could interfere and immunomodulate CTV infection, transgenic Mexican lime plants expressing two di...

文献信息：Cardoso SC, Barbosa-Mendes JM, Boscariol-Camargo RL, Christiano RSC, Filho AB, Vieira MLC, Mendes BMJ, Mourão Filho FAA.等.  发表于【Plant Molecular Biology Reporter】 |  , 请登录

简介：Genetic transformation with genes that code for antimicrobial peptides has been an important strategy used to control bacterial diseases in fruit crops, including apples, pears, and citrus. Asian citrus canker (ACC) caused by Xanthomonas citri subsp. citri Schaad et al. (Xcc) is a very destructive disease, which affects the citrus industry in most citrus-producing areas of the world. Here, we repo...

文献信息：胡新喜, 敖小平, 邓子牛, 熊兴耀.等.  发表于【中国农学通报】 |  , 请登录

简介：Transgenic plants appear to be a new way to citrus improvement and a powerful tool for studying gene functions. The establishment of an efficient in vitro plant regeneration system suitable for genetic transformation is the first step in this approach. Different explants of seedlings were grownin vitro and a efficient regeneration system was established for Citrus grandis Osbeck cv. Shatianyou. Th...