文献信息：Rattanpal HS, Kaur G, Gupta M.等.  发表于【African Journal of Biotechnology】 | 请登录

简介：This study was aimed to optimize the organogenesis in rough lemon (Citrus jambhiri Lush), an important citrus rootstock of India. Organogenesis was induced in epicotyl segments of rough lemon seedlings. Three important factors influencing organogenesis in vitro viz. hormone combination, cut modes and photoperiod were evaluated. Etiolation of seedlings had a positive effect on bud formation and hig...

文献信息：Reyes CA, Francesco AD, Peña EJ, Costa N, Plata MI, Sendin L, Castagnaro AP, García ML.等.  发表于【Journal of Biotechnology】 |  , 请登录

简介：The lack of naturally occurring resistance to Citrus psorosis virus (CPsV) has demanded exploitation of a transgenic approach for the development of CPsV-resistant sweet orange plants. Transgenic sweet orange plants producing intron-hairpin RNA transcripts (ihpRNA) corresponding to viral cp, 54K or 24K genes were generated and analyzed at the molecular and phenotypic levels. Two independent CPsV c...

文献信息：Bassan MM, Mourao FDA, Miyata LY, Mendes BMJ.等.  发表于【Pesquisa Agropecuaria Brasileira】 | 请登录

文献信息：Bachchu MAA, Jin SB, Park JW, Sun HJ, Yun SH, Lee HY, Lee DS, Hong QC, Kim YW, Riu KZ, Kim JH.等.  发表于【Horticulture, Environment, and Biotechnology】 |  , 请登录

简介：Agrobacterium-mediated transformation in Satsuma mandarin (Citrus unshiu Marc.) cv. Miyagawa wase was achieved with reasonable transformation efficiency of about 22%, which was the percentage of transgenic plantlets confirmed by genomic PCR (37 plantlets/168 hygromycin-resistant calli). Embryogenic calli of Miyagawa wase were infected with Agrobacterium tumefaciens strain EHA105 harboring binary v...

文献信息：Al Bachchu MA, Jin SB, Park JW, Boo KH, Sun HJ, Kim YW, Lee HY, Riu KZ, Kim JH.等.  发表于【Journal of the Korean Society for Applied Biological Chemistry】 | 请登录

简介：Miraculin is a taste modifying plant protein, which displays a peculiar property of being able to modify sour taste into sweet taste. Thus, there has been an increasing interest in miraculin due to this amazing property. In the present study, miraculin gene was introduced into the Miyagawa Wase Satsuma mandarin (Citrus unshiu Marc.) callus by Agrobacterum-mediated transformation to produce a citru...

文献信息：Pons E, Navarro A, Ollitrault P, Peña L.等.  发表于【PLoS ONE】 |  , 请登录

简介：Background/Objective: Despite potential benefits granted by genetically modified (GM) fruit trees, their release and commercialization raises concerns about their potential environmental impact, and the transfer via pollen of transgenes to cross-compatible cultivars is deemed to be the greatest source for environmental exposure. Information compiled from field trials on GM trees is essential to pr...

文献信息：Orbović V, Soria P, Moore GA, Grosser JW.等.  发表于【Crop Protection】 |  , 请登录

简介：The T36 strain of citrus tristeza virus (CTV) expressing green fluorescent protein gene (CTVeGFP) was used to detect replication of CTV in wild-type (WT) ‘Hamlin’ sweet orange plants and those transformed with the use of Agrobacterium tumefaciens strain harboring a binary vector with a coat protein gene from either T36 or T30 isolate of CTV. Soil-adapted WT and transgenic plants were challenged ...

文献信息：Orbovic V, Dutt M, Grosser JW.等.  发表于【SCIENTIA HORTICULTURAE】 | 请登录

文献信息：Niedz RP, Evens TJ.等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 | 请登录

简介：The objective of this study was to improve shoot regeneration from grapefruit. Because many commercially grown citrus types are apomictic, important in vitro applications such as Agrobacterium-mediated transformation commonly use epicotyl explants from in vitro seedlings; thus, adequate adventitious shoot production is an important prerequisite for efficient use of these applications. Eight plant ...

文献信息：Miyata LY, Mourão Filho FAA, Scarpare Filho JA, Zambon F, Bassan MM, Mendes BMJ, Harakava R.等.  发表于【Revista Brasileira de Fruticultura】 |  , 请登录

简介：Genetic transformation is considered an important tool to be applied in citrus genetic improvement. However, transformation efficiency may vary according to several factors, including the genetic construction utilized. This study was carried out to evaluate the efficiency of genetic transformation of ‘Carrizo’ citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck], containing two...

文献信息：Fu XZ, Chen CW, Wang Y, Liu JH, Moriguchi T.等.  发表于【BMC Plant Biology】 | 请登录

简介：Background: Enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene.
Results: In the present work, an apple spermidine synthase gene (MdSPDS1) was introduced into sweet orange (Citrus sinensis Osbeck ‘Anliucheng’) via Agr...

文献信息：Fu XZ, Khan EU, Hu SS, Fan QJ, Liu JH.等.  发表于【Environmental and Experimental Botany】 |  , 请登录

简介：Trifoliate orange (Poncirus trifoliata L. Raf.), a rootstock widely used for citrus species, is salt-sensitive. Worldwide, salinity is a major abiotic stress affecting citrus growth and yield. Glycinebetaine (GB) is an important osmoprotectant involved in responses to salt stress. However, current evidence regarding the effect of salt stress on GB accumulation in trifoliate orange is limited, and ...

文献信息：Figueiredo JFL, Römer P, Lahaye T, Graham JH, White FF, Jones JB.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：In this study, we present a method for transient expression of the type III effector AvrGf1 from Xanthomonas citri subsp. citri strain Aw in grapefruit leaves (Citrus paradisi) via Agrobacterium tumefaciens. The coding sequence of avrGf1 was placed under the control of the constitutive CaMV 35S promoter in the binary vectors pGWB2 and pGWB5. Infiltration of grapefruit leaves with A. tumefaciens ca...

文献信息：Fan J, Liu X, Xu SX, Xu Q, Guo WW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：In transgenic plants, multiple T-DNA copies and cytosine methylation usually correlate with a loss of transgene expression while methylation of promoter is associated with transgene silencing. Here, six independent GFP transgenic ‘Bingtang’ sweet orange (Citrus sinensis (L.) Osb.) plants were analyzed with copy number, T-DNA repeat, cytosine methylation and transgene expression. The fluorescence...

文献信息：Qin L, Luo SL, Deng ZN, Yan JW, Li N, Yuan FR.等.  发表于【Acta Horticulturae】 | 请登录

文献信息：Singer SD, Hily JM, Cox KD.等.  发表于【Planta】 | 请登录

文献信息：Dutt M, Grosser JW.等.  发表于【Acta Horticulturae】 | 请登录

文献信息：Dutt M, Vasconcellos M, Grosser JW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：Four antioxidants including glycine betaine, glutathione, lipoic acid, and polyvinylpyrrolidone were evaluated to improve transformation efficiency of Mexican lime, a precocious but recalcitrant citrus cultivar to Agrobacterium mediated transformation. Lipoic acid substantially improved the transformation efficiency of Mexican lime by aiding in callus development and improving shoot growth from cu...

文献信息：He Yongrui, Chen Shanchun, Peng Aihong, Zou Xiuping, Xu Lanzheng, Lei Tiangang, Liu Xiaofeng, Yao LX.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：Transgenic plants of Jincheng orange (Citrus sinensis Osbeck) and Newhall navel orange (C. sinensis Osbeck) containing antibacterial peptide genes Shiva A and Cecropin B were successfully obtained by a novel Agrobacterium tumefaciens-mediated transformation of the mature axillary buds. PCR and Southern blot analysis of the transgenic plants verified that the Shiva A and Cecropin B genes were integ...

文献信息：Khan EU, Fu XZ, Liu JH.等.  发表于【Acta Horticulturae】 | 请登录