文献信息：Khan EU, Fu XZ, Liu JH.等.  发表于【 Plant Cell Tissue and Organ Culture】 | 请登录

简介：In this study, attempts were made to develop a protocol for regeneration of transgenic plants via Agrobacterium tumefaciens-mediated transformation of leaf segments from 'Valencia' sweet orange (Citrus sinensis L. Osbeck) using gfp (green fluorescence protein) as a vital marker. Sensitivity of the leaf segments regeneration to kanamycin was evaluated, which showed that 50 mg l(-1) was the best amo...

文献信息：Favero P, Mourao FDA, Stipp LCL, Mendes BMJ.等.  发表于【Acta Physiologiae Plantarum】 | 请登录

简介：The difficulty in adult tissue genetic transformation in woody species is still an obstacle to be overcome, including in most sweet orange cultivars of the Brazilian citrus industry. This work reports that, after in vitro culture adjustments, transgenic adventitious buds of 'Hamlin', 'Pra', and 'Valencia' sweet oranges (Citrus sinensis L. Osbeck) were recovered using adult material as explant sour...

文献信息：Dutt M, Ananthakrishnan G, Jaromin MK, Brlansky RH, Grosser JW.等.  发表于【Tree Physiology】 | 请登录

简介：'Mexican' lime (Citrus aurantifolia Swingle) was transformed with constructs that contained chimeric promoter-gus gene fusions of phloem-specific rolC promoter of Agrobacterium rhizogenes, Arabidopsis thaliana sucrose-H+ symporter (AtSUC2) gene promoter of Arabidopsis thaliana, rice tungro bacilliform virus (RTBV) promoter and sucrose synthase l (RSs1) gene promoter of Oryza sativa (rice). Histoch...

文献信息：Curtis IS, Mirkov TE.等.  发表于【Plant Cell Tissue and Organ Culture】 | 请登录

简介：The development of a reliable shoot regeneration system for mature tissue of citrus is of major importance to accelerate the evaluation of commercial traits. Three non-ionic surfactants were evaluated independently in terms of their affects on the growth and regeneration of mature internodal stem segments of sweet orange cv. Hamlin in culture. Growth and shoot development of explants were influenc...

文献信息：Cevik B, Lee RF, Niblett CL.等.  发表于【Turkish Journal of Agriculture and Forestry】 | 请登录

简介：Citrus paradisi Macfad. 'Duncan' was transformed with constructs coding for the wild-type and mutant RNA-dependent RNA polymerase (RdRp) of Citrus tristeza virus (CTV) for exploring replicase-mediated pathogen-derived resistance (RM-PDR). The RdRp gene was amplified from a CTV genome and used to generate the wild-type and 2 mutant RdRp constructs for plant transformation. One mutant had the key am...

文献信息：Caruso P, Baldoni E, Mattana M, Paolo DP, Genga A, Coraggio I, Russo G, Picchi V, Recupero GR, Locatelli F.等.  发表于【Plant Cell Tissue and Organ Culture】 | 请登录

简介：Oxygen deficit, which occurs in flooded or poorly drained soils, can limit plant growth and development. Low-oxygen environmental conditions also limit the distribution of many woody plants, such as citrus trees, which are considered flood-sensitive crops, although tolerance to this stress varies among genotypes and rootstocks. In this study, the rice transcription factor Mybleu was inserted into ...

文献信息：Muniz FR, De Souza AJ, Stipp LCL, Schinor E, Freitas W, Harakava R, Stach-Machado DR, Rezende JAM, Mourao FAA, Mendes BMJ.等.  发表于【Biologia Plantarum】 |  , 请登录

简介：Transgenic Citrus sinensis (L.) Osb. plants, cvs. Valencia and Hamlin, expressing Citrus tristeza virus (CTV) derived sequences were obtained by genetic transformation. The gene constructs were pCTV-CP containing the 25 kDa major capsid protein gene (CTV-CP), pCTV-dsCP containing the same CTV-CP gene in an intron-spliced hairpin construct, and pCTV-CS containing a 559 nt conserved region of the CT...

文献信息：MARCOS A. MACHADO , MARIÂNGELA CRISTOFANI-YALY , MARINêS BASTIANEL 等.  发表于【Revista Brasileira De Fruticultura】 |  , 请登录

简介：Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture regarding the phytosanitary problems. A higher number of varieties/genotypes with potential for commercial growing, either for the industry or fresh market, has been one of the main objectives of citrus bree...

文献信息：Dambier D, Benyahia H, Pensabene-Bellavia G, Kacar YA, Froelicher Y, Belfalah Z, Lhou B, Handaji N, Printz B, Morillon R, Yesiloglu T, Navarro L, Oll等.  发表于【Plant Cell Reports】 | 请登录

简介：The prevalence of sour orange rootstock in the southern and eastern part of the Mediterranean Basin is presently threatened by the spread of Citrus Tristeza Virus (CTV) and its main vector Toxoptera citricida, combined with abiotic constraints such as drought, salinity and alkalinity. The search for alternative CTV-resistant rootstocks that also withstand the other constraints is now considered an...

文献信息：Kamiri M, Stift M, Srairi I, Costantino G, Bakry F, Ollitrault P, Froelicher Y, Moussadik ElA, Hmyene A.等.  发表于【Plant Cell Reports】 | 请登录

简介：Artificial tetraploid somatic hybrids have been developed for sterile triploid citrus breeding by sexual hybridization between diploid and tetraploid somatic hybrids. The genetic structure of diploid gametes produced by tetraploid genotypes depends on the mode of chromosome association at meiosis. In order to evaluate tetraploid inheritance in a tetraploid interspecific somatic hybrid between mand...

文献信息：Gulsen O, Uzun A, Seday U, Kafa G.等.  发表于【Scientia Horticulturae】 |  , 请登录

简介：This study was conducted to assess the genetic basis and develop a regression model for a QTL trait, fruit setting of a full sib population of 164 hybrids obtained between 'Clementine' mandarin (Citrus reticulata Blanco 'Clementine) and 'Orlando' tangelo' (Citrus paradisi Macf. 'Duncan' x C. reticulata Blanco 'Dancy'). Fruit setting of a 164 full sib population field-planted in 2007 was evaluated ...

文献信息：谭洪泉,陈善春等.  发表于【安徽农业科学】 |  , 请登录

简介：柑橘的多胚现象是柑橘杂交育种工作中面临的主要问题,胚挽救技术是解决多胚问题的有效方法。介绍了胚挽救技术中胚分离时间、培养基和培养温度等对合子胚发育的影响,并详细介绍了适合柑橘合子胚早期鉴定的分子标记技术(RAPD、AFLP、SSR和SNP等)及它们在早期鉴定中的优势和不足。

文献信息：唐小浪,马培恰,吴文,黄永敬,王平,黄炽文,肖蔓洪,陈国添等.  发表于【热带作物学报】 |  , 请登录

简介：用60Co-γ射线4、6、8 kR辐射‘年桔’秋梢芽条,嫁接成活率分别为68.4%、41.3%和16.5%,对照成活率为93.2%。通过连续2 a的果实调查分析,获得少核性状稳定、综合性状优良的突变体4个,平均单果种子含量3.3～6.6粒,极显著低于对照的17.6粒。少核‘年桔’突变体的花粉生活力在45.5%～68.8%,发芽率在11.8%～16.7%,均分别极显著低于对照的97.3%和54.1%,说明少核‘年桔’少核的主要原因是花粉败育。

文献信息：Marques NT, Nolasco GB, Leitao JP.等.  发表于【Scientia Horticulturae】 | 请登录

简介：bud and shoot regeneration. High rates of bud initiation and shoot development were obtained both with BA supplemented medium, in the range from 1 mg L(-1) to 3 mg L(-1), and with 0.1 mg L(-1) NAA supplemented medium. NAA concentrations above 1 mg L(-1) significantly reduced bud initiation and shoot elongation. The results obtained using different in vitro culture vessels such as Petri dishes, tub...

文献信息：袁飞荣.等.  请登录

简介：柑橘是我国重要的果树,培育矮化砧木和接穗品种一直是柑橘育种的重要目标之一。柑橘天然矮化砧木种质极少,传统育种因面临漫长的童期、高度杂合,以及珠心胚干扰等问题,目前进展缓慢。应用矮化基因,通过基因工程技术将一个或多个基因转入到柑橘基因组中,可望加快柑橘砧木矮化育种的进程。 rol ABC基因克隆于Agrobactecium rhizogenes菌A4的Ri质粒的TL-DNA区,转入rol基因可诱发植株出现节间变短,分枝增多,顶端优势散失,大量毛根形成,植...

文献信息：文露清, 杨莉, 胡威, 邓子牛.等.  发表于【中国园艺文摘】 | 请登录

简介：以金柑实生苗为试材进行试验,研究不同再生培养基和苗龄及不同暗培养时间对金柑不定芽再生的影响,建立一套高效稳定的金柑组织培养再生体系。结果表明:采用35d苗龄的无菌苗上胚轴茎段在MS+6-苄氨基嘌呤(6-BA)2.0mg/L+α-萘乙酸(NAA)1.0mg/L+激动素(KT)1.0mg/L+3%蔗糖+0.8%琼脂(pH值5.7)再生培养基上,不经过暗培养直接转移至光/暗周期16h/8h条件下,为诱导金柑不定芽再生的最佳条件,再生率高达90%。

文献信息：文露清等.  请登录

简介：柑橘是世界上最重要的水果之一。转基因育种是常规育种的重要辅助手段,成为实现目标性状改良的一种快速、有效的途径。但人们担心标记基因的环境和食品等安全性问题。"Gene-Deletor"技术(外源基因清除技术)是有望解决转基因植物环境安全性和食品安全性问题的有力工具。对于柑橘这类水果,种子是不受欢迎的特性,改良这些品种,实现无核化可提高其市场竞争力。因此,本试验从美国康涅狄格大学李义教授实验室引进的‘'Gene-Deletor"技术和果...

文献信息：梅星星等.  请登录

简介：生物和非生物胁迫严重制约了柑橘生产,已有研究表明多胺在植物抗逆性中发挥重要作用,本实验室在前期将枳中克隆的多胺合成相关基因PtADC转入冰糖橙并获得转基因株系,但对其抗性未加以分析。本研究以517、533和576三个转基因株系和未转化植株(WT)为材料,对其生物与非生物逆境抗性进行了评估,主要研究结果如下： 1.内源多胺含量和表达分析：通过HPLC检测多胺含量可知,三个转基因株系的腐胺、亚精胺含量和多胺总量高于WT,并且腐胺在多胺...

文献信息：马鑫.等.  请登录

简介：柠檬苦素类似物是引起柑橘苦味的主要原因之一,柑橘的苦味对柑橘果实的鲜食和加工品质有明显不利影响。此外,柠檬苦素类似物具有抗肿瘤、抗炎、镇痛、昆虫拒食等很多药理活性。由于柠檬苦素类似物具有这些功能,所以柠檬苦素类似物的需求日益增加,但是苦味的存在限制了人们对它的摄取。柠檬苦素类似物配糖体具有水溶性且无苦味等特性,其生物学活性和柠檬苦素类似物是相似的。因而将其作为替代性食品添加剂,有很好的市场前景。 本研究...

文献信息：付行政等.  请登录

简介：柑橘是世界最重要的果树之一,其产业的发展对促进地区经济和人类健康具有重要意义。在过去的十年中,柑橘生产取得了巨大进展,但是生物和非生物胁迫一直是制约柑橘生产的重要因素,其中溃疡病、盐害以及低温对柑橘产业造成的经济损失十分惨重。因此培育抗性强的柑橘品种是解决这些问题的关键。由于传统育种手段在柑橘上存在的局限性,利用基因工程技术将一些重要的抗性基因转入柑橘是获得抗性种质最为快捷高效的途径。已有研究表明,多胺...