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  • 701. CjFRO2在转基因枳中的整合与表达

    文献信息:刘小丰, 彭爱红, 许兰珍, 雷天刚, 姚利晓, 何永睿, 陈善春.等.  发表于【果树学报】 |  , 请登录

    简介:Iron is an essential trace element for plants,and it is well known that iron deficiency is a significant global problem.Fruit trees are perennial plants,have been more seriously affected by iron deficiency.Breeding for tolerance to iron deficiency using conventional breeding and selection techniques is very time-consuming,laborious,and inefficient.Genetically modifying fruit trees to improve i...

    702. Isolation of a citrus promoter specific for reproductive organs and its functional analysis in isolated juice sacs and tomato

    文献信息:Sorkina A, Bardosh G, Liu YZ, Fridman I, Schlizerman L, Zur N, Or E, Goldschmidt EE, Blumwald E, Sadka A.等.  发表于【Plant Cell Reports】 |  , 请登录

    简介:While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was detected in vegetative organs. These expression patterns suggested that Cl111 is...

    703. Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus-derived Resistance against Psorosis A and Psorosis B

    文献信息:Reyes CA, Zanek MC, Velazquez K, Costa N, Plata MI, Garcia ML.等.  发表于【Journal of Phytopathology】 |  , 请登录

    简介:Citrus psorosis is a widespread serious disease of citrus caused by Citrus psorosis virus (CPsV). In Argentina and Uruguay, this disease is spread by an unknown vector and there is no natural resistance or tolerance to the disease. There are two types of psorosis, described according to the symptoms observed in citrus trees, psorosis A (PsA) and psorosis B (PsB). PsA protects against the severe ef...

    704. Citrus tristeza virus infection induces the accumulation of viral small RNAs (21–24-nt) mapping preferentially at the 3\'-terminal region of the genomic RNA and affects the host small RNA profile

    文献信息:Ruiz-Ruiz S, Navarro B, Gisel A, Peña L, Navarro L, Moreno P, Di Serio F, Flores R.等.  发表于【Plant Molecular Biology】 | 请登录

    简介:To get an insight into the host RNA silencing defense induced by Citrus tristeza virus (CTV) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by deep sequencing (Solexa-Illumina) the small RNAs (sRNAs) in three virus-host combinations. Our data show that CTV sRNAs: (i) represent more than 50% of the total sRNAs in Mexican lime...

    705. Evaluation of citrus rootstock transgenic for rolABC genes

    文献信息:La Malfa S, Distefano G, Domina F, Nicolosi E, Toscano V, Gentile A.等.  发表于【Acta Horticulturae】 | 请登录

    706. Progress towards the development of transgenic disease resistance in citrus.

    文献信息:Grosser JW, Dutt M, Omar A, Orbovic V, Barthe G.等.  发表于【Acta Horticulturae】 | 请登录

    707. 用实时定量PCR分析转roZABC基因积橙外源基因表达模式(摘要)

    文献信息:袁飞荣, 严佳文, 罗坤, 王晓君, 邓子牛.等.  请登录

    简介:rolABC genes were cloned from T-DNA of Ri plasmid in Agrobactecium rhizogenes, apical dominance weakened, lateral branehes increased. internode shortened and some other special charaeters induced by transformation these genes into plants. It's very important to understand the molecular process of plant growth and development and charaeter formation, real一time PCR is high efficient and convenient ...

    708. Establishment of a transformation system for precocious trifoliate orange and molecular breeding for potential seedlessness in citrus

    文献信息:Tan B, Guo WW.等.  发表于【Acta Horticulturae】 | 请登录

    709. N-(2-chloro-4-pyridyl)-N-phenylurea (4-CPPU) enhances in vitro direct shoot organogenesis of Citrus aurantium L

    文献信息:Roussosi PA, Dimitriou G, Voloudakis AE.等.  发表于【SPANISH JOURNAL OF AGRICULTURAL RESEARCH,】 | 请登录

    710. Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry

    文献信息:Dambier D, Benyahia H, Pensabene-Bellavia G, Aka Kaçar Y, Froelicher Y, Belfalah Z, Lhou B, Handaji N, Printz B, Morillon R, Yesiloglu T, Navar等.  发表于【Plant Cell Reports】 |  , 请登录

    简介:The prevalence of sour orange rootstock in the southern and eastern part of the Mediterranean Basin is presently threatened by the spread of Citrus Tristeza Virus (CTV) and its main vector Toxoptera citricida, combined with abiotic constraints such as drought, salinity and alkalinity. The search for alternative CTV-resistant rootstocks that also withstand the other constraints is now considered an...

    711. Genetic transformation efficiency of ‘Carriza’ citrange with two gene constructions

    文献信息:Miyata LY, Mourão Filho FAA, Scarpare Filho JA, Zambon F, Bassan MM, Mendes BMJ, Harakava R等.  发表于【Rev. Bras. Frutic., Jaboticabal - SP,】 |  , 请登录

    简介:Genetic transformation is considered an important tool to be applied in citrus genetic improvement. However, transformation efficiency may vary according to several factors, including the genetic construction utilized. This study was carried out to evaluate the efficiency of genetic transformation of ‘Carrizo’ citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck], containing two...

    712. Resistance analysis in grapefruit and mexican lime transformed with the p25 Citrus tristeza virus ge

    文献信息:Loeza-Kuk E, Gutierrez-Espinosa MA, Ochoa-Martinez DL, Villegas-Monter A, Mora-Aguilera G, Palacios-Torres EC, Perez-Molphe-Balch E.等.  发表于【Publicado como ART?CULO en Agrociencia】 |  , 请登录

    简介:In citrus tristeza management tolerant rootstocks have been used, but have less plasticity than C. aurantium, and crossprotection sometimes is overcome by more severe variants. An alternative may be transgenic plants with pathogenderived resistance PDR. To evaluate the conferred PDR with transgenesis plants of Citrus paradisi and C. aurantifolia, transformed via Agrobacterium tumefaciens and A. rh...

    713. Ectopic expression of the p23 silencing suppressor of Citrus tristeza virus differentially modifies

    文献信息:Fagoaga C, Pensabene-Bellavia G, Moreno P, Navarro L, Flores R, Pena L等.  发表于【Molecular Plant Pathology】 |  , 请登录

    简介:Citrus tristeza virus (CTV), a phloem-restricted closterovirus infecting citrus, encodes three different silencing suppressors (p25, p20 and p23), one of which (p23) is a pathogenicity determinant that induces aberrations resembling CTV symptoms when expressed ectopically in transgenic citrus hosts. In this article, the effect of p23 ectopic expression on virus infection was examined in sweet oran...

    714. Creation of transgenic citrus free from limonin bitterness. Biotechnology for improved foods and fl

    文献信息:Hasegawa S, Suhayda C, Omura M, Berhow M等.  发表于【Biotechnology for improved foods and flavors】 | 请登录

    715. 转基因枳橙中rolABC基因荧光定量表达分析方法的建立

    文献信息:袁飞荣, 严佳文, 罗坤, Alessandra ,Gentile, 王晓君, 刘东波, 邓子牛等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

    简介:以转rolABC基因枳橙B、D、E系及野生植株为材料,制备标准品,采用SYBR Green I染料,构建rol基
    <br />因和β-actin基因的双标准曲线,校正引物扩增效率后,用 2-&#8710;&#8710;Ct法对rol基因在嫩茎、嫩叶、功能叶、树皮和根中的mRNA水平进行相对定量,建立适合于rolA、rolB、rolC基因实时荧光定量RT-PCR分析方法.初步认为rolC基因在嫩茎、嫩叶、功能叶和树皮中表达量最高;其次是rolA基因,主要在嫩茎中表达;rolB基因表达量最低...

    716. Cloning, biochemical identification, and expression analysis of a gene encoding S-adenosylmethionine decarboxylase in navel orange (Citrus sinensis Osbeck)

    文献信息:Wang J, Liu JH, Kurosawa T, Nada K, Ban Y, Moriguchi T等.  发表于【Journal of Horticultural Science & Biotechnology】 | 请登录

    简介:Polyamines have been shown to be involved in a variety of plant physiological processes such as growth, development, and stress responses. As S-adenosylmethionine decarboxylase (SAMDC) is the rate-limiting enzyme for polyamine biosynthesis, efforts were made to isolate the gene encoding SAMDC from \'Newhall\' navel orange (Citrus sinensis Osbeck). RT-PCR, in combination with a bioinformatics appr....

    717. Proteomic analysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummelo

    文献信息:Wang L, Pan ZY, Guo WW.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC), 】 |  , 请登录

    简介:In order to predict the performance of growth, development and resistance of a citrus diploid cybrid plant between Citrus unshiu Marc. cv. Guoqing No. 1 (G1) and Hirado Buntan pummelo (Citrus grandis (L.) Osbeck) (HBP), the proteomes of leaves from this cybrid and its parents were investigated. First, the diploid cybrid plant (G1 + HBP) created with its nuclear and chloroplast genomes from HBP and...

    718. Assessment of the origin of new citrus tetraploid hybrids (2n=4x) by means of SSR markers and PCR based dosage effects

    文献信息:Ferrante SP, Lucretti S, Reale S, Patrizio AD, Abbate L, Tusa N, Scarano MT.等.  发表于【Euphytica】 |  , 请登录

    简介:We report the accurate determination of the allelic configurations of a total of eight new citrus tetraploid hybrids by means of SSR analysis, coupled with capillary electrophoresis, and PCR based dosage effects. Tetraploid hybrids were spontaneously obtained from different interploid crosses (2x x 4x) between diploid 'Femminello' lemon and the allotetraploid somatic hybrid (2n = 4x = 36) 'Key' li...

    719. Recovery of citrus triploid hybrids by embryo rescue and flow cytometry from 2x x 2x sexual hybridisation and its application to extensive breeding programs

    文献信息:Aleza P, Juarez J, Cuenca J, Ollitrault P, Navarro L.等.  发表于【Plant Cell Reports】 | 请登录

    简介:Seedlessness is one of the most important characteristics for mandarins for the fresh-fruit market and mandarin triploid hybrids have this trait. Citrus triploid plants can be recovered by 2x x 2x sexual hybridisations as a consequence of the formation of unreduced gametes at low frequency. Triploid embryos are found in small seeds that do not germinate under greenhouse conditions. Extensive breed...

    720. The Mining of Citrus EST-SNP and Its Application in Cultivar Discrimination

    文献信息:Jiang D, Ye QL, Wang FS, Cao L.等.  发表于【Agricultural Sciences in China】 | 请登录

    简介:Single nucleotide polymorphisms (SNPs) are the most abundant sequence variations found in plant genomes and are widely used as molecular genetic markers in cultivar identification and genetic diversity studies. The objective of this study was to identify SNP markers useful for discrimination of citrus cultivars, since large numbers of expressed sequence tags (ESTs) of sweet orange are available fr...

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