文献信息：袁飞荣 袁耀明 李智鑫 Alessandra Gentile 邓子牛等.  2015-12-14 发表于【园艺学报】 |  , 请登录

简介：转rol ABC基因枳橙莲座状分枝、矮化性状突出。为探索其侧枝生长的植物激素调控机制,通过RT-PCR法从枳橙中克隆到独脚金内酯信号转导关键元件CPMAX2基因,分析了该基因在转rol ABC基因枳橙腋生嫩叶中的转录表达,构建了CPMAX2植物过表达载体。研究表明:CPMAX2与甜橙中同源基因一致性为99.66%,编码694个氨基酸,具有一个典型的F-box蛋白结构域和两个LRR重复区,与甜橙MAX2氨基酸序列一致性为99.14%。CPMAX2在转rol ABC基因枳橙3个株系腋生...

文献信息：谢亮亮等.   , 请登录

简介：柑橘童期长达8年以上,严重阻碍了遗传育种进展,因此缩短童期有着十分重要的意义。作为柑橘类唯一的落叶果树,枳/早实枳与大多数落叶的草本植物相似,需要经过冬季低温诱导(春化作用)才能开花。FLOWERING LOCUS C(FLC)是草本植物春化途径关键基因。冬性一年生植物在秋季萌发,在冬季前由于FLC高水平表达导致成花受到抑制,在冬季时由于低温春化作用导致体内FLC的表达量降低,并拥有在春天成花的能力。本实验室前期从枳中分离了FLC同源基因...

文献信息：李秋景等.   , 请登录

简介：柠檬作为世界第一大果树——柑橘的重要的类型,不仅具有很高的食用价值,而且在基础研究方面有着极高的研究价值。利用短童期的种质资源以加快研究进程,一直是柑橘分子生物学研究的重要课题。而短童期的柠檬是柑橘遗传转化的优良材料。本研究利用根癌农杆菌介导法,以早花柠檬和四倍体费米耐劳柠檬上胚轴为外植体,以绿色荧光蛋白基因(GFP)为目的基因,进行遗传转化,最终获得了纯合的GFP稳定表达的阳性苗。主要结果如下:1.早花柠檬上胚轴...

文献信息：解栋梁 邹修平 许兰珍 何丹丹 彭爱红 何永睿 陈善春等.  发表于【中国南方果树】 |  , 请登录

简介：为了研究抗菌肽和人溶菌酶对柑桔溃疡病和黄龙病等的协同抗性,利用连接多肽2A编码序列连接抗菌肽cecropin B(CB)和人溶菌酶human lysozyme(HLy)基因,构建了融合基因CB:HLy及其超量表达载体p35S::CB:HLy。利用农杆菌介导法,将该载体转化至锦橙上胚轴。通过GUS化学染色及PCR鉴定,获得转基因植株32株。Real-time PCR证明了融合基因在转基因锦橙中得到了高效表达。本研究为柑桔溃疡病的抗性研究提供了材料。

文献信息：姜波 钟云 曾继吾 谢文根 吴大清 易干军 闫化学 钟广炎 陈良周 彭成绩 蔡明段等.  2015-09-23 发表于【园艺学报/http：//www. ahs. ac. cn】 |  , 请登录

简介：‘粤丰早橘’是从‘南丰蜜橘’芽变中选育出来的优良新品种,具有适应性强,丰产稳产、果实大、化渣性好等特点,平均单果质量58.33 g,可溶性固形物含量13.36%,总糖含量11.80%,总酸含量0.52%。在广东韶关地区种植,果实10月上旬成熟。

文献信息：Xu, Juan Ma, Lili Jiang, Dong Zhu, Shiping Yan, Fuhua Xie, Yunxia Xie, Zongzhou Guo, Wenwu Deng, Xiuxin等.  2015-08-08 发表于【Food chemistry】 |  , 请登录

简介：Due to the furanocoumarin compounds in the fruit, the production and consumption of grapefruit have
<br />been affected in the past decades since the ‘grapefruit juice effect’ was declared. To provide elite germ-
<br />plasm and obtain knowledge for future citrus breeding programs, the contents of 4 furanocoumarin
<br />monomers (FCMs) in the juice sacs from 73 citrus germplasms were evaluated u...

文献信息：Wu, X. M. Kou, S. J. Liu, Y. L. Fang, Y. N. Xu, Q. Guo, W. W.等.  2015-01-23 发表于【Plant Biotechnol J】 |  , 请登录

简介：Somatic embryogenesis (SE) is a process of somatic cells becoming dedifferentiated and generating embryos. SE has been widely used in biotechnology as a powerful way of regeneration and a model system for studying plant embryogenesis, but the controlling mechanisms of SE are far from clear. Here, we show the genomewide profiles of miRNAs/siRNAs and their target genes in nonembryogenic and embryoge...

文献信息：Soler, N. Fagoaga, C. Lopez, C. Moreno, P. Navarro, L. Flores, R. Pena, L.等.  2014-10-01 发表于【Mol Plant Pathol】 |  , 请登录

简介：Citrus tristeza virus (CTV) is phloem restricted in natural citrus hosts. The 23-kDa protein (p23) encoded by the virus is an RNA silencing suppressor and a pathogenicity determinant. The expression of p23, or its N-terminal 157-amino-acid fragment comprising the zinc finger and flanking basic motifs, driven by the constitutive 35S promoter of cauliflower mosaic virus, induces CTV-like symptoms an...

文献信息：Peng, A. H. Liu, X. F. He, Y. R. Xu, L. Z. Lei, T. G. Yao, L. X. Cao, L. Chen, S. C.等.  2015-03-16 发表于【Biologia Plantarum】 |  , 请登录

简介：Iron deficiency chlorosis occurs frequently in calcareous soils. The transformation of plants with ferric chelate
<br />reductase genes (FROs) provides a potential strategy to alleviate plant chlorosis under iron deficiency. A CjFRO2 gene
<br />isolated from Citrus junos Sieb. ex Tanaka was introduced into Poncirus trifoliata (L.) Raf via Agrobacterium-mediated
<br />transformation. The transgene ...

文献信息：Peng, Aihong Xu, Lanzhen He, Yongrui Lei, Tiangang Yao, Lixiao Chen, Shanchun Zou, Xiuping等.  2015-09-03 发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：Marker-free transgenic plants alleviate con-
<br />cerns regarding the biosafety of genetically modified
<br />organisms and promote their commercialization. In this
<br />study, a transformation vector pLI35SAAT, harboring a
<br />Cre/loxP-mediated recombination system combined with
<br />the isopentenyl transferase (ipt) selectable marker gene and
<br />an anti-bacterial peptide gene AATCB, was ...

文献信息：Nishawy, Elsayed Sun, Xiaohua Ewas, Mohamed Ziaf, Khurram Xu, Rangwei Wang, Dan Amar, Mohamed Zeng, Yunliu Cheng, Yunjiang等.  发表于【Scientia Horticulturae】 | 请登录

简介：Citrus is a perennial woody crop of global economic importance. However, the regulatory mechanism of fruit ripening in citrus remains poorly understood. To explore the regulatory roles of transcription factors in fruit ripening, CgDREB (dehydration responsive element binding) gene was characterized and was functionally overexpressed in tomato (Solanum lycopersicum, cv. Ailsa Craig), resulting in s...

文献信息：Mondal, B Pramanick, S Saha, R Karmakar, M等.  2015-01-18 发表于【International Journal of Biosciences (IJB)/http://dx.doi.org/10.12692/ijb/6.2.128-133】 |  , 请登录

简介：The Polyembryony trait is a characteristic feature of the genus Citrus. This trait is regarded as a nuisance in hybridization but could be effectively utilized for Citrus propagation. The occurrence of nucellar embryony could be regarded as a replacement of costly tissue culture technique and identification of nucellar seedlings of Citrus reticulata may lead to proper management of the elite germp...

文献信息：Kim, In-Jung等.  发表于【Plant and Animal Genome XXIII Conference;Plant and Animal Genome】 | 请登录

简介：A genomic DNA clone encoding carotenoid isomerase (CRTISO) was isolated from Citrus unshiu. It has 12 introns, 13 exons, and 2501 bp of flanking region 5’ of the translational start codon. To identify(X) regulatory elements in the citrus CRTISO (citCRTISO) gene, 2501 bp, 1603 bp and 1037 bp of presumptive promoter DNA sequences and the β-glucuronidase (GUS) coding sequence were fused in pCAMBIA2...

文献信息：Kacar, Yildiz等.  发表于【Plant and Animal Genome】 | 请登录

简介：Citrus is an important commodity worldwide and produced in tropical and subtropical regions around the world. Genetic transformation of Citrus via Agrobacterium is a valuable technique for citrus improvement due to difficulties of conventional citrus breeding. In the present study we carried out genetic transformation studies through Agrobacterium rhizogenes ATCC15834 including RolABCD genes to in...

文献信息：K Kazmi, Syeda Khan, Saifullah A Mirbahar, Ameer A Kayani, Hammad等.  发表于【The Natural Products Journal】 | 请登录

简介：Kinnow mandarin (Citrus reticulata blanco) is the popular fruit crop in the world. In this study 12.5 % sodium dodecyl sulphate polyacryl amide gel electrophoresis (SDS-PAGE) method was used to observe comparative protein production pattern in different tissue culture stages of Kinnow mandarin. In this study embryogenic calli, non embryogenic calli, nucellar embryo, in vitro grown plant and seeds ...

文献信息：Husain, Indriati Utami, Fitria Nanda Purwito, Agus Husni, Ali Mutaqin, Kikin H Susanto, Slamet等.  2015-11-03 发表于【Horticultural Biotechnology Research/http://sciencefl ora.org/journals/index.php/hbr/】 |  , 请登录

简介：The aim of this research is a study about direct somatic embryogenesis from mature seed and plantlet regeneration of Citrus nobilis variety SoE. Plant material for somatic embryogenesis is the mature seed of Tangerine SoE. 6-benzylaminopurine (BAP) 3 mg/L supplemented to Murashige and Skoog (MS) modified medium was induced direct somatic embryos (SE) 4 weeks after treatments. MS modified medium wi...

文献信息：Endo, T. Fujii, H. Sugiyama, A. Nakano, M. Nakajima, N. Ikoma, Y. Omura, M. Shimada, T等.  2015-11-30 发表于【Plant science : an international journal of experimental plant biology】 |  , 请登录

简介：To explore the transcription factors associated with carotenoid metabolism in citrus fruit, one transcription factor (CubHLH1) was selected through microarray screening in Satsuma mandarin (Citrus unshiu Marc.) fruit, which was treated with exogenous ethylene or gibberellin (GA), accelerating or retarding carotenoid accumulation in peel, respectively. The amino acid sequence of CubHLH1 has homolog...

文献信息：Chin, Elizabeth Mishchuk, Darya LeVesque, Cynthia Foster, Elizabeth Godfrey, Kristine Slupsky, Carolyn等.  发表于【The FASEB Journal】 | 请登录

简介：Candidatus Liberibacter asiaticus (CLas), a non-culturable, phloem-limited bacterium, is one of the presumed causative agents of the devastating citrus disease known as Huanglongbing (HLB). HLB was first discovered in Florida in 2005, and has since spread throughout the state. It is now threatening both the California and Texas citrus industries. Diseased trees suffer from discolored leaves and fr...

文献信息：Cardoso, JC Abdelgalel, AM Chiancone, B Latado, RR Lain, O Testolin, R Germanà, MA等.  发表于【Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology】 | 请登录

简介：In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can pr...

文献信息：Amin, Hossein Shekafandeh, Akhtar等.  发表于【Jordan Journal of Agricultural Sciences】 |  , 请登录

简介：Callus production, somatic embryogenesis and plant regeneration were achieved from juice vesicles of Mexican lime. First, three types of culture media, Murashige and Skoog (MS), Murashige and Tucker (MT) and Grosser and Gmitter (H+H) and different levels of 2,4-Dicholorophenoxy acetic acid (2,4-D) were used to study their effects on production and proliferation of embryogenic calli from juice vesi...