文献信息：郭雁君,吉前华,周希琴,蒋惠,郭丽英,胡亚平等.  发表于【安徽农业科学】 |  , 请登录

简介：[目的]建立枳壳离体培养再生体系。[方法]采用均匀设计研究了不同时期枳壳茎尖、茎段、芽的愈伤组织诱导、增殖、分化、及生根情况。[结果]愈伤组织诱导率及生长量由高到低为春梢>夏梢>秋梢>冬梢;不同时期外植体诱导愈伤组织所需生长调节物质种类、浓度、配比不同,春梢茎段外植体诱导愈伤组织以NAA 0.2 mg/L+2,4-D 0.5 mg/L+KT 0.2 mg/L+IBA 0.1 mg/L为最好,夏梢茎段外植体诱导愈伤组织以NAA 0.2 mg/L+2,4-D 4.5 mg/L+KT 0.2 mg/L+IB...

文献信息：段艳欣,郭文武等.  发表于【青岛农业大学学报(自然科学版)】 |  , 请登录

简介：噻苯隆(Thidiazuron,TDZ)是一类人工合成的苯基脲衍生物,广泛用于多种植物的组织培养。本文以椪柑胚性愈伤组织和球形胚状体为试材,研究了不同浓度TDZ对柑橘胚性愈伤组织增殖与胚状体发育的影响;在此基础上,研究了TDZ对12种柑橘胚性愈伤组织转化的影响。结果表明,培养基中添加较低浓度的TDZ可促进椪柑胚性愈伤组织的增殖,以0.4mg/L为最佳,超过该浓度则起抑制作用。单独使用TDZ不能促进该品种体胚的发生与再分化,但可促进胚状体体积的...

文献信息：Darren CJ Wong, Crystal Sweetman and Christopher M Ford等.  2014-07-15 发表于【BMC Plant Biology,http://www.biomedcentral.com/1471-2229/14/186】 |  , 请登录

简介：Background: The genus Citrus encompasses major cultivated plants such as sweet orange, mandarin, lemon and grapefruit, among the world\'s most economically important fruit crops. With increasing volumes of transcriptomics data available for these species, Gene Co-expression Network (GCN) analysis is a viable option for predicting gene function at a genome-wide scale. GCN analysis is based on a \"g...

文献信息：Pascale Rossignol , Vladimir Orbovic , Vivian F. Irish 等.  2014-06-09 发表于【Scientia Horticulturae】 |  , 请登录

简介：Inducible gene expression systems, in which the level or timing of activity of a gene of interest can be controlled exogenously, are an effective means to assess gene function. However, such systems have not been widely employed in crop plants. Here we show that the glucocorticoid receptor (GR)-based inducible gene expression system functions in Citrange (Citrus sinensis x C. trifoliata) plants. W...

文献信息：Hongge Jia, Nian Wang*等.  2014-04-07 发表于【Plos One】 |  , 请登录

简介：Genetic modification, including plant breeding, has been widely used to improve crop yield and quality, as well as to increase disease resistance. Targeted genome engineering is expected to contribute significantly to future varietal improvement, and genome editing technologies using zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly int...

文献信息：Mohamed, El-Sawy A.Gomaa, Amina,Danial, Nancy等.  发表于【Plant Tissue Culture & Biotechnology】 |  , 请登录

简介：Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT-PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV-free. RAPD analysis was used to asses the genetic ...

文献信息：Thaísa Tessutti Pinheiro, Antonio Figueira and Rodrigo Rocha Latado等.  2014-08-10 发表于【Pinheiro et al. BMC Research Notes】 |  , 请登录

简介：Background: There had been many reports on genetic transformation of Citrus for functional genomic studies but
<br />few included genes associated with flower or fruit traits. A major reason for this might derive from the extensive
<br />juvenile stage of Citrus plants when regenerated from juvenile explants (epicotyls, cotyledon or calli), which delays
<br />the observation of the resulting pheno...

文献信息：Xiuping Zou &#8226; Erling Song &#8226; Aihong Peng &#8226;Yongrui He &#8226; Lanzhen Xu &#8226; Tiangang Lei &#8226;Lixiao Ya等.  2014-01-07 发表于【Plant Cell Tiss Organ Cult】 | 请登录

简介：To improve the resistance of citrus to canker
<br />disease caused by Xanthomonas axonopodis pv. citri (Xac),
<br />it is important to identify gene promoters that are specifically induced by pathogen infection. Here, we evaluated
<br />the functionality of PPP1 and hsr203J (Nicotiana tabacum
<br />L.) and gst1 (potato) pathogen-inducible promoters to drive
<br />expression of the b-glucuronidase ...

文献信息：Divanilde Guerra, Maria Teresa Schifino Wittmann, Sergio Francisco Schwarz ,Paulo Vitor Dutra de Souza , Mateus Pereira Gonzatto ,Roberto Luis Weiler 等.  发表于【Bragantia】 |  , 请登录

简介：Tetraploid citrus rootstocks may present different morphological characteristics and growth when compared to diploid ones.
<br />This worked aimed at comparing morphological characteristics and height growth of diploid and tetraploid plants from the
<br />rootstocks ‘Swingle’ citrumelo [C. paradise Macf. x Poncirus trifoliate (L.) Raf], citrange ‘Troyer’ (C. sinensis (L.) Osb. x P. trifoliata)...

文献信息：Shi-Xiao Xu &#8226; Dong-Fang Cai &#8226; Feng-Quan Tan &#8226;Yan-Ni Fang &#8226; Kai-Dong Xie &#8226;, Jude W. Grosser &#822等.  2014-07-01 发表于【Plant Cell Tissue and Organ Culture】 | 请登录

简介：Citrus somatic hybrids produced in the past years provide a novel opportunity to study the immediate effects of allopolyploidization on genome structure and methylation. Here, we present a first attempt to investigate the alterations in genome structure and methylation in three sets of citrus somatic allotetraploids and their diploid parents using amplified fragment length polymorphism (AFLP) and ...

文献信息：Hazarika, T. K.,Hazarika, B. N.,Shukla, A. C.等.  2014-10-31 发表于【Genetic Resources and Crop Evolution】 |  , 请登录

简介：Application of molecular markers, have now been increasingly adopted to address the problems in Citrus taxonomy. Compared to morphological data, molecular tools provide abundant information, highly efficient and are insensitive to environmental factors. Molecular markers has provided an ideal means for identifying genotypes, estimation of relatedness between different accessions and following inhe...

文献信息：Muniz Fabiana R,Muniz, Fabiana R.,Harakava, Ricardo,Alves Mourao Filho, Francisco de Assis,Stach-Machado, Dagmar R.,Rezende, Jorge A. M.,Febres, Vicen等.  2014-01-09 发表于【European Journal of Plant Pathology】 |  , 请登录

简介：Transgenic Citrus sinensis 'Hamlin' and 'Valencia' plants containing Citrus tristeza virus (CTV)-derived sequences were propagated and inoculated with CTV. For propagation, selected buds from transgenic and non-transgenic control plants were grafted onto C. aurantium and C. limonia rootstock plants. CTV inoculation was performed via viruliferous aphids (Toxoptera citricida), and viral detection po...

文献信息：Yahya Abdul-Halim，Chong Gun-Hean，Tan Chin-Ping等.  发表于【Scienceasia】 |  , 请登录

简介：The Merdeka lime is a new citrus hybrid with strong resistance against pests and disease. This work aims to provide information about the total phenolic content (TPC), total flavonoid (TF) content and antioxidant potential of the leaves, peel and pulp of this new hybrid along with those of its parent plants (Citrus hystrix and C. microcarpa). The TPC and TF contents were determined based on a colo...

文献信息：Mahadani, Pradosh,Ghosh, Sankar Kumar等.  2014-07-22 发表于【Molecular Biology Reports】 |  , 请登录

简介：The Consortium of Barcode of Life plant working group proposed to use the defined portion of plastid genes rbcL and matK either singly or in combination as the standard DNA barcode for plants. But DNA barcode based identification of biologically complex plant groups are always a challenging task due to the occurrence of natural hybridization. Here, we examined the use of indels polymorphism in trn...

文献信息：Jamilah Syafawati, Yaacob, Noraini, Mahmad,Rosna Mat, Taha,Normadiha, Mohamed,Anis Idayu, M. Y.,Azani, Saleh等.  发表于【The Scientific World Journal】 |  , 请登录

简介：Various explants (stem, leaf, and root) of Citrus assamensis were cultured on MS media supplemented with various combinations and concentrations (0.5-2.0 mgL -1) of NAA and BAP. Optimum shoot and root regeneration were obtained from stem cultures supplemented with 1.5 mgL -1 NAA and 2.0 mgL -1 BAP, respectively. Explant type affects the success of tissue culture of this species, whereby stem expla...

文献信息：Ramdan, R. Handaji, N. Beyahia, H. Ibriz, M.等.  发表于【Journal of Applied Biosciences】 |  , 请登录

简介：Objective: The aim of this work is to develop a protocol for callus induction of Moroccan Citrus rootstocks. Methodology and results: Callus cultures were initiated from embryos explants of five Citrus rootstocks ( Cleopatra mandarin, Rangpur lime, Citrus volkameriana, Trifoliate orange, Citrus aurantium) on Murashige and Tucker (MT) and Tucker (MT) basal media. Different concentrations of growth ...

文献信息：Jia Hongge,Wang Nian等.  2014-08-22 发表于【Plant Cell Reports】 |  , 请登录

简介：Key message Xanthomonas citri subsp. citri pretreatment before agroinfiltration could significantly promote transient expression in citrus leaves which were previously recalcitrant to agroinfiltration. Transient expression via agroinfiltration is widely used in biotechnology but remains problematic in many economically important plants. Xanthomonas citri subsp. citri (Xcc)-facilitated agroinfiltra...

文献信息：Cardoso, Jean C.Cardoso, Jean C.Germana, Maria Antonieta,Latado, Rodrigo R.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：Citrus, and particularly sweet oranges, are very recalcitrant to anther culture. In this paper it was evaluated for the first time the response of 27 genotypes of Citrus sinensis and of one hybrid C. clementina x C. sinensis, to in vitro anther culture. Ten genotypes of sweet oranges showed embryogenic callus induction, mostly blood sweet oranges genotypes, such as Tarocco, Moro and Sanguinelli. I...

文献信息：Balwinder, Singh等.  发表于【British Biotechnology Journal】 |  , 请登录

简介：The goal of this study was to evaluate the effect of desiccation and chilling treatments on somatic embryogenesis of rough lemon ( Citrus jambhiri Lush.). Styles were cultured on seven culture media (MS I-MS VII) containing Benzylaminopurine (BAP), Kinetin (KN) and Malt Extract for cell proliferation and somatic embryo development. Cell proliferation was maximum on MS IV media but maximum cultures...

文献信息：Zou, X. P.，Song, E. L，Peng, A. H.，He, Y. R.，Xu, L. Z.，Lei, T. G.，Yao, L. X.，Chen, S. C.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：To improve the resistance of citrus to canker disease caused by Xanthomonas axonopodis pv. citri (Xac), it is important to identify gene promoters that are specifically induced by pathogen infection. Here, we evaluated the functionality of PPP1 and hsr203J (Nicotiana tabacum L.) and gst1 (potato) pathogen-inducible promoters to drive expression of the beta-glucuronidase (GUS) reporter gene in tran...