文献信息：Divanilde Guerra, Maria Teresa Schifino Wittmann, Sergio Francisco Schwarz ,Paulo Vitor Dutra de Souza , Mateus Pereira Gonzatto ,Roberto Luis Weiler 等.  发表于【Bragantia】 |  , 请登录

简介：Tetraploid citrus rootstocks may present different morphological characteristics and growth when compared to diploid ones.
<br />This worked aimed at comparing morphological characteristics and height growth of diploid and tetraploid plants from the
<br />rootstocks ‘Swingle’ citrumelo [C. paradise Macf. x Poncirus trifoliate (L.) Raf], citrange ‘Troyer’ (C. sinensis (L.) Osb. x P. trifoliata)...

文献信息：Shi-Xiao Xu &#8226; Dong-Fang Cai &#8226; Feng-Quan Tan &#8226;Yan-Ni Fang &#8226; Kai-Dong Xie &#8226;, Jude W. Grosser &#822等.  2014-07-01 发表于【Plant Cell Tissue and Organ Culture】 | 请登录

简介：Citrus somatic hybrids produced in the past years provide a novel opportunity to study the immediate effects of allopolyploidization on genome structure and methylation. Here, we present a first attempt to investigate the alterations in genome structure and methylation in three sets of citrus somatic allotetraploids and their diploid parents using amplified fragment length polymorphism (AFLP) and ...

文献信息：Hazarika, T. K.,Hazarika, B. N.,Shukla, A. C.等.  2014-10-31 发表于【Genetic Resources and Crop Evolution】 |  , 请登录

简介：Application of molecular markers, have now been increasingly adopted to address the problems in Citrus taxonomy. Compared to morphological data, molecular tools provide abundant information, highly efficient and are insensitive to environmental factors. Molecular markers has provided an ideal means for identifying genotypes, estimation of relatedness between different accessions and following inhe...

文献信息：Muniz Fabiana R,Muniz, Fabiana R.,Harakava, Ricardo,Alves Mourao Filho, Francisco de Assis,Stach-Machado, Dagmar R.,Rezende, Jorge A. M.,Febres, Vicen等.  2014-01-09 发表于【European Journal of Plant Pathology】 |  , 请登录

简介：Transgenic Citrus sinensis 'Hamlin' and 'Valencia' plants containing Citrus tristeza virus (CTV)-derived sequences were propagated and inoculated with CTV. For propagation, selected buds from transgenic and non-transgenic control plants were grafted onto C. aurantium and C. limonia rootstock plants. CTV inoculation was performed via viruliferous aphids (Toxoptera citricida), and viral detection po...

文献信息：Yahya Abdul-Halim，Chong Gun-Hean，Tan Chin-Ping等.  发表于【Scienceasia】 |  , 请登录

简介：The Merdeka lime is a new citrus hybrid with strong resistance against pests and disease. This work aims to provide information about the total phenolic content (TPC), total flavonoid (TF) content and antioxidant potential of the leaves, peel and pulp of this new hybrid along with those of its parent plants (Citrus hystrix and C. microcarpa). The TPC and TF contents were determined based on a colo...

文献信息：Mahadani, Pradosh,Ghosh, Sankar Kumar等.  2014-07-22 发表于【Molecular Biology Reports】 |  , 请登录

简介：The Consortium of Barcode of Life plant working group proposed to use the defined portion of plastid genes rbcL and matK either singly or in combination as the standard DNA barcode for plants. But DNA barcode based identification of biologically complex plant groups are always a challenging task due to the occurrence of natural hybridization. Here, we examined the use of indels polymorphism in trn...

文献信息：Jamilah Syafawati, Yaacob, Noraini, Mahmad,Rosna Mat, Taha,Normadiha, Mohamed,Anis Idayu, M. Y.,Azani, Saleh等.  发表于【The Scientific World Journal】 |  , 请登录

简介：Various explants (stem, leaf, and root) of Citrus assamensis were cultured on MS media supplemented with various combinations and concentrations (0.5-2.0 mgL -1) of NAA and BAP. Optimum shoot and root regeneration were obtained from stem cultures supplemented with 1.5 mgL -1 NAA and 2.0 mgL -1 BAP, respectively. Explant type affects the success of tissue culture of this species, whereby stem expla...

文献信息：Ramdan, R. Handaji, N. Beyahia, H. Ibriz, M.等.  发表于【Journal of Applied Biosciences】 |  , 请登录

简介：Objective: The aim of this work is to develop a protocol for callus induction of Moroccan Citrus rootstocks. Methodology and results: Callus cultures were initiated from embryos explants of five Citrus rootstocks ( Cleopatra mandarin, Rangpur lime, Citrus volkameriana, Trifoliate orange, Citrus aurantium) on Murashige and Tucker (MT) and Tucker (MT) basal media. Different concentrations of growth ...

文献信息：Jia Hongge,Wang Nian等.  2014-08-22 发表于【Plant Cell Reports】 |  , 请登录

简介：Key message Xanthomonas citri subsp. citri pretreatment before agroinfiltration could significantly promote transient expression in citrus leaves which were previously recalcitrant to agroinfiltration. Transient expression via agroinfiltration is widely used in biotechnology but remains problematic in many economically important plants. Xanthomonas citri subsp. citri (Xcc)-facilitated agroinfiltra...

文献信息：Cardoso, Jean C.Cardoso, Jean C.Germana, Maria Antonieta,Latado, Rodrigo R.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：Citrus, and particularly sweet oranges, are very recalcitrant to anther culture. In this paper it was evaluated for the first time the response of 27 genotypes of Citrus sinensis and of one hybrid C. clementina x C. sinensis, to in vitro anther culture. Ten genotypes of sweet oranges showed embryogenic callus induction, mostly blood sweet oranges genotypes, such as Tarocco, Moro and Sanguinelli. I...

文献信息：Balwinder, Singh等.  发表于【British Biotechnology Journal】 |  , 请登录

简介：The goal of this study was to evaluate the effect of desiccation and chilling treatments on somatic embryogenesis of rough lemon ( Citrus jambhiri Lush.). Styles were cultured on seven culture media (MS I-MS VII) containing Benzylaminopurine (BAP), Kinetin (KN) and Malt Extract for cell proliferation and somatic embryo development. Cell proliferation was maximum on MS IV media but maximum cultures...

文献信息：Zou, X. P.，Song, E. L，Peng, A. H.，He, Y. R.，Xu, L. Z.，Lei, T. G.，Yao, L. X.，Chen, S. C.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：To improve the resistance of citrus to canker disease caused by Xanthomonas axonopodis pv. citri (Xac), it is important to identify gene promoters that are specifically induced by pathogen infection. Here, we evaluated the functionality of PPP1 and hsr203J (Nicotiana tabacum L.) and gst1 (potato) pathogen-inducible promoters to drive expression of the beta-glucuronidase (GUS) reporter gene in tran...

文献信息：Xiao, S. X.Biswas, M. K.Li, M. Y.Deng, X. X.Xu, Q.Guo, W. W.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：Seedlessness, an important economic trait for fresh fruit, is among the prior goal for all citrus breeding programs. Symmetric somatic hybridization provides a new strategy for citrus seedless breeding by creating cybrids transferring mitochondrial DNA (mtDNA) controlled cytoplasmic male sterility (CMS) from the callus parent Satsuma mandarin (C. unshiu Marc.) to seedy cultivars. In this study, pr...

文献信息：解凯东,王晓培,王惠芹,梁武军,谢宗周,郭大勇,伊华林,邓秀新,Grosser Jude W.,郭文武等.  发表于【园艺学报】 |  , 请登录

简介：2009—2012年,以柑橘8个多胚性二倍体品种为母本与近年始花的5个异源四倍体体细胞杂种和1个同源四倍体分别杂交,培育三倍体新种质。连续4年共配置15个杂交组合,授粉4 442朵花,坐果1 484个,平均坐果率33.4%;用于幼胚挽救的果实1 075个,共培养幼嫩种子12 578颗,经生芽、生根诱导获得再生植株2 832个;以流式细胞仪对所有再生植株倍性检测,15个组合共获得三倍体401个,四倍体121个;移栽成活三倍体349个,四倍体98个。对W. 默科特 ×NH组合8...

文献信息：邹修平,彭爱红,刘琦琦,何永睿,王军政,许兰珍,雷天刚,姚利晓,陈善春等.  发表于【园艺学报】 |  , 请登录

简介：为了利用CecropinB(CB)抗菌肽基因提高柑橘对溃疡病的抗性,人工合成了两个含有信号肽的Cecropin B抗菌肽基因PR1aCB和AATCB。洋葱表皮瞬时表达分析表明,与非分泌型CB抗菌肽相比,PR1aCB和AATCB抗菌肽在细胞间隙中优势积累。进一步构建了CaMV 35S调控 PR1aCB和AATCB基因的植物表达载体,转化塔罗科血橙(Citrus sinensis Osbeck)上胚轴,获得转基因植株。GUS组织化学染色、PCR和Southern blot分析表明外源基因已成功整合入柑橘基因组。Rea...

文献信息：马岩岩,陈娇,吴天利,张军,钟广炎等.  发表于【园艺学报】 |  , 请登录

简介：以奥林达夏橙[Citrus sinensis(L.)‘Olinda’]为材料,克隆了CsTBL1基因编码起始位点上游长度为2 361 bp的启动子序列。生物信息学预测表明,该启动子中含有多个与逆境应答相关的顺式作用元件。为进一步分析CsTBL1启动子的功能,构建了该基因启动子与GUS基因融合的植物表达载体,并用浸花法转化拟南芥,对T3代转基因拟南芥进行GUS活性染色。结果显示,CsTBL1启动子在1～3 d龄幼苗所有组织中的活性都非常强,在7～20 d龄幼苗的子叶和根中的活...

文献信息：马园园,邹修平,彭爱红,许兰珍,何永睿,陈善春等.  发表于【果树学报】 |  , 请登录

简介：【目的】分析柠檬苦素类似物糖基转移酶基因(citLGT)在柑橘中的异位表达。【方法】构建了CaMV 35S启动子控制下citLGT的超量和RNAi抑制表达载体。采用农杆菌介导的遗传转化方法将上述植物表达载体分别转化‘锦橙’[Citrus sinensis(Linn.)Osbeck‘Jincheng’]上胚轴。经GUS组织化学染色及PCR检测共获得20株转基因阳性植株。利用qRT-PCR分析转基因植株中citLGT基因的表达。【结果】超量表达citLGT的转基因植株中citLGT的表达量显著高于...

文献信息：许兰珍,彭爱红,何永睿,姚利晓,雷天刚,刘小丰,姜国金,邹修平,陈善春等.  发表于【园艺学报】 |  , 请登录

简介：为了筛选在柑橘韧皮部中特异表达的启动子,构建GRP、Rolc、RSSl异源韧皮部特异启动子控制GUS报告基因的植物表达载体,转化枳[Poncirus trifoliata(L.)Raf.]上胚轴。GUS组织化学染色分析表明,3个启动子均显示了维管束组织特异性。其中,GRP启动子活性强,且具有严格的韧皮部组织特异性;Rolc启动子活性最强,在根和茎中呈组成型表达;RSSl启动子活性最弱。Real-time RT-PCR和GUS酶活性分析进一步证明了GRP启动子具有较强的韧皮部组织特异性...

文献信息：刘琦琦,邹修平,彭爱红,许兰珍,何永睿,陈善春等.  发表于【中国南方果树】 |  , 请登录

简介：为评价转抗菌肽基因Cecropin B的锦橙植株对柑桔溃疡病的抗性,分别用喷雾法和针刺法进行了离体叶接种柑桔溃疡病菌试验,并用Real-time PCR和Southern blot进一步分析了Cecropin B在转基因植株中的表达、整合情况。结果显示,与喷雾法相比,针刺接种处理的离体叶片显症时间短,发病率高,便于统计分析。针刺接种6天,大部分转基因植株发病率与非转基因植株无显著差异;其中,PR8、PR11、AAT8和AAT14单株的发病率分别为67.0%、62.9%、68.1%和5...

文献信息：H Wang，C Petri，L Burgos，N Alburquerque等.  发表于【Plant Cell Tissue & Organ Culture】 | 请登录

简介：A mannose selection system was adapted for Agrobacterium -mediated transformation of plum ( Prunus domestica L.) hypocotyl explants and the recovery of transgenic plants. Adventitious regeneration from non-transformed hypocotyl sections was inhibited when 3&#160;mg/l mannose, combined with 10&#160;mg/l sucrose, was added to the medium. Mature seed hypocotyl slices from the cultivar ‘Claudia Verde...