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  • 461. Factors affecting Agrobacterium-mediated transformation efficiency of kumquat seedling internodal stem segments

    文献信息:Yang, L., Hu, W., Xie, Y. M., Li, Y., Deng, Z. N.等.  发表于【Scientia Horticulturae】 |  , 请登录

    简介:Kumquat (Fortunella crassifolia Swingle.) has a short juvenile phase of 2-3 years and it can flower and bear fruit several times per year. It is, therefore, helpful for citrus to evaluate functional genes rapidly. In the present experimentation, a comprehensive and systematic study was performed to improve the transformation efficiency of kumquat, which was usually less than 4.1% in previous repor...

    462. Biolistic transformation of Carrizo citrange (Citrus sinensis Osb. x Poncirus trifoliata L. Raf.)

    文献信息:Wu, H., Acanda, Y., Jia, H. G., Wang, N. A., Zale, J.等.  发表于【Plant Cell Reports】 | 请登录

    简介:The development of transgenic citrus plants by the biolistic method. A protocol for the biolistic transformation of epicotyl explants and transgenic shoot regeneration of immature citrange rootstock, cv. Carrizo (Citrus sinensis Osb. x Poncirus trifoliata L. Raf.) and plant regeneration is described. Immature epicotyl explants were bombarded with a vector containing the nptII selectable marker and...

    463. Induction of parthenogenetic haploid plants using gamma irradiated pollens in \'Hirado Buntan\' pummelo (Citrus grandis L. Osbeck)

    文献信息:Wang, S. M., Lan, H., Jia, H. H., Xie, K. D., Wu, X. M., Chen, C. L., Guo, W. W.等.  发表于【Scientia Horticulturae】 |  , 请登录

    简介:Homozygous genotypes have great potential in breeding and genomic researches in higher plants. Induction of parthenogenesis via pollination with gamma-irradiated pollens is an efficient method to obtain haploid plants that can overcome the biological limitations of fruit trees. In this study, we investigated the induction of haploid plants in ‘Hirado Buntan’ pummlo (Citrus grandis) through in-si...

    464. Species discrimination of the genus Citrus and the hybridization analysis

    文献信息:Sun, Y. L., Jin, C. W., Hong, S. K.等.  发表于【Research Journal of Biotechnology】 | 请登录

    简介:The genus Citrus (Aurantioideae, Rutaceae) is the most important fruit crops all around the world. As many cultivated and weedy species/varieties exist in this genus, botanists hold different opinions on the taxonomy and phylogeny of the genus Citrus all the time. To evaluate the taxonomy and phylogenetic relationship of Citrus species, we collected 22 Citrus species and evaluated the genetic dive...

    465. A survey of FLS2 genes from multiple citrus species identifies candidates for enhancing disease resistance to Xanthomonas citri ssp. citri

    文献信息:Shi, Q. C., Febres, V. J., Jones, J. B., Moore, G. A.等.  发表于【Horticulture Research】 |  , 请登录

    简介:Pathogen-associated molecular patterns (PAMPs)-triggered immunity (PTI) is an important component of plant innate immunity. In a previous study, we showed that the PAMP flg22 from Xanthomonas citri ssp. citri (Xflg22), the causal agent of citrus canker, induced PTI in citrus, which correlated with the observed levels of canker resistance. Here, we identified and sequenced two bacterial flagellin/f...

    466. Effects of salinity on diploid (2x) and doubled diploid (4x) Citrus macrophylla genotypes

    文献信息:Ruiz, M., Quinones, A., Martinez-Alcantara, B., Aleza, P., Morillon, R., Navarro, L., Primo-Millo, E., Martinez-Cuenca, M. R.等.  发表于【Scientia Horticulturae】 |  , 请登录

    简介:Tetraploid (4x) citrus seedlings may be more tolerant to salt stress than diploid (2x) genotypes. Genome duplication in citrus changes both plant physiology and anatomy, leading plants to acquire differentiated capacities to uptake and transport mineral elements. To provide insight into this behaviour, 2x and 4x Citrus macrophylla (CM), seedlings were grown at moderate (40 mM NaCl) and high salini...

    467. Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing

    文献信息:Reyes, C. A., De Francesco, A., Ocolotobiche, E. E., Costa, N., Garcia, M. L.等.  发表于【Physiological and Molecular Plant Pathology】 |  , 请登录

    简介:Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection w...

    468. Citrus breeding, genetics and genomics in Japan

    文献信息:Omura, M., Shimada, T.等.  发表于【Breeding Science】 |  , 请登录

    简介:Citrus is one of the most cultivated fruits in the world, and satsuma mandarin (Citrus unshiu Marc.) is a major cultivated citrus in Japan. Many excellent cultivars derived from satsuma mandarin have been released through the improvement of mandarins using a conventional breeding method. The citrus breeding program is a lengthy process owing to the long juvenility, and it is predicted that marker-...

    469. Production of Three New Grapefruit Like Cybrids Confirmed by Plant Mitochondrial Intron, Chloroplast and Nuclear DNA Markers to Improve Citrus Canker Resistance

    文献信息:Omar, A. A., Murata, M., Yu, Q. B., Gmitter, F. G., Chase, C. D., Graham, J. H., Grosser, J. W.等.  发表于【In Vitro Cellular & Developmental Biology-Animal】 | 请登录

    简介:The production of cybrids, combining nucleus of a species with alien cytoplasmic organelles, is a valuable method used for improvement of various crops. Moreover, this technology considers a non-GMO biotechnology strategy. In citrus, Cybrid plants can be produced as a by-product of somatic fusion. Several citrus cybrids have been created by somatic hybridization. Through the cell fusion between nu...

    470. Screening of \'King\' mandarin (Citrus nobilis Lour) x Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis

    文献信息:Martinez-Cuenca, M. R., Quinones, A., Forner-Giner, M. A.等.  发表于【Scientia Horticulturae】 |  , 请登录

    简介:Iron (Fe) chlorosis is one of the commonest problems in calcareous soils and plant tolerance is deter-
    <br />mined mainly by rootstocks. This work evaluates new citrus hybrids’ tolerance to Fe chlorosis compared with the most widely used rootstocks in Spain: Citrus macrophylla (CM, Fe chlorosis-tolerant) and Carrizo citrange (CC, Fe chlorosis-sensitive). Growth parameters, Fe concentration, photo...

    471. Induction of somatic embryogenesis in woody plants

    文献信息:Isah, T.等.  发表于【Acta Physiologiae Plantarum】 | 请登录

    简介:Somatic embryogenesis, the in vitro developmental program by which somatic cells are reprogrammed to undergo cellular and molecular changes that make them competent to produce somatic embryos, has been achieved with many woody plants. The program involves the stages of competence acquisition, induction and expression of the morphogenic pathway by the cultured cells and tissues. The ability to expr...

    472. Single-pollen genotyping to estimate mode of unreduced pollen formation in Citrus tamurana cv. Nishiuchi Konatsu

    文献信息:Honsho, C., Sakata, A., Tanaka, H., Ishimura, S., Tetsumura, T.等.  发表于【Plant Reproduction】 | 请登录

    简介:2n pollen formed by FDR in citrus. The Japanese local citrus cultivar, Nishiuchi Konatsu (Citrus tamurana hort. ex Tanaka; NK hereafter), has the ability to produce unreduced 2n pollen grains, allowing generation of polyploid progenies via sexual polyploidization. In this study, we developed a method of single-pollen genotyping for citrus and applied it to the analysis of transmission of heterozyg...

    473. A Database of Simple Sequence Repeat (SSR) Marker-Based DNA Profiles of Citrus and Related Cultivars and Germplasm

    文献信息:Hong, J. H., Chae, C. W., Choi, K. J., Kwon, Y. S.等.  发表于【Korean Journal of Horticultural Science & Technology】 | 请登录

    简介:The present study investigated identification of cultivars through phylogenetic analysis of 108 Citrus varieties and related cultivars using simple sequence repeat (SSR) markers. Two hundred three SSR primer pairs were used to detect polymorphic markers among 8 Citrus cultivars consisting of 4 mandarins, 1 orange, 1 tangor, 1 tangelo, and 1 pumelo. Eighteen SSR primer pairs were reproducible and s...

    474. Overexpression of a Modified Plant Thionin Enhances Disease Resistance to Citrus Canker and Huanglongbing (HLB)

    文献信息:Hao, G. X., Stover, E., Gupta, G.等.  发表于【Frontiers in Plant Science】 |  , 请登录

    简介:Huanglongbing (HLB or citrus greening disease) caused by Candidatus Liberibacter asiaticus (Las) is a great threat to the US citrus industry. There are no proven strategies to eliminate HLB disease and no cultivar has been identified with strong HLB resistance. Citrus canker is also an economically important disease associated with a bacterial pathogen (Xanthornonas citri). In this study, we chara...

    475. Tetraploidization in citrus rootstocks: effect of genetic constitution and environment in chromosome duplication

    文献信息:Guerra, D., Schifino-Wittmann, M. T., Schwarz, S. F., Weiler, R. L., Dahmer, N., de Souza, P. V. D.等.  发表于【Crop Breeding and Applied Biotechnology】 |  , 请登录

    简介:The objective of this study was to identify polyploid plants from citrus rootstocks and to evaluate the influence of the genetic constitution and of the environment on the frequency of chromosome duplication. Populations obtained from rootstock seeds of Trifoliata [Poncirus trifoliate (L.) Raf], citrumelo \'Swingle\' [C. paradise Macf. x P. trifoliate], citrange \'Troyer\' [C. sinensis (L.) Osb. x...

    476. Application of Somatic Embryogenesis in Woody Plants

    文献信息:Guan, Y., Li, S. G., Fan, X. F., Su, Z. H.等.  发表于【Frontiers in Plant Science】 |  , 请登录

    简介:Somatic embryogenesis is a developmental process where a plant somatic cell can dedifferentiate to a totipotent embryonic stem cell that has the ability to give rise to an embryo under appropriate conditions. This new embryo can further develop into a whole plant. In woody plants, somatic embryogenesis plays a critical role in clonal propagation and is a powerful tool for synthetic seed production...

    477. High-throughput sequencing and degradome analysis reveal altered expression of miRNAs and their targets in a male-sterile cybrid pummelo (Citrus grandis)

    文献信息:Fang, Y. N., Zheng, B. B., Wang, L., Yang, W., Wu, X. M., Xu, Q., Guo, W. W.等.  发表于【Bmc Genomics】 |  , 请登录

    简介:Background: G1 + HBP is a male sterile cybrid line with nuclear genome from Hirado Buntan pummelo (C. grandis Osbeck) (HBP) and mitochondrial genome from \"Guoqing No. 1\" (G1, Satsuma mandarin), which provides a good opportunity to study male sterility and nuclear-cytoplasmic cross talk in citrus. High-throughput sRNA and degradome sequencing were applied to identify miRNAs and their targets in G...

    478. Citrus Functional Genomics and Molecular Modeling in Relation to Citrus sinensis (Sweet Orange) Infection with Xylella fastidiosa (Citrus Variegated Chlorosis)

    文献信息:Dwivedi, U. N., Tiwari, S., Prasanna, P., Awasthi, M., Singh, S., Pandey, V. P.等.  发表于【Omics-a Journal of Integrative Biology】 | 请登录

    简介:Citrus are among the economically most important fruit tree crops in the world. Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa infection, is a serious disease limiting citrus production at a global scale. With availability of citrus genomic resources, it is now possible to compare citrus expressed sequence tag (EST) data sets and identify single-nucleotide polymorphisms (SNPs) wit...

    479. Identification and Evaluation of Root Specific Promoters for Efficient Gene Expression in Citrus

    文献信息:Dutt, M., Grosser, J. W.等.  发表于【In Vitro Cellular & Developmental Biology-Animal】 | 请登录

    480. Genetic diversity in lime (Citrus aurantifolia Swing.) and lemon (Citrus limon (L.) Burm.) based on quantitative traits in India

    文献信息:Dubey, A. K., Sharma, R. M. ,Awasthi, O. P., Srivastav, M., Sharma, N.等.  发表于【Agroforestry Systems】 | 请登录

    简介:The aim of this study was to collect and characterize lime and lemon accessions based on quantitative traits and to investigate genetic diversity in these species collected from the different agroclimatic zones of the India. Pomological diversity of 53 lime and 12 lemon accessions were analyzed based on 11 quantitative traits. The unweighted pair-group method with arithmetic mean analysis showed t...

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