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  • 521. 冷诱导基因转化柠檬的研究

    文献信息:姜航,胡威,屈汉金,周潇,张凤,邓子牛,龙桂友等.  发表于【湖南农业科学】 | 请登录

    522. Genetic transformation of Citrus sinensis \'Hamlin\' with attacin A driven by a phloem tissue-specific promoter for resistance to Candidatus Liberibacter spp

    文献信息:Tavano, E. C. da R.,Vieira, M. L. C.,Alves-Mourao Filho, F. de A.,Harakava, R.,Mendes, B. M. J.等.  请登录

    简介:Huanglongbing (HLB), associated to the bacteria Candidatus Liberibacter spp. which colonizes the phloem, is currently considered the most destructive citrus disease. Genetic transformation with genes that code for antibacterial peptides associated to phloem-specific promoters is considered an important strategy to control this disease. The objective of this work was to produce transgenic sweet ora...

    523. Efficient in vitro propagation and rooting of adult explants of citrus rootstocks

    文献信息:Tallon C. I.,Cordoba F.,Porras I.,Perez-Tornero O等.  请登录

    简介:The influence of the basal medium and different plant growth regulators on the efficient micropropagation of nodal explants from mature trees of sour orange and \'Cleopatra\' mandarin citrus rootstocks was studied. The preference of the two citrus rootstock shoot cultures for high-salt media, like MS or DKW, was observed. Several combinations of BA and AD, KIN or GA were made in an attempt to opti...

    524. Radiosensitivity of seeds and nodal segments of citrus rootstocks irradiated in vitro with gamma-rays from 137Cs

    文献信息:Tallon C. I.,Cordoba F.,Porras I.,Perez-Tornero O.等.  请登录

    简介:The first step in a breeding program involving physical mutagenesis by radiation is to evaluate the sensitivity in the plant material. Seeds of Citrus aurantium and C. macrophylla and nodal segments, where the buds were completely removed, from micropropagated explants of macrophylla, were used to determine their radiosensitivity to gamma radiation. A 137Cs source of gamma-rays was used to deliver...

    525. RNA interference against the three Citrus tristeza virus genes encoding silencing suppressors confers complete resistance to the virus in transgenic Mexican lime plants

    文献信息:Soler N.,Plomer M.,Fagoaga C.,Moreno P.,Navarro L.,Flores R.,Pena L.等.  发表于【<Go to ISI>://CABI:20153128821】 | 请登录

    简介:Citrus tristeza virus (CTV), the causal agent of the most devastating viral disease of citrus, has evolved three silencing suppressor proteins acting at intra- (p23 and p20) and inter-cellular level (p20 and p25) to overcome host antiviral defense. RNA interference (RNAi), an approach based on using dsRNA to trigger RNA silencing, has been widely used for generating transgenic plants resistant aga...

    526. Micro-shoot tip grafting as tool for production of disease free foundation block of Citrus reticulate Blanco \'Khasi\' mandarin

    文献信息:Rakes, Sanabam,Handique P. J.,Sunitibala Huidrom等.  发表于【<Go to ISI>://CABI:20153128813】 | 请登录

    简介:This study was carried out to produce viral disease free scion bank of \'Khasi\' mandarin ( Citrus reticulate Blanco) using four Citrus species namely \'Kachai\' lime ( Citrus jambhiri Lush), Champra maounthabi ( Citrus jambhiri Lush), Solom ( Citrus sps.) and Phouheiree ( Citrus sps) through micrografting techniques as viral disease affects the longevity of citrus orchards and quality of fruits. ...

    527. Chromosome instability in \'Carrizo\' citrange+ Citrus macrophylla somatic hybrids

    文献信息:Pensabene-Bellavia G.,Ruiz M.,Aleza P.,Olivares-Fuster O.,Ollitrault P.,Navarro L.等.  发表于【<Go to ISI>://CABI:20153128818】 | 请登录

    简介:Somatic hybridization by protoplast fusion is an effective technique for citrus rootstock breeding. It theoretically allows the accumulation of all genes of the parents irrespective of their heterozygosity level and therefore the addition of complementary dominant traits. In the present work, protoplasts were isolated from callus of C. macrophylla and from leaves of \'Carrizo\' citrange ( C. sinen...

    528. Somatic Hybridization and Cybridization Via Protoplast Fusion Technology for Citrus Improvement

    文献信息:Omar Ahmad A.,Murata Mayara,Altaf Sadaf,Chase Christine D.,Graham James H.,Grosser Jude W.等.  发表于【In Vitro Cellular & Developmental Biology-Plant】 | 请登录

    529. Development of citrus cultivar identification by CAPS markers and parentage analysis

    文献信息:Ninomiya T.,Shimada T.,Endo T.,Nonaka K.,Omura M.,Fujii H.等.  发表于【<Go to ISI>://CABI:20153165388】 | 请登录

    简介:The establishment of a method for citrus cultivar identification is required to protect domestic citrus producers and breeders\' rights. In order to establish a scientific method for citrus cultivar identification with marked reliability, it is necessary to develop appropriate DNA markers. Accordingly, this study aimed to establish such a method based on CAPS markers to realize expedient cultivar ...

    530. New low seeded mandarin ( Citrus reticulate) and lemon ( C. Limon) selections obtained by gamma irradiation

    文献信息:Montanola M. J.,Galaz A.,Gambardella M.,Martiz J.等.  发表于【<Go to ISI>://CABI:20153128801】 | 请登录

    简介:Gamma irradiation of buds is a technique that has been successfully used in citrus breeding programs that seek to produce seedless or low seeded selections of known cultivars. In 2007, the Pontificia Universidad Catolica de Chile CITRUS lab with FONDEF funds started a new breeding program of mandarins and lemons using this technique. The gamma irradiated and grafted buds were grown in commercial n...

    531. Applications of citrus shoot-tip grafting in vitro

    文献信息:Juarez J.,Aleza P.,Navarro L.等.  发表于【<Go to ISI>://CABI:20153128812】 | 请登录

    简介:The main application of shoot-tip grafting in vitro (STG) is to control graft-transmissible pathogens that requires the use of healthy trees in the new plantings. The routine application of STG using 0.1-0.2 mm shoot tips is very efficient for elimination of all citrus graft-transmissible pathogens from local or imported varieties. It has allowed the recovery of hundreds of healthy cultivars and t...

    532. Evaluation the effective factors on shoot proliferation of Washington navel orange in tissue culture medium

    文献信息:Jahromi A. A.,Dardan M.等.  发表于【Indian Journal of Fundamental and Applied Life Sciences】 | 请登录

    简介:The present study was conducted to evaluate the effective factors on prosperity percent of shoot proliferation of Washington navel orange in vitro culture by using young flashes having bud in completely randomized design. For this purpose were selected young shoots having bud with length of 1.5 cm as explants and following disinfection by solution of sodium hypo chloride 1% and washing by distille...

    533. Characterization of seed and embryo abortion during fruit development in citrus cultivars pollinated by \'Nishiuchi Konatsu\' ( Citrus tamurana) and a preliminary trial of embryo rescue in a

    文献信息:Honsho C.,Tsuruta K.,Ryuto K.,Sakata A.,Kuroki S.,Nishiwaki A.,Tetsumura T.等.  发表于【<Go to ISI>://CABI:20153128751】 | 请登录

    简介:\'Hyuganatsu\' (HY) is a self-incompatible citrus cultivar grown in specific prefectures in Japan. However, its bud mutation, \'Nishiuchi Konatsu\' (NK), is self-compatible, although the majority of seeds are aborted in mature fruits. In our previous studies, we revealed that NK produces a proportion of unreduced 2 n pollen grains, resulting in unusual seed development. In this study, we therefore...

    534. Evaluation of transgenic citrus for disease resistance to HLB and canker

    文献信息:Dutt M.,Barthe, G. A.,Orbovic V.,Irey M.,Grosser J. W等.  发表于【<Go to ISI>://CABI:20153128844】 | 请登录

    简介:Sweet orange and grapefruit cultivars were transformed with gene(s) encoding antimicrobial peptides or systemic acquired resistance (SAR) proteins. Each gene was under control of an enhanced CaMV 35S promoter. Several genes were also under control of a phloem specific Arabidopsis SUC2 ( AtSUC2) promoter. A number of clones of each transgenic line were evaluated for resistance to Huanglongbing (HLB...

    535. New cybrids resulting from asexual pathway: a promise of cybridization for creating new citrus rootstocks and cultivars

    文献信息:Dambier D.,Petit F.,Barantin P.,Ollitrault P.等.  发表于【<Go to ISI>://CABI:20153128794】 | 请登录

    简介:Cybridization should be a way to induce phenotypical variations in highly heterozygous secondary citrus species or genotypes, without breaking their very complex multilocus structure by sexual recombination. It should have particular interest to manipulate the nucleo-cytoplasmic male sterility, to transfer tolerance traits with cytoplasmic determinisms and eventually to improve tolerance to oxidat...

    536. Micropropagation for evaluation of new citrus somatic hybrid rootstocks

    文献信息:Bordas M.,Torrents J.,Navarro L.等.  发表于【<Go to ISI>://CABI:20153128772】 | 请登录

    简介:This research work presents the development of a protocol for the micropropagation of three allotetraploid somatic hybrid rootstocks, obtained at IVIA from two hybridizations: Citrus macrophylla+citrange Carrizo and C. macrophylla+ C. taiwanica. We have determined the conditions for the in vitro introduction and the establishment of micropropagation lines in the laboratory, with a high efficient m...

    537. Genetically Modified Citrus Rootstocks Expressing a Synthetic Cationic Antimicrobial Peptide Slows Down Bacterial Infection in the Non-transgenic Scion

    文献信息:Bergey Daniel,Dutt Manjul,Barthe Gary,Irey Mike,Grosser Jude等.  发表于【In Vitro Cellular & Developmental Biology-Plant, <Go to ISI>://WOS:000359929900035】 | 请登录

    简介:Synthetic cationic antimicrobial peptides with broad-spectrum antimicrobial activity have been developed that improve the molecular stability, antimicrobial activity and enhance disease resistance to several plant and animal pathogens. We expressed a gene encoding a synthetic chimera of the cecropin A and melittin antimicrobial peptides (AMP) into two citrus rootstocks. Twenty-two transgenic diplo...

    538. Effect of mutagens on regeneration and growth of in vitro grown epicotyl segments of rough lemon seedlings ( Citrus jambhiri Lush.)

    文献信息:Sukhjit, Kaur等.  2015-06-05 发表于【Journal of Applied and Natural Science】 | 请登录

    简介:The present study on the effect of mutagens on regeneration and growth of in vitro grown epicotyls seg-
    <br />ments of rough lemon seedlings (Citrus jambhiri Lush.) was carried out during the years 2009- 2010 and 2010- 2011
    <br />in the Tissue Culture Laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana. Develop-
    <br />mental characteristics of the in vitro grown epico...

    539. Symptoms induced by transgenic expression of p23 from Citrus tristeza virus in phloem-associated cells of Mexican lime mimic virus infection without the aberrations accompanying constitutive expressio

    文献信息:Soler Nuria,Fagoaga, Carmen,Lopez Carmelo,Moreno Pedro,Navarro Luis,Flores Ricardo,Pena Leandro等.  发表于【Molecular Plant Pathology】 | 请登录

    简介:Citrus tristeza virus (CTV) is phloem restricted in natural citrus hosts. The 23-kDa protein (p23) encoded by the virus is an RNA silencing suppressor and a pathogenicity determinant. The expression of p23, or its N-terminal 157-amino-acid fragment comprising the zinc finger and flanking basic motifs, driven by the constitutive 35S promoter of cauliflower mosaic virus, induces CTV-like symptoms an...

    540. Direct regeneration of shoot from axillary bud of Citrus reticulate

    文献信息:Shende, C. B ,Manik, S. R.等.  发表于【International Journal of Agricultural Technology,http://www.ijat-aatsea.com】 |  , 请登录

    简介:Young axillary buds of mature plant of Citrus reticulata were cultured on MS medium (1992) containing 6-Benzyl amino purine (BAP) 0.25, 0.50,1.00,1.50 & 2.00 mg/l. The best results for multiple shoots were obtained with 1.50 mg/l. The highest degree of Callus was induced on BAP 1.00 mg/l plus NAA 0.25 mg/l. The transfer of multiple shoots on rooting medium containing NAA 0.25 mg/l induced good roo...

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