文献信息：Xiao, S. X.Biswas, M. K.Li, M. Y.Deng, X. X.Xu, Q.Guo, W. W.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：Seedlessness, an important economic trait for fresh fruit, is among the prior goal for all citrus breeding programs. Symmetric somatic hybridization provides a new strategy for citrus seedless breeding by creating cybrids transferring mitochondrial DNA (mtDNA) controlled cytoplasmic male sterility (CMS) from the callus parent Satsuma mandarin (C. unshiu Marc.) to seedy cultivars. In this study, pr...

文献信息：解凯东,王晓培,王惠芹,梁武军,谢宗周,郭大勇,伊华林,邓秀新,Grosser Jude W.,郭文武等.  发表于【园艺学报】 |  , 请登录

简介：2009—2012年,以柑橘8个多胚性二倍体品种为母本与近年始花的5个异源四倍体体细胞杂种和1个同源四倍体分别杂交,培育三倍体新种质。连续4年共配置15个杂交组合,授粉4 442朵花,坐果1 484个,平均坐果率33.4%;用于幼胚挽救的果实1 075个,共培养幼嫩种子12 578颗,经生芽、生根诱导获得再生植株2 832个;以流式细胞仪对所有再生植株倍性检测,15个组合共获得三倍体401个,四倍体121个;移栽成活三倍体349个,四倍体98个。对W. 默科特 ×NH组合8...

文献信息：邹修平,彭爱红,刘琦琦,何永睿,王军政,许兰珍,雷天刚,姚利晓,陈善春等.  发表于【园艺学报】 |  , 请登录

简介：为了利用CecropinB(CB)抗菌肽基因提高柑橘对溃疡病的抗性,人工合成了两个含有信号肽的Cecropin B抗菌肽基因PR1aCB和AATCB。洋葱表皮瞬时表达分析表明,与非分泌型CB抗菌肽相比,PR1aCB和AATCB抗菌肽在细胞间隙中优势积累。进一步构建了CaMV 35S调控 PR1aCB和AATCB基因的植物表达载体,转化塔罗科血橙(Citrus sinensis Osbeck)上胚轴,获得转基因植株。GUS组织化学染色、PCR和Southern blot分析表明外源基因已成功整合入柑橘基因组。Rea...

文献信息：马岩岩,陈娇,吴天利,张军,钟广炎等.  发表于【园艺学报】 |  , 请登录

简介：以奥林达夏橙[Citrus sinensis(L.)‘Olinda’]为材料,克隆了CsTBL1基因编码起始位点上游长度为2 361 bp的启动子序列。生物信息学预测表明,该启动子中含有多个与逆境应答相关的顺式作用元件。为进一步分析CsTBL1启动子的功能,构建了该基因启动子与GUS基因融合的植物表达载体,并用浸花法转化拟南芥,对T3代转基因拟南芥进行GUS活性染色。结果显示,CsTBL1启动子在1～3 d龄幼苗所有组织中的活性都非常强,在7～20 d龄幼苗的子叶和根中的活...

文献信息：马园园,邹修平,彭爱红,许兰珍,何永睿,陈善春等.  发表于【果树学报】 |  , 请登录

简介：【目的】分析柠檬苦素类似物糖基转移酶基因(citLGT)在柑橘中的异位表达。【方法】构建了CaMV 35S启动子控制下citLGT的超量和RNAi抑制表达载体。采用农杆菌介导的遗传转化方法将上述植物表达载体分别转化‘锦橙’[Citrus sinensis(Linn.)Osbeck‘Jincheng’]上胚轴。经GUS组织化学染色及PCR检测共获得20株转基因阳性植株。利用qRT-PCR分析转基因植株中citLGT基因的表达。【结果】超量表达citLGT的转基因植株中citLGT的表达量显著高于...

文献信息：许兰珍,彭爱红,何永睿,姚利晓,雷天刚,刘小丰,姜国金,邹修平,陈善春等.  发表于【园艺学报】 |  , 请登录

简介：为了筛选在柑橘韧皮部中特异表达的启动子,构建GRP、Rolc、RSSl异源韧皮部特异启动子控制GUS报告基因的植物表达载体,转化枳[Poncirus trifoliata(L.)Raf.]上胚轴。GUS组织化学染色分析表明,3个启动子均显示了维管束组织特异性。其中,GRP启动子活性强,且具有严格的韧皮部组织特异性;Rolc启动子活性最强,在根和茎中呈组成型表达;RSSl启动子活性最弱。Real-time RT-PCR和GUS酶活性分析进一步证明了GRP启动子具有较强的韧皮部组织特异性...

文献信息：刘琦琦,邹修平,彭爱红,许兰珍,何永睿,陈善春等.  发表于【中国南方果树】 |  , 请登录

简介：为评价转抗菌肽基因Cecropin B的锦橙植株对柑桔溃疡病的抗性,分别用喷雾法和针刺法进行了离体叶接种柑桔溃疡病菌试验,并用Real-time PCR和Southern blot进一步分析了Cecropin B在转基因植株中的表达、整合情况。结果显示,与喷雾法相比,针刺接种处理的离体叶片显症时间短,发病率高,便于统计分析。针刺接种6天,大部分转基因植株发病率与非转基因植株无显著差异;其中,PR8、PR11、AAT8和AAT14单株的发病率分别为67.0%、62.9%、68.1%和5...

文献信息：H Wang，C Petri，L Burgos，N Alburquerque等.  发表于【Plant Cell Tissue & Organ Culture】 | 请登录

简介：A mannose selection system was adapted for Agrobacterium -mediated transformation of plum ( Prunus domestica L.) hypocotyl explants and the recovery of transgenic plants. Adventitious regeneration from non-transformed hypocotyl sections was inhibited when 3 mg/l mannose, combined with 10 mg/l sucrose, was added to the medium. Mature seed hypocotyl slices from the cultivar ‘Claudia Verde...

文献信息：de Oliveira, M. L.,de Lima Silva, C. C.,Abe, V. Y.,Costa, M. G.,Cernadas, R. A.,Benedetti, C. E.等.  发表于【Molecular plant-microbe interactions : MPMI】 |  , 请登录

简介：Mitogen-activated protein kinases (MAPK) play crucial roles in plant immunity. We previously identified a citrus MAPK (CsMAPK1) as a differentially expressed protein in response to infection by Xanthomonas aurantifolii, a bacterium that causes citrus canker in Mexican lime but a hypersensitive reaction in sweet oranges. Here, we confirm that, in sweet orange, CsMAPK1 is rapidly and preferentially ...

文献信息：Rongsheng, L. U.,Han, Meili,Lin, Rui,Yuefeng, M. A.,Yang, Yuxia,Jianlin, Qing等.  发表于【Guizhou Agricultural Sciences】 | 请登录

简介：By exploring factors which had influences during AFT gene transfer to citrus,transformation system of epicotyls from C.paradisi seedling epicotyls were established and transgenic plant were obtained in this paper.The transformation frequency was assessed in response to several different factors,including antibiotics type concentration,pre-culture time medium,co-cultivation media,dark incubation in...

文献信息：Wei, H. U.,Li, Yang,Xie, Yu Ming,Zhou, Xiao,Hong-Juan, G. E.,Da-Zhi, L. I.,Deng, Zi Niu等.  发表于【Journal of Hunan Agricultural University 】 |  , 请登录

简介：To increase the genetic transformation efficiency of sweet orange infected by Agrobacterium,an explant derived from internode stem segments of sucarri orange seedling was adopted to optimize light regimes and co-cultivation condition for the growth.Meantime,seedless gene was transferred to succari orange.The results revealed that explant from seedling had higher regeneration and transformation rat...

文献信息：Furman, Nicolás,Kobayashi, Ken,Zanek, Maria Cecilia,Calcagno, Javier,Garcia, Maria Laura,Mentaberry, Alejandro等.  发表于【Journal of Biotechnology】 | 请登录

简介：Citrus canker provoked by Xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. Xanthomonas infection is considered as an endemic disease in Northeast and Northwest Argentina, affecting as much as 10% of commercial citrus plantations. There is not known natural resistance neither in orange varieties nor in rootstocks used for ...

文献信息：Fu, X., and Liu, J.等.  发表于【BioMed Research International 】 |  , 请登录

简介：Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating diseases affecting the citrus industry worldwide. In our previous study, the canker-resistant transgenic sweet orange (Citrus sinensis Osbeck) plants were produced via constitutively overexpressing a spermidine synthase. To unravel the molecular mechanisms underlying Xcc resistance of the transgenic...

文献信息：de Carvalho, K.,de Campos, M. K.,Domingues, D. S.,Pereira, L. F.,Vieira, L. G.等.  发表于【Mol Biol Rep】 |  , 请登录

简介：Plant exposure to abiotic stresses leads to an accumulation of reactive oxygen species with the concomitant increase in antioxidant defense mechanisms. Previous studies showed that exogenous application of proline mitigate the deleterious effects caused by oxidative stress due to its ability to increase the activity of antioxidant enzymes. However, there are no reports of the effects of high endog...

文献信息：Xiuhua Chen , Jinyoung Y. Barnaby , Aswathy Sreedharan , Xiaoen Huang , Vladimir Orbovic , Jude W. Grosser , Nian Wang , Xinnian Dong , Wen-Yu等.  发表于【Physiological and Molecular Plant Pathology】 |  , 请登录

简介：Citrus canker is a devastating disease, caused by Xanthomonas axonopodis pv. citri (Xac). It is well
<br />established that the NPR1 gene plays a pivotal role in systemic acquired resistance (SAR) in Arabidopsis.In this study, we report the isolation and characterization of an NPR1 homolog from citrus, namely Citrus NPR1 homolog 1 (CtNH1). Sequence alignment and phylogenetic analysis indicate that...

文献信息： Sumontip Bunnag, Duangkamol Tangpong等.  发表于【AAB Bioflux 】 |  , 请登录

简介：This study was carried out to optimize the conditions for plantlet regeneration and genetic
<br />transformation of Citrus reticulata Blanco. with an antisense ACC oxidase gene mediated by
<br />Agrobacterium tumefaciens. The highest percentage of plantlet regeneration of 100 was successfully
<br />achieved on the MS medium supplemented with 3 μM BA, while the half-strength MS medium amended with...

文献信息：Chuanfu An 1 , Vladimir Orbovi&#263; 2 , Zhonglin Mou 1*等.  发表于【American Journal of Plant Sciences】 |  , 请登录

简介：Genetic transformation has become a promising tool for improvement of a variety of crop species. However, transferring genes across species, the presence of selectable marker genes, and bacteria-derived vector backbone sequences have raised considerable health and environmental concerns. Intragenic vector system-based intragenesis/cisgenesis is a new method using transgenic approach to achieving t...

文献信息：Alvarez, X.,Medina, C.,Cortés, R.,Aquea, F.,Rojas, S.,Arce-Johnson, P.等.  发表于【Acta Horticulturae】 | 请登录

简介：Citrus fruits are grown in tropical, sub-tropical and Mediterranean climates worldwide. Citrus varieties are currently grafted over selected rootstocks that influence their performance increasing fruit quality, disease resistance and tolerance to abiotic stresses. Although breeding programs have made important advances, increasing rootstock salinity tolerance has been a major problem for conventio...

文献信息：董美超,李进学,周东果,岳建强,高俊燕等.  发表于【中国果树】 |  , 请登录

简介：综述了柑橘引种、芽变选种、实生选种、杂交育种、组织培养育种、转基因育种和DNA分子标记辅助育种等研究成果;提出了柑橘品种选育中存在的问题,并对柑橘品种选育研究进行了展望。

文献信息：Cuenca, J.,Aleza, P.,Navarro, L.,Ollitrault, P.等.  发表于【Annals of Botany】 |  , 请登录

简介：Polyploidy is a major component of eukaryote evolution. Estimation of allele copy numbers for molecular markers has long been considered a challenge for polyploid species, while this process is essential for most genetic research. With the increasing availability and whole-genome coverage of single nucleotide polymorphism (SNP) markers, it is essential to implement a versatile SNP genotyping metho...