文献信息：张凌云,邓赞,安静等.   , 请登录

简介：富含脯氨酸蛋白4(PRP4,proline-rich protein 4)是一类响应胁迫并在细胞发育过程中起作用一种细胞壁蛋白。用RT-PCR克隆柑橘CsPRP4基因的正、反义cDNA序列,分别连接到T载体pMD–19T上;经限制性核酸内切酶2次双酶切,将CsPRP4基因的正、反义片段定向连接至双元质粒pFGC5941查耳酮合成酶A(CHSA)内含子的两端,构成反向重复序列;经菌液PCR和测序验证,成功构建了CsPRP4基因的RNA干涉载体;经农杆菌介导法转化柑橘,转基因植株具有明显不同于对...

文献信息：张文，胡威，张新宇，周敏，蒋巧巧，邓子牛，李大志等.   , 请登录

简介：【目的】为了获得沙田柚×枸橼杂交苗,【方法】以沙田柚为母本,枸橼为父本进行杂交育种,利用胚抢救技术获得杂种后代,并利用SRAP分子标记对亲本和子代的DNA进行扩增,分析亲本与杂交种之间扩增条带的差异。【结果】结果表明,最终获得27株杂交后代,引物组合Me6-Em5和Me10-Em12可鉴定其中5株为有性后代。【结论】MT+GA3(1 mg.L-1)培养基适合沙田柚×枸橼杂交胚抢救,利用SRAP标记可鉴定出杂交子代,可为以后利用BSA法构建抗感病池寻找柑橘...

文献信息：郑蓓蓓，谢宗周，郭文武等.   , 请登录

简介：采用高效液相色谱测定柑橘种间体细胞杂种[‘朋娜’脐橙(Citrussinensis Osbeck)+粗柠檬(C.jambhiri Lush)]和其两个融合亲本果实的类胡萝卜素含量,气相色谱测定可溶性糖及有机酸含量,并检测代谢过程中关键基因的表达,比较分析体细胞杂种果实品质遗传及基因表达特点。结果表明,体细胞杂种类胡萝卜素成分及含量均偏向粗柠檬亲本,柠檬酸、苹果酸积累量也偏向粗柠檬;而蔗糖含量处在中亲值;实时定量PCR技术检测类胡萝卜素代谢途径中的7个...

文献信息：SD Singer，KD Cox等.  发表于【Journal of Plant Biochemistry and Biotechnology】 | 请登录

简介：Membrane-localized H + -symporting sucrose transporters (SUC or SUT proteins) are involved in sucrose loading into the phloem of source tissues and sucrose uptake into sink tissues, which are essential events in the growth and development of higher plants. While many of these sucrose transporters are localized in the phloem, others function in sink tissues. In an attempt to gain insight into which...

文献信息：Zhijian T. Li , Frederick G. Gmitter Jr. ,Jude W. Grosser , Chunxian Chen , Dennis J. Gray等.  发表于【Tree Genetics & Genomes】 | 请登录

简介：MYB transcription factors play an important role in the activation and regulation of anthocyanin biosynthesis. Many such transcription factor genes from various plant species were well characterized at the molecular level. However, none were reported in Citrus . Here, we demonstrated the isolation, molecular characterization, and functional evaluation of a large number of Citrus genes belonging to...

文献信息：Eun-Ji Koh , Lijuan Zhou , Donna S. Williams , Jiyoung Park ,Ningyuan Ding , Yong-Ping Duan , Byung-Ho Kang等.  发表于【Protoplasma】 |  , 请登录

简介：Huanglongbing (HLB) is a destructive disease of citrus trees caused by phloem-limited bacteria, Candidatus Liberibacter spp. One of the early microscopic manifestations of HLB is excessive starch accumulation in leaf chloroplasts. We hypothesize that the causative bacteria in the phloem may intervene photoassimilate export, causing the starch to over-accumulate. We examined citrus leaf phloem cell...

文献信息：Soriano L, Mourao FDA, Camargo LEA, Cristofani-Yaly M, Latado RR, Pacheco CD, Azevedo FA, Mendes BMJ.等.  发表于【Plant Cell, Tissue and Organ Culture (PCTOC), 】 | 请登录

简介：Protoplast fusion between sweet orange and mandarin/mandarin hybrids scion cultivars was performed following the model "diploid embryogenic callus protoplast + diploid mesophyll-derived protoplast". Protoplasts were isolated from embryogenic calli of 'Pera' and 'Westin' sweet orange cultivars (Citrus sinensis) and from young leaves of 'Fremont', Nules', and 'Thomas' mandarins (C. reticulata), and ...

文献信息：Abbate L, Tusa N, Del Bosco SF, Strano T, Ruberto G, Renda A.等.  发表于【Food Research International】 |  , 请登录

简介：Three new somatic hybrids, namely an allotetraploid hybrid and two cybrids (2n and 4n), have been obtained by protoplast fusion of 'Valencia' sweet orange (Citrus sinensis L. Osbeck) + 'Femminello' lemon (C. limon L. Burm.). The chemical composition of the essential oils of the hybrids and their parents has been studied by gas chromatography (GC) combined with a flame ionisation detector (FID) and...

文献信息：Aleza P, Juarez J, Cuenca J, Ollitrault P, Navarro L.等.  发表于【Plant Cell Reports】 |  , 请登录

简介：The citrus fresh market demands the production of seedless citrus fruits, as seedy fruits are not accepted by consumers. The recovery of triploid plants has proven to be the most promising approach to achieve this goal, since triploids have very low fertility, are generally seedless and do not induce seeds in other cultivars by cross pollination. Triploid plants can be recovered by 2x x 4x sexual ...

文献信息：解凯东,王惠芹,王晓培,郭文武等.  发表于【2012年园艺植物染色体倍性操作与遗传改良学术研讨会】 |  , 请登录

简介：我国不仅是柑橘的起源地,而且柑橘种植面积和产量均居世界首位。但我国大约40%的柑橘有种籽,影响了其果实品质和商品价值。培育品质优良、食用方便、竞争力强的无籽品种一直是柑橘育种的重要目标。三倍体由于减数分裂不正常,配子败育,是天然的无籽类型。而培育三倍体的方法中,以四倍体与二倍体的倍性杂交最有效。本研究于2009-2011年连续3年,以HB柚、华农红柚、高斑柚、本地早橘、红橘、秋辉橘、克里曼丁橘、鄂柑1号椪柑、清见橘橙、...

文献信息：郭文武,解凯东,肖诗鑫,王淑明,王惠芹等.  发表于【2012年园艺植物染色体倍性操作与遗传改良学术研讨会】 |  , 请登录

简介：染色体倍性操作对克服柑橘珠心胚干扰等生殖障碍和促进柑橘无核育种、砧木改良等均具有十分重要的意义。一、体细胞杂交方面,10多年来,我们不断完善了柑橘原生质体操作技术;将转GFP标记材料用于体细胞杂交,揭示了体细胞杂种的再生优势现象;创制了40余例可用于接穗或砧木改良的种间、属间体细胞杂种;将优良体细胞杂种的花粉与我国特色地方良种杂交,结合幼胚挽救培养和流式细胞仪倍性鉴定,3年来创造了21个倍性杂交组合700余个株系的三倍...

文献信息：罗君琴,柯甫志,徐建国,王平,聂振朋,孙建华等.  发表于【浙江柑橘】 |  , 请登录

简介：柑橘胚性愈伤组织诱导技术在柑橘种质资源离体保存、理化诱变、原生质体融合、外源基因转化等柑橘种质保存及新种质创制过程中有着重要的基础研究地位。目前随着柑橘分子生物育种技术的快速发展,胚性愈伤组织作为柑橘理化诱变、原生质体融合、外源基因转化的理想起始材料,其在柑

文献信息：陈勇,雷天刚,何永睿,陈善春等.  发表于【安徽农业科学】 |  , 请登录

简介：从形态鉴别法、化学鉴别法、同工酶鉴别法、分子标记鉴别法几方面,综述了目前国内外关于柑橘材料鉴定方法的研究进展。

文献信息：陈勇等.  请登录

简介：杂交育种是目前国内外应用最普遍、最有效的一种育种方法。柑橘与其它果树不同,很多品种具有多胚性,而且如今栽培的很多优良品种都为无核品种,存在一定程度的性器官败育,这使得杂交育种方法在柑橘育种中的应用受到一定限制。采用胚挽救技术可以让杂种胚的发育不受珠心胚的干扰而获得再生植株,但在杂种胚挽救的同时也会不可避免地伴随珠心苗的产生。因此,建立一种高效的杂种胚挽救技术和杂种胚早期快速鉴定方法对减少珠心胚对杂交育种...

文献信息：杨海健等.   , 请登录

简介：柑橘作为世界产量第一的水果,在我国南方各省的农村经济构成中占有重要地位。它的发展,不仅影响着我国农民的收入水平,更对我国新农村建设发挥着重大作用。随着经济的发展,人民生活和消费水平的不断提高,消费者对果品市场中水果的品质和品种的多样化提出了更高的要求,这就使柑橘的品种更新显得尤为重要。本研究采用有性杂交育种的方法,通过配置不同杂交组合,创造了一批有性后代群体,为柑橘杂交育种准备了材料。采用不同花粉授粉马家柚...

文献信息：王淑明,曹洪波,王晓培,郭文武等.  发表于【2012年园艺植物染色体倍性操作与遗传改良学术研讨会】 |  , 请登录

简介：纯系材料在植物育种及基因组学研究中发挥着重要的作用,尤其在全基因组测序中有着明显的优越性,同时纯系材料还可用于物理图谱基因图谱分析、遗传转化等研究。柑橘类植物具有高度杂合、童期长、多胚性、树体高大等特性,很难通过常规育种获得纯系材料。花药培养是获得柑橘纯系材料快速、有效的途径。本研究以4种不同培养基,分别对红夏橙、早金甜橙、红橘、鄂柑1号椪柑、华农本地早橘、华农红抽、HB抽等7个品种的处于单核靠边期的花药进...

文献信息：曹洪波等.  请登录

简介：类胡萝卜素是植物生存必需的次生代谢类物质,参与众多的生物学过程,如光合作用、抗氧化保护、激素合成以及传粉等。类胡萝卜素也是人类健康所必需的生物活性成份,如提供维生素A前体,抗氧化和衰老,降低心血管疾病风险,以及参与人体免疫、胚胎发育和生殖等。植物是自然界中类胡萝卜素合成的主要生物体,也是人体中类胡萝卜素的最主要来源。因此,研究植物类胡萝卜素代谢调控机制是有效利用类胡萝卜素的重要基础。 柑橘中含有丰富多样的类...

文献信息：肖诗鑫,郭文武等.  发表于【2012年园艺植物染色体倍性操作与遗传改良学术研讨会】 |  , 请登录

简介：我国作为柑橘起源地,地方良种多但多数是有籽品种,影响了其果实品质和商品价值。因此,实现我国地方特色品种的无核化具有重要的理论和实际意义。温州蜜柑(Citrus unshiu Marc)为细胞质雄性不育(cytoplasmic male sterility,CMS)类型,本研究旨在针对性地开展温州蜜柑与其它有籽柑橘品种的对称融合研究,实现温州蜜柑细胞质雄性不育性状的有效转移。将国庆1号温州蜜柑愈伤组织悬浮系原生质体,分别与桃叶橙、早金甜橙等多籽甜橙的叶肉原生...

文献信息：Marutani-Hert M, Bowman KD, McCollum GT, Mirkov TE, Evens TJ, Niedz RP.等.  发表于【Plos One】 |  , 请登录

简介：Background: Citrus has an extended juvenile phase and trees can take 2-20 years to transition to the adult reproductive phase and produce fruit. For citrus variety development this substantially prolongs the time before adult traits, such as fruit yield and quality, can be evaluated. Methods to transform tissue from mature citrus trees would shorten the evaluation period via the direct production ...

文献信息：Sendin LN, Filippone MP, Orce IG, Rigano L, Enrique R, Pena L, Vojnov AA, Marano MR, Castagnaro AP.等.  发表于【Plant Pathology】 | 请登录

简介：susceptible host is diminished, indicating that the avrBs2 gene product could confer an adaptive advantage to the pathogen. The avrBs2 of Xanthomonas citri subsp. citri (Xcc), cause of citrus canker, shares 96% homology with avrBs2 of Xcv. To evaluate if Bs2 could recognize avrBs2 of Xcc in citrus plants and thereby activate plant defence mechanisms to increase resistance to canker, transient expr...