文献信息：Dubey, A. K., Sharma, R. M. ,Awasthi, O. P., Srivastav, M., Sharma, N.等.  发表于【Agroforestry Systems】 | 请登录

简介：The aim of this study was to collect and characterize lime and lemon accessions based on quantitative traits and to investigate genetic diversity in these species collected from the different agroclimatic zones of the India. Pomological diversity of 53 lime and 12 lemon accessions were analyzed based on 11 quantitative traits. The unweighted pair-group method with arithmetic mean analysis showed t...

文献信息：Aleza, P. ,Garcia-Lor, A. ,Juarez, J.,Navarro, L.等.  发表于【Plant Cell Tissue and Organ Culture】 |  , 请登录

简介：Somatic embryogenesis and plant regeneration are basic processes for the success of citrus somatic
<br />hybridization via protoplast fusion. In many cases, few embryos develop normally and only a small number of plants are recovered. The development of methodologies able to increase the recovery of plants after protoplast fusion experiments it is an important requirement to improve the efficiency...

文献信息：Agisimanto, D. ,Noor, N. M., Ibrahim, R., Mohamad, A.等.  发表于【Sains Malaysiana】 |  , 请登录

简介：Induced mutagenesis using gamma ray has been proven applicable to improve varieties of many genotypes of crop species. The effects of 60 Co gamma ray dosage on growth and callus induction of nucellus segments of Citrus reticulata cv. limau madu were investigated. The nucelli were exposed to gamma rays at doses of 10, 20, 40, 60, 80, 100 and 120Gy, followed by embryogenic callus ( EC ) induction o...

文献信息：Velazquez, K. Aguero, J. Vives, M. C. Aleza, P. Pina, J. A. Moreno, P. Navarro, L. Guerri, J.等.  2016-02-26 发表于【Plant Biotechnol J】 |  , 请登录

简介：The long juvenile period of citrus trees (often more than six years) has hindered genetic improvement by traditional breeding methods and genetic studies. In this work we have developed a biotechnology tool to promote transition from the vegetative to the reproductive phase in juvenile citrus plants by expression of the Arabidopsis thaliana or citrus FLOWERING LOCUS T (FT) genes using a Citrus lea...

文献信息：Hu, Wei Li, Wei Xie, Shenxi Fagundez, Sabrina McAvoy, Richard Deng, Ziniu Li, Yi等.  2016-01-23 发表于【Plant Cell, Tissue and Organ Culture (PCTOC)】 |  , 请登录

简介：The effects of a maize knotted1 (kn1) gene under the control of the cauliflower mosaic virus 35S promoter on genetic transformation efficiencies of six citrus genotypes were tested. The kn1 gene construct was used to transform ‘Pineapple’, ‘Hamlin’, ‘Sucarri’ and ‘Valencia’ sweet orange [Citrus sinensis (L.) Osbeck], ‘Carrizo’ Citrange [Citrus sinensis (L.) Osbeck × Poncirus trifoliate ...

文献信息：Hao, Guixia Pitino, Marco Duan, Yongping Stover, Ed等.  2016-01-05 发表于【Molecular Plant-Microbe Interactions】 | 请登录

简介：Overexpression of plant pattern-recognition receptors by genetic engineering provides a novel approach to enhance plant immunity and broad-spectrum disease resistance. Citrus canker disease associated with Xanthomonas citri is one of the most important diseases damaging citrus production worldwide. In this study, we cloned the FLS2 gene from Nicotiana benthamiana cDNA and inserted it into the bina...

文献信息：Chiancone, Benedetta Germanà, Maria Antonietta等.  发表于【In Vitro Embryogenesis in Higher Plants】 |  , 请登录

简介：Anther culture is a biotechnological method that allows to obtain, in one step, homozygous plants, very important to plant breeding, due to their numerous applications in mutation research, selection, genome sequencing, genetic analysis, and transformation. To induce the microspores, i.e., the immature male gametes, to switch from the normal gametophytic pathway to the sporophytic one, it is neces...

文献信息：Dutt, Manjul, Stanton, Daniel, Grosser, Jude W. and Fla State Hort, Soc等.  请登录

简介：Anthocyanins are beneficial bioflavonoids that have numerous roles in human health maintenance, disease prevention, and overall well-being. In addition, anthocyanins are key to the consumer appeal of many ornamental plants. Most citrus (Citrus L.) plants do not produce anthocyanins under warm tropical and subtropical conditions. Anthocyanin pigments, responsible for the "blood" color of blood oran...

文献信息：Orbovic, Vladimir, Calovic, Milica, Dutt, Manjul, Grosser, Jude W. and Barthe, Gary等.  请登录

简介：Transgenic plants of Citrus rootstock Carrizo citrange [Citrus sinensis (L) Osbeck x Poncirus trifoliata (L) Raf.] expressing a p35 gene from AcMNPV (Autographa californica moth Nuclear polyhedrosis virus) were produced. The p35 gene encodes a baculovirus protein which is suggested to be a negative regulator of apoptosis.Transgenic Carrizo plants carrying p35 gene in their genome had general pheno...

文献信息：Hongge Jia, Vladimir Orbovic, Jeffrey B. Jones, Nian Wang等.  发表于【Plant Biotechnology Journal】 | 请登录

简介：Citrus canker caused by Xanthomonas citri subspecies citri (Xcc) is a severe disease for most commercial citrus cultivars and responsible for significant economic losses worldwide. Generating canker‐\resistant citrus varieties will provide an efficient and sustainable solution to control citrus canker. Here, we report our progress in generating canker‐\resistant grapefruit by modifying the PthA4...

文献信息：Del Bosco, Sergio Fatta Napoli, Edoardo Mercati, Francesco Abbate, Loredana Carimi, Francesco Ruberto, Giuseppe等.  2015-12-10 发表于【Genetic Resources and Crop Evolution】 |  , 请登录

简介：Somatic cybridization is a technique based on inducted or spontaneous fusion between nuclear genome of one parent and either the cytoplasmic genome of the other parent or a combination of both donors. This approach is considered a powerful biotechnological tool in plant improvement since it enables to obtain novel intergenomic recombinations that are impossible to achieve by conventional crosses. ...

文献信息：何丹丹等.   , 请登录

简介：柑橘溃疡病(Citrus canker)是由地毯黄单胞杆菌柑橘致病变种(Xanthomonas nxonopodis pv. citri)引起的一种细菌性病害,目前尚无有效的方法可以根除柑橘溃疡病。利用病原物诱导启动子调控抗病基因的表达已成为植物抗病基因工程育种的主要策略之一。GST1病原诱导启动子来源于马铃薯,对病原菌具有广谱的响应。在转基因锦橙中,创伤和溃疡病菌侵染能高效诱导GST1控制的GUS报告基因的表达。因此通过解析GST1启动子顺式元件区域的功能,确定...

文献信息：张红红等.   , 请登录

文献信息：曾凡凡等.   , 请登录

简介：启动子是基因表达调控的重要元件,在植物转基因表达载体所选择的启动子中,应用最为广泛的是组成型启动子,如Ca MV 35S。然而组成型启动子会导致目的基因产物在整个植株大量积累,可能会影响植株的其他组织器官的正常生长发育,从而可能导致死亡。而组织特异性启动子(如根、茎、花、果特异表达启动子)可以将目的基因特异的表达于目标器官或组织,一方面避免基因的组成型表达对其他组织器官生长发育的影响,从而更好的进行基因功能验证,另一...

文献信息：曾凡凡等.   , 请登录

简介：启动子是基因表达调控的重要元件,在植物转基因表达载体所选择的启动子中,应用最为广泛的是组成型启动子,如Ca MV 35S。然而组成型启动子会导致目的基因产物在整个植株大量积累,可能会影响植株的其他组织器官的正常生长发育,从而可能导致死亡。而组织特异性启动子(如根、茎、花、果特异表达启动子)可以将目的基因特异的表达于目标器官或组织,一方面避免基因的组成型表达对其他组织器官生长发育的影响,从而更好的进行基因功能验证,另一...

文献信息：姜航 ，胡威， 屈汉金， 周潇， 张凤 ，邓子牛， 龙桂友等.  0015-05-21 发表于【湖南农业科学】 |  , 请登录

简介：将包含载体pPtcor∷8Ptcor8∷YFP的农杆菌EHA105转入到不抗寒的柠檬中,并对转化体系进行了优化.研究结果表明,再生培养基中的6-BA的浓度,卡那霉素(Kan)浓度以及除草剂(Basta)的浓度对柠檬的转化效率有明显影响;再生培养基配方为MT+ 0.5 mg/L 6-BA,添加75 mg/L kan或10 mg/L Basta时能够有效筛选到抗性芽,并获得了3株转基因柠檬植株,YFP阳性率为3.6%.

文献信息：Orbovic, V., Shankar, A., Peeples, M.E., Hubbard, C., and Zale, J.等.  发表于【Agrobacterium Protocols， Methods in Molecular Biology】 |  , 请登录

简介：Mature tissue protocol for production of transgenic Citrus plants via Agrobacterium-mediated transformation uses explants derived from branches of mature, fruit-bearing trees. Through the multiple cleaning steps consisting of grafting of apical tip meristems on rootstock plants grown under sanitary conditions, \"mother\" plants are produced that will serve as a source of budding material. These bu...

文献信息：Orbovic, V., Grosser, J. W.等.  发表于【Agrobacterium Protocols，Methods Mol Biol】 |  , 请登录

简介：The most frequently used method for production of citrus transgenic plants is via Agrobacterium -mediated transformation of tissues found on explants obtained from juvenile seedlings. Within the last decade and especially within the last 5–6 years, this robust method was employed to produce thousands of transgenic plants. With the newly applied screening methods that allow easier and faster detec...

文献信息：周潇 姜航 屈汉金 邓子牛 A·Gentile 龙桂友等.  发表于【果树学报】 |  , 请登录

简介：【目的】以YFP为报告基因,构建柑橘冷诱导基因及其启动子的植物表达载体。【方法】克隆获得枳冷诱导基因Ptcor8及其启动子p Ptcor8,柠檬中同源基因Clcor8及其启动子p Clcor8;双酶切含启动子的克隆载体和植物表达原始载体p1D4,连接获得含启动子的中间载体;再双酶切含冷诱导基因的克隆载体和中间载体,连接后获得重组质粒;通过冻融法将重组质粒导入农杆菌EHA105中。【结果】构建了p1D4/p Ptcor8-Ptcor8::YFP,p1D4/p Ptcor8-Clcor8::YFP和...

文献信息：张晓娜 刘继红等.  发表于【华中农业大学学报】 |  , 请登录

简介：基于生物信息学和实验预测,克隆了柑橘抗寒资源枳ptf-miR168a成熟序列和前体序列,并预测了其靶基因。结果表明:ptf-miR168a在植物中高度保守,靶基因是NAC(UCU2)和锌指结构基因(FEZ)家族同源基因;同时,构建了ptf-miR168a前体的超表达载体并在烟草中进行遗传转化,得到了18个转基因系,为进一步鉴定ptf-miR168a在低温胁迫应答中的调控作用奠定基础