文献信息：Boscariol-Camargo, Raquel L. Takita, Marco A. Machado, Marcos A.等.  2016-10-14 发表于【Tropical Plant Pathology】 |  , 请登录

简介：In sweet orange, varietal resistance is crucial to avoid citrus canker, a disease caused by Xanthomonas citri subsp. citri (Xcc) that is a significant problem for some of the world\'s leading producers. In the present study, transgenic sweet orange plants (Hamlin) overexpressing the A. thaliana Npr1 (non-expressor of pathogenesis-related [PR] genes) gene were produced and assessed for Xcc resistan...

文献信息：De Francesco, A. Costa, N. Garcia, M. L.等.  2016-10-28 发表于【Transgenic Research】 |  , 请登录

简介：Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1,...

文献信息：Widoretno, Wahyu Indriyani, Serafinah Martasari, Chairani Hakin, Rikza等.  2016-09-15 发表于【Agrivita】 |  , 请登录

简介：When obtaining plantlets, embryo maturation and simultaneous germination are important steps in plant micropropagation via somatic embryogenesis. Several studies have shown that the use of carbohydrates as a carbon source plays a significant role in inducing somatic embryo development in certain plant species. This study aimed to establish a somatic embryo maturation protocol for Batu 55 Mandarin ...

文献信息：Li, Fang Dai, Su-ming Deng, Zi-niu Li, Da-zhi Long, Gui-you Li, Na Li, Yi Gentile, Alexandra等.  2016-07-25 发表于【Journal of Integrative Agriculture】 |  , 请登录

简介：Agrobacterium-mediated transient expression assays are a convenient alternative to stable expression because they are simple, easy to perform, and achieve gene expression rapidly. This study investigated the factors affecting transient gene expression efficiency in citrus by observing the cryo-sectioning of leaf samples under a laser confocal microscope. These factors included the composition of t...

文献信息：Li, Fang Dai, Su-ming Deng, Zi-niu Li, Da-zhi Long, Gui-you Li, Na Li, Yi Gentile, Alexandra等.  2017-07-25 发表于【Journal of Integrative Agriculture】 |  , 请登录

简介：Agrobacterium-mediated transient expression assays are a convenient alternative to stable expression because they are simple, easy to perform, and achieve gene expression rapidly. This study investigated the factors affecting transient gene expression efficiency in citrus by observing the cryo-sectioning of leaf samples under a laser confocal microscope. These factors included the composition of t...

文献信息：Ben Mahmoud, Sulley Ramos, John E. Shatters, Robert G., Jr. Hall, David G. Lapointe, Stephen L. Niedz, Randall P. Rouge, Pierre Cave, Ronald D. Borovs等.  2016-07-26 发表于【Pesticide Biochemistry and Physiology】 |  , 请登录

简介：Diaprepes abbreviatus (L) is an important pest of citrus in the USA. Currently, no effective management strategies of D. abbreviatus exist in citriculture, and new methods of control are desperately sought. To protect citrus against D. abbreviatus a transgenic citrus rootstock expressing Bacillus thuringiensis Cyt2Cal, an insect toxin protein, was developed using Agrobacterium-mediated transformat...

文献信息：Cuenca, Jose Aleza, Pablo Garcia-Lor, Andres Ollitrault, Patrick Navarro, Luis等.  2016-12-23 发表于【Frontiers in Plant Science】 |  , 请登录

简介：Alternaria brown spot (ABS) is a serious disease affecting susceptible citrus genotypes, which is a strong concern regarding citrus breeding programs. Resistance is conferred by a recessive locus (ABSr) previously located by our group within a 3.3 Mb genome region near the centromere in chromosome III. This work addresses fine-linkage mapping of this region for identifying candidate resistance gen...

文献信息：Jia, Hongge Zhang, Yunzeng Orbovic, Vladimir Xu, Jin White, Frank F. Jones, Jeffrey B. Wang, Nian等.  2016-11-24 发表于【Plant Biotechnology Journal】 |  , 请登录

简介：Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease-resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyem...

文献信息：Kotoda, Nobuhiro Matsuo, Satoshi Honda, Ichiro Yano, Kanako Shimizu, Tokurou等.  2016-09-27 发表于【Horticulture Journal】 |  , 请登录

简介：Gibberellins (GAs) are involved in many aspects of plant growth and development in higher plants. In this study, we aimed to characterize Satsuma mandarin (Citrus unshiu Marc.) GA 2-oxidase genes encoding enzymes with GA inactivation activity because the accumulation of active GAs is regulated by the balance between their synthesis and inactivation. We showed that CuGA2ox4, CuGA2ox2/3, and CuGA2ox...

文献信息：Zhang, Yong-yan Zhang, Dong-min Zhong, Yun Chang, Xiao-jun Hu, Min-lun Cheng, Chun-zhen等.  2016-12-01 发表于【Scientia Horticulturae】 |  , 请登录

简介：A simple and efficient transformation method is important for plant genetic breeding and gene function analysis. In this study, we developed a in planta transformation approach for pommelo (Citrus maxima), which includes seed germination, seedling decapitation, Parafilm funnel-type-winding, Agrobacterium infection, co-culture, selective-culture, dark treatment for buds induction, transgene integra...

文献信息：Zou, Xiuping Jiang, Xueyou Xu, Lanzhen Lei, Tiangang Peng, Aihong He, Yongrui Yao, Lixiao Chen, Shanchun等.  2016-10-19 发表于【Plant Molecular Biology】 |  , 请登录

简介：Expression of synthesized cecropin B genes in the citrus phloem, where Candidatus Liberibacter asiaticus resides, significantly decreased host susceptibility to Huanglongbing. Huanglongbing (HLB), associated with Candidatus Liberibacter asiaticus bacteria, is the most destructive disease of citrus worldwide. All of the commercial sweet orange cultivars lack resistance to this disease. The cationic...

文献信息：李芳 ，邓子牛， 赵亚 ，李大志 ，戴素明等.  2016-08-19 发表于【中国农业科学】 |  , 请登录

简介：构建柑橘衰退病毒(Citrus tristeza virus,CTV)含p23的RNAi载体,以获得具有抗性的柑橘转基因植株。【方法】基于转化病毒基因介导抗性,根据NCBI公布的CTV基因组序列,查找p23保守序列,设计并克隆两条不同长度的片段。对两条片段和植物表达载体p BI 121进行双酶切和连接来构建RNAi载体。初步预测所构建的载体发生RNAi抗病毒的可行性。利用农杆菌介导的瞬时表达技术将含RNAi载体的农杆菌注射入CTV指示植物墨西哥莱蒙的叶片,利用GUS组织化...

文献信息：李芳 ，邓子牛， 赵亚 ，李大志， 戴素明等.  发表于【湖南农业大学学报(自然科学版)】 |  , 请登录

简介：基于转化病毒基因介导抗性,通过在植物表达载体p CAMBIA 2301上插入启动子–内含子–终止子的方式,构建一种含有发夹结构的、通用型强的RNAi载体骨架结构;以柑橘衰退病毒(citrus tristeza virus,CTV)的p25、p20和p23基因保守序列为模板设计正向片段和反向片段,先后与骨架载体连接,成功构建了3个RNAi载体(分别命名为ds2301–p25、ds2301–p20和ds2301–p23),并将载体ds2301–p23注射入墨西哥莱蒙叶片。制作p23基因的地高辛标记探针,用...

文献信息：何利刚， 蒋迎春 ，吴黎明 ，仝铸， 王志静 ，孙中海 ，许淼等.  发表于【湖南农业科学】 |  , 请登录

简介：桃叶橙[Citrus sinensis(L.)Osbeck cv.Taoyecheng]原产秭归县屈原镇龙马溪村,品质优良,被誉为\"峡橙三秀\"之一。然而,随着1990年脐橙产业发展壮大以来,桃叶橙种植面积逐渐萎缩,同时由于栽培品种混杂,品质性状表现参差不齐,阻碍了桃叶橙产业的稳定发展。为了复兴桃叶橙产业,推广地方特色品种,优化柑橘品种结构,项目组自2009年以来一直开展桃叶橙的提纯选优工作,通过长期调查搜集、品质测定和分子鉴定,最终筛选出果大、少核、高糖低酸...

文献信息：Sulley BenMahmoud a,John E. Ramos b, Robert G. Shatters Jr. b, David G. Hall b, Stephen L. Lapointe b, Randall P. Niedz b,PierreRougé c,Ronald D. Cave等.  2016-07-26 发表于【Pesticide Biochemistry and Physiology】 |  , 请登录

简介：Diaprepes abbreviatus (L.) is an important pest of citrus inthe USA. Currently, no effectivemanagement strategies ofD. abbreviatus exist incitriculture, and newmethods of control are desperately sought. Toprotect citrus against D. abbreviatus a transgenic citrus rootstock expressing Bacillus thuringiensis Cyt2Ca1, an insect toxin protein,was developed using Agrobacterium-mediated transformation of...

文献信息：伊华林， 李秋景， 程玉芳， 米兰芳， 汪爱云， 钟八莲， 陈健美， 苏华楠， 彭婷， 钟日芬， 林石鑫等.  发表于【中国南方果树】 |  , 请登录

简介： 近年来，我国柑桔育种取得了较大进展。对２００６ — ２０１４年我国审（认）定的柑桔新品种
<br />数量、类型，涉及的育种技术、人员和单位情况进行整理。结果显示，此期间，我国育种队伍不断壮
<br />大，核心力量逐渐形成，育种手段多样化程度有所提高；共审（认）定１１３个新品种，其中引种４１
<br />个，芽变选种（含珠心系变异） ５３个等，以异源四倍体体细胞杂种为父本再和二倍体杂交获得三倍
<br />体品种１个。同时，...

文献信息：许兰珍， 何永睿， 雷天刚， 彭爱红， 姚利晓， 姜国金， 李强， 邹修平， 陈善春等.  发表于【园艺学报】 |  , 请登录

简介：为分析转基因柑橘中外源基因整合拷贝数，基于实时荧光定量 PCR 法，建立通用、准确、
<br />简便的转基因柑橘外源基因拷贝数检测体系，利用该体系成功检测了 150 个转基因柑橘单株系外源基因
<br />整合拷贝数。结果表明，转基因柑橘中外源基因整合最低拷贝数为 1，最高达 5 个，其中 1、2 和 ≥ 3
<br />拷贝的单株数分别占总数的 40.0%、18.0%和 42.0%；采用 Southern blot 技术进行检测，结果基本一致，
<br />极少数单株经实时...

文献信息：吴黎明， 蒋迎春， 何利刚， 王志静， 仝铸， 孙中海， 许淼， 吴述勇， 廖胜才等.  发表于【园艺学报】 |  , 请登录

简介：‘金峡桃叶橙’是从‘桃叶橙 18 号’芽变中选育出的新品种。树体生长健壮，果实近圆球形，橙红色，果形指数 0.98，平均单果质量 140 g 以上。果肉橙黄色，少核或无核，高糖低酸，香气浓郁，质脆化渣，风味香甜。果汁含可溶性固形物 12.96%，可滴定酸 0.45%，维生素 C 304.5 mg · L -1 ，品质优。早结实，丰产稳产性好，发梢能力强，在湖北秭归地区 4 月中下旬开花，11 月下旬至 12 月上旬果实成熟。

文献信息：米兰芳， 汪爱云， 钟八莲， 陈健美， 苏华楠， 彭婷， 钟日芬， 林石鑫等.  发表于【果树学报】 |  , 请登录

简介：‘赣脐4号’是从‘纽荷尔’脐橙芽变中选育的脐橙新品种。该品种果实圆形或偏扁圆形，单果质量220~240 g，成熟果实外观漂亮，品质优，果肉脆嫩化渣，易剥皮，可溶性固形物含量12.1%，总酸含量0.47%，维生素C含量633.0mg·L -1 。树势中等，坐果率高，早结丰产性好。多点区域试验和品种比较试验表明，该品种成熟早，成熟期在9月下旬至10月上旬。

文献信息：邓崇岭， 陈传武， 唐艳， 伊华林， 邓光宙， 付慧敏， 刘冰浩， 罗鸿林， 范承彪等.  发表于【果树学报】 |  , 请登录

简介：‘桂橘一号’是从‘蜜广橘’芽变中选育的早熟柑橘新品种。果实扁圆形，果皮橙黄色，果面光滑，有光泽，果肉橙黄色，化渣，味清甜；平均单果质量46.77 g，最大单果质量63.3 g，果形指数0.72；每果实含种子0.85粒，单胚，子叶淡绿色。可食率78.01%，果汁率61.31%，可溶性固形物含量12.29％，全糖11.02%，可滴定酸0.64%，固酸比19.85，维生素C含量212.0 mg·g -1 ；品质上等。果实生育期160 d，在桂林兴安 10月上中旬成熟。桂北地区及...