文献信息：Wang Rong ,Fang Yan-Ni ,Wu Xiao-Meng, Qing Mei, Li Chao-Chao, Xie Kai-Dong ,Deng Xiuxin, Guo Wen-Wu等.  发表于【Plant Physiology】 | 请登录

简介：MicroRNA399 (miR399) regulates phosphate homeostasis in plants by down-regulating the expression of PHOSPHATE2 (PHO2, or UBC24 encoding the ubiquitin-conjugating E2 enzyme). We previously identified CsmiR399a.1 in a small RNA sequencing screen of a male-sterile somatic cytoplasmic hybrid (or cybrid) of pummelo (Citrus grandis). Here, we report that miR399 affects reproductive development and male ...

文献信息：Liu Xiaojuan, Zhao Chenning ,Gong Qin ,Wang Yue, Cao Jinping ,Li Xian, Grierson Donald ,Sun Chongde等.  发表于【Journal of experimental botany】 | 请登录

简介：Polymethoxylated flavones (PMFs), which accumulate exclusively in fruit peel of citrus, play important physiological and pharmacological roles but the genetic basis for the methylation of flavonoids has not been fully elucidated in citrus. Here we characterize a caffeoyl-CoA O-methyltransferase-like enzyme, designated CrOMT1. The expression pattern of CrOMT1 was highly correlated with the concentr...

文献信息：Yang Li, Liu Dechun, Hu Wei, Chun Yongqiang, Zhang Juanjuan, Liu Yong等.  2020-06-01 发表于【Scientia Horticulturae】 | 请登录

简介：The 'Majia' pomelo (Citrus maxima (Burm.) Merr., Rutaceae) is a distinctive local cultivar of Jiangxi province, China, that is famous for its good flavour and abundant lycopene content; however, each 'Majia' pomelo fruit usually contains approximately 120-200 large seeds. To obtain seedless 'Majia' pomelo fruit, we used pollen from sour pomelo (a local variety generally used for pollination) irrad...

文献信息：Zhang Min,Zhang Zequn,Wu Qun, Ke Fuzhi ,Xu Jianguo, Zhao Siqing, Wang Gang ,Zhang Chi等.  发表于【Horticultural Science】 | 请登录

简介：Chimeras occur spontaneously or artificially and are valuable in horticultural crop breeding. A new diploid citrus chimera, named `Hongrou Huyou' (abbreviated HH) (Citrus changshan-huyou + C. unshiu), was found during a bud sports investigation in China. The morphology, flesh carotenoid content, and molecular markers were evaluated in HH and the two grafted donors. The results showed that characte...

文献信息：Zhang Pan, Liu Xiaofeng, Yu Xin, Wang Fusheng, Long Junhong,Shen Wanxia,Jiang Dong,Zhao Xiaochun等.  2020-07-06 发表于【BMC Plant Biology】 | 请登录

简介：Background: Limonoids are major bioactive compounds that are produced by the triterpenoid metabolic pathway. The detailed biochemical process of limonoid biosynthesis and the mechanism of its molecular regulation remain elusive. The identification of transcription factors that regulate limonoid biosynthetic pathways is very important for understanding the underlying regulatory mechanisms. This inf...

文献信息：Yin Zhang,Junli Ye,Chaoyang Liu,Qiang Xu,Lichang Long,Xiuxin Deng等.  请登录

简介：Proanthocyanidins (PAs; or condensed tannins) are a major class of flavonoids that contribute to citrus fruit quality. However, the molecular mechanism responsible for PA biosynthesis and accumulation in citrus remains unclear. Here, we identify a PH4–Noemi regulatory complex that regulates proanthocyanidin biosynthesis in citrus. Overexpression of PH4 or Noemi in citrus calli activated the expre...

文献信息：Zhou Junhui, Li Dongdong, Wang Guoming, Wang Fuxi, Kunjal Merixia, Joldersma Dirk, Liu Zhongchi等.  发表于【Journal of Integrative Plant Biology】 | 请登录

简介：Fruit crops, including apple, orange, grape, banana, strawberry, watermelon, kiwifruit and tomato, not only provide essential nutrients for human life but also contribute to the major agricultural output and economic growth of many countries and regions in the world. Recent advancements in genome editing provides an unprecedented opportunity for the genetic improvement of these agronomically impor...

文献信息：谢幸男,杨莉,刘范,田娜,车婧如,靳三鹏,张永艳,程春振等.  发表于【园艺学报】 | 请登录

简介：以‘伏令夏橙’实生苗为材料进行原位转化体系建立及优化。首先,研究侵染部位对原位转化效率的影响:使用带有PBI121载体的农杆菌分别侵染5周龄‘伏令夏橙’实生苗上胚轴切口、顶芽切口(保留/去除真叶)2次,共培养3d后进行抗性筛选及暗处理,7周后提取叶片DNA用于转基因植株PCR鉴定;之后,以上胚轴切口作为侵染部位进一步研究苗龄对原位转化效率的影响。研究结果表明,5周龄上胚轴切口再生率为93.10%,转化率为20.68%;保留/去除真叶后侵染顶...

文献信息：孙立方,柯甫志,聂振朋,王平,孙建华,徐建国等.  发表于【浙江农业科学】 | 请登录

简介：柑橘病害严重影响其外观、品质和产量,给生产带来损失。本研究利用LP4/2A作为连接肽,构建AtNPR1和抗菌肽Cecropin B基因的共表达载体,为后期转化柑橘获得抗病材料做准备。以实验室前期构建的质粒为模板扩增AtNPR1基因,并人工合成连接肽LP4/2A和抗菌肽Cecropin B基因的编码序列。利用重叠PCR将AtNPR1和LP4/2A-Cecropin B重组,并连接至表达载体pBI121,获得以35S为启动子的35S:AtNPR1-LP4/2A-Cecropin B的共表达载体。同时,本研究还进一...

文献信息：戴文珊,王敏,刘继红等.  发表于【园艺学报】 | 请登录

简介：为了探明印度酸橘(Citrus reshni)脱落酸合成关键基因CrNCED1的功能,分析了CrNCED1的表达特性,并将其在柠檬中超表达获得转基因植株,对转基因植株进行脱水抗性分析。结果表明,CrNCED1在印度酸橘叶片中表达量最高;并且其表达受脱水、低温和ABA诱导,但不受盐诱导,其中ABA诱导上调作用强烈。超表达CrNCED1的转基因柠檬植株ABA含量显著高于野生型,脱水处理后叶片萎蔫程度比野生型轻,其相对失水率、MDA和活性氧含量均显著低于野生型,叶片气...

文献信息：Aljasim, Kawther, Dutt, Manjul, Grosser, Jude W. and Soares, Juliana M.等.  请登录

文献信息：Calovic, Milica, Chen, Chunxian, Yu, Qibin, Orbovic, Vladimir, Gmitter, Frederick G., Jr. and Grosser, Jude W.等.  请登录

简介：Six mandarin cultivars, Ponkan (Citrus reticulata), Willowleaf (Citrus deliciosa), Kinnow (Citrus nobilis x C. deliciosa), Murcott (purported C. reticulata x Citrus sinensis), W. Murcott [purported (C. reticulata x C. sinensis) x C. reticulata)], and Snack (purported C. reticulata hybrid), were used in protoplast fusion with different parental combinations to generate somatic hybrids. Sixty-five s...

文献信息：Qiang Li, Anhua Hu, Wanfu Dou, Jingjing Qi, Qin Long, Xiuping Zou, Tiangang Lei, Lixiao Yao, Yongrui He, Shanchun Chen等.  请登录

文献信息：Qiang Li, Ruirui Jia, Wanfu Dou, Jingjing Qi, Xiujuan Qin, Yongyao Fu, Yongrui He*, Shanchun Chen等.  请登录

文献信息：Sandhu, J. S., Nayyar, S., Kaur, A., Kaur, R., Kalia, A., Arora, A., Kaur, Y., Thind, S. K. and Chhabra, G.等.  2019-05-16 请登录

文献信息：Soares, Juliana M., Qiu, Wenming, Grosser, Jude W. and Dutt, Manjul等.  请登录

文献信息：Zou Xiuping, Long Junhong, Zhao Ke, Peng Aihong, Chen Min, Long Qin, He Yongrui, Chen Shanchun等.  2019-12-12 发表于【Plos One】 | 请登录

简介：The auxin early response gene Gretchen Hagen3 (GH3) plays dual roles in plant development and responses to biotic or abiotic stress. It functions in regulating hormone homeostasis through the conjugation of free auxin to amino acids. In citrus, GH3.1 and GH3.1L play important roles in responding to Xanthomonas citri subsp. citri (Xcc). Here, in Wanjingcheng orange (Citrus sinensis Osbeck), the ove...

文献信息：Shi Cai-Yun, Hussain Syed Bilal, Yang Huan, Bai Ying-Xin, Khan Muhammad Abbas ,Liu Yong-Zhong等.  发表于【Plant Science,<Go to ISI>://WOS:000494891900026】 | 请登录

简介：Citric acid homeostasis patterns and its content are diversified among the fruits of citrus cultivars, but the cause remained unclear. In this study we showed that changes of citric acid content were highly associated with the expression profiles of a P-type proton pump gene (CsPH8) in the fruits of six citrus cultivars; moreover, analysis of 21 different fruit samples indicated that the correlati...

文献信息：Liu Xiao, Guo Ling-Xia, Luo Li-Juan, Liu Yong-Zhong, Peng Shu-Ang等.  发表于【Plant Molecular Biology】 | 请登录

简介：Key message: At least eight MGT genes were identified in citrus and PtrMGT5 plays important role in maintaining Mg homeostasis in citrus by getting involved in the Mg absorption and transport. Abstract: Magnesium (Mg) is an essential macronutrient for plant growth and development, and the magnesium transporter (MGT) genes participate in mediate Mg2+ uptake, translocation and sequestration into cel...

文献信息：Geng Jingjing, Wei Tonglu, Wang Yue, Huang Xiaosan, Liu Ji-Hong等.  2020-07-19 发表于【Tree physiology】 | 请登录

简介：Basic helix-loop-helix (bHLH) family of transcription factors plays crucial role in regulating plant response to abiotic stress by targeting a large spectrum of stress-responsive genes. However, the physiological mechanisms underlying the transcription factors-mediated stress response are still poorly understood for most of the bHLH genes. In this study, transgenic pummelo (Citrus grandis) plants ...