文献信息：Jia Hongge, Orbovi Vladimir, Wang Nian等.  2019-03-25 发表于【Plant biotechnology journal】 | 请登录

简介： Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully in Duncan grapefruit via Xcc-facilitated agroinfiltration. Next, LbCas12a drive...

文献信息：Jain Mukesh, Munoz-Bodnar Alejandra, Gabriel Dean W.等.  发表于【Journal of Plant Physiology】 | 请登录

简介：The Lasbcp (CLIBASIA_RS00445) 1-Cys peroxiredoxin gene is conserved among all 13 sequenced strains of Candidatus Liberibacter asiaticus, the causal agent of Huanglongbing or "citrus greening" disease. LasBCP was previously characterized as a secreted peroxiredoxin with substrate specificity for organic peroxides, and as a potential pathogenicity effector. Agrobacterium-mediated transient expressio...

文献信息：Huang Ding, Yuan Yue, Tang Zhouzhou, Huang Yue, Kang Chunying, Deng Xiuxin, Xu Qiang等.  2019-07-15 发表于【 Plant, cell & environment】 | 请登录

简介：Blood orange is generally recognized to accumulate anthocyanins in its fruit pulp in a cold-inducible manner. We observed that the fruit peel of blood orange can also accumulate anthocyanins under ample light conditions. Interestingly, purple pummelo can accumulate anthocyanins only in its fruit peel but not in its pulp. The mechanism underlying the tissue specificity of anthocyanin accumulation i...

文献信息： He Yongrui, Jia Ruirui, Qi Jingjing, Chen Shanchun, Lei Tiangang, Xu Lanzhen, Peng Aihong, Yao Lixiao, Long Qin, Li Zhengguo, Li Qiang等.  2019-07-30 发表于【Gene】 | 请登录

简介：Genetic engineering approaches offer an alternative method to the citrus canker resistance breeding. The ethylene response factor (ERF) family is a member of families of transcription factors that are particular to plants and contribute significantly to biotic stress response and to plant growth. CsAP2-09 belongs to the citrus AP2/ERF transcription factor family. Initially, we proved the induction...

文献信息：邹修平, 范迪, 彭爱红, 何永睿, 许兰珍, 雷天刚, 姚利晓, 李强, 罗克明, 陈善春等.  请登录

简介：为了获得CsLOB1启动子较大片段删除的突变体,利用CRISPR/Cas9技术对CsLOB1启动子进行多位点编辑。通过在CsLOB1启动子的EBEPthA4区域及其上、下游设计不同的靶标位点,构建了2个植物表达载体pCas9CsLOB1:2sites和pCas9CsLOB1:3sites,分别对CsLOB1启动子同时进行2个位点和3个位点的编辑。测序结果表明pCas9CsLOB1:2sites和pCas9CsLOB1:3sites的基因编辑效率分别为64.7%和80.0%,突变体植株在2个sgRNA之间发生了DNA片段的删除。进一步的分...

文献信息：김호방等.  发表于【Applied Biological Chemistry】 | 请登录

简介：Many citrus cultivars have the polyembryony trait that develops many nucellar embryos alongside a single zygotic embryo in an individual seed by sporophytic apomixis. This unique botanical trait hinders citrus breeding by genetic hybridization and affects breeding efficiency and cost. Techniques to efficiently identify nucellar and zygotic individuals in citrus are still very limited. For a system...

文献信息：Geng Jingjing, Wei Tonglu, Wang Yue, Huang Xiaosan, Liu Ji-Hong等.  2019-07-19 发表于【Tree physiology】 | 请登录

简介： Basic helix-loop-helix (bHLH) family of transcription factors plays crucial role in regulating plant response to abiotic stress by targeting a large spectrum of stress-responsive genes. However, the physiological mechanisms underlying the transcription factors-mediated stress response are still poorly understood for most of the bHLH genes. In this study, transgenic pummelo (Citrus grandis) plants...

文献信息：Goulin Eduardo Henrique, Galdeano Diogo Manzano, Granato Lais Moreira, Matsumura Emilyn Emy, Dalio Ronaldo Jose Durigan, Machado Marcos Anton等.  2019-09-01 发表于【Microbiological research】 | 请登录

简介： Citrus crops have great economic importance worldwide. However, citrus production faces many diseases caused by different pathogens, such as bacteria, oomycetes, fungi and viruses. To overcome important plant diseases in general, new technologies have been developed and applied to crop protection, including RNA interference (RNAi) and clustered regularly interspaced short palindromic repeat (CRIS...

文献信息： de Lima Silva Caio Cesar, Shimo Hugo Massayoshi, de Felicio Rafael, Mercaldi Gustavo Fernando, Rocco Silvana Aparecida, Benedetti Celso Edua等.  2019-03-07 发表于【 Scientific Reports】 | 请登录

简介：Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that...

文献信息：da Rocha Tavano Eveline Carla, Erpen Ligia, Aluisi Bianca, Harakava Ricardo, Spotti Lopes Joao Roberto, Carneiro Vieira Maria Lucia, De Stefano Piedad等.  发表于【Journal of Horticultural Science & Biotechnology】 | 请登录

简介：An alternative approach to control Huanglongbing disease in Citrus is the development of transgenic plants expressing genes that may influence pathogen development. We report herein the production of sweet orange transgenic plants bearing gene constructs containing the antimicrobial gene attacin A (attA) under the control of phloem-specific promoters. Transcripts of the attA gene were accumulated ...

文献信息：Costa S N, Cortez P A, da Hora Almeida L A, Martins F M, Soares Filho W d S, Coelho Filho M A, Gesteira A d S等.  发表于【 Brazilian Journal of Botany】 | 请登录

简介：This work aimed to ascertain the triploid frequency of sexual hybridization and the pollen and ovary development in Citrus. The Fortune' mandarin (Citrus clementina Hort. ex Tan.xC. tangerina Hort. ex Tan.) accession was selected as female parent for this study based on its reproductive characteristics, mainly the monoembryony and self-incompatibility. The male parents used were the Cravo' (C. ret...

文献信息：Agisimanto Dita, Normah M. N., Ibrahim Rusli等.  发表于【Agrivita】 | 请登录

简介：Embryogenic cell (EC) growth and proliferation as well as somatic embryo induction were examined in a bioreactor culture using Murashige and Skoog basal medium particularly for the influence of 6-benzyladenine (BA) concentration, initial cell density and aeration rate. Embryogenic callus was induced from seeds of Citrus reticulata Blanco cv. Madu. The cell suspension in a 3-L bioreactor exhibited ...

文献信息： Acanda Yosvanis, Wang Chunxia, Levy Amit等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

简介：To understand how Citrus tristeza virus (CTV) replicates and moves inside the plant, it is critical to study the cellular interactions and localization of its encoded proteins. However, due to technical limitations, so far these studies have been limited to the nonnatural host Nicotiana benthamiana.Particle bombardment is a physical method to deliver nucleic acid and other biomolecules into the ce...

文献信息：Abbate L, Panno S, Mercati F, Davino S, Del Bosco S F等.  发表于【 European Journal of Plant Pathology】 | 请登录

简介：Four allotetraploid somatic hybrids of citrus, with potential for rootstock improvement, have been evaluated for their response to Citrus tristeza virus (CTV) infection. CTV is the most important viral pathogen affecting citrus production worldwide. Somatic combinations of Milam' lemon (Citrus jambhiri Lush.)+Sour orange (C. aurantium L Osb.), Calamondin (C. madurensis Lour.)+Keen' sour orange (C....

文献信息：Shimizu Tokurou等.  发表于【Jarq-Japan Agricultural Research Quarterly201】 | 请登录

简介：Current citrus breeding programs have three objectives: developing diverse varieties that satisfy assorted commercial needs; improving overall fruit quality; and releasing varieties quickly. However, these objectives may conflict with each other owing to the limited available orchard space, and conventional breeding systems have not progressed rapidly enough to resolve this problem. "Citrus Breedi...

文献信息：Sendin Lorena Noelia, Filippone Maria Paula等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

简介：Developing disease resistance is one of the most important components of any plant breeding program. Citrus traditional breeding methods (bud sport selection, crossbreeding, and other breeding channels) are a laborious task and often hampered by long juvenility, a high degree of heterozygosity, polyembryony, self-incompatibility, and abortion of reproductive organs. An interesting alternative to t...

文献信息：Peng A, Zou X, Xu L, He Y, Lei T, Yao L, Li Q, Chen S等.  发表于【In Vitro Cellular & Developmental Biology - Plant】 | 请登录

简介：The production of transgenic citrus plants from adult tissues is difficult because of low regeneration and transformation rates. To increase the transformation efficiency of adult citrus tissues, an improved protocol involving adult Citrus sinensis Osbeck ‘Tarocco’ blood orange tissues was developed. Explants were pre-incubated in a liquid medium prior to infection by Agrobacterium tumefaciens. ...

文献信息：Murata M M, Omar A A, Mou Z, Chase C D, Grosser J W, Graham J H等.  2019-01-07 发表于【Frontiers in Plant Science】 | 请登录

简介：Host disease resistance is the most desirable strategy for control of cirtus canker, a disease caused by a gram-negative bacterium Xanthomonas cirti subsp. citri. However, no resistant commerical citrus has been identified. Cybribization, a somatic hybribization approach that combines the organelle and nuclear genomes from different species, was used to create cybrids between citrus canker resista...

文献信息：Mei P, Song Z, Li Z a, Zhou C等.  请登录

简介：NADPH oxidases, encoded by rbohs (respiratory burst oxidase homologues), transfer electrons from NADPH to molecular oxygen (O-2) to generate superoxide anion (O-2(center dot-)), which is the first step in the formation of hydrogen peroxide (H2O2) in the plant-pathogen interaction system. In the present work, six citrus rbohs (Csrbohs) genes were identified in citrus, and their possible involvement...

文献信息：Lu Pengjun, Wang Shasha, Grierson Don, Xu Changjie等.  2019-01-01 发表于【Planta】 | 请登录

简介： Main conclusion Carotenoid accumulation and chromoplast development in orange were perturbed by carotenoid inhibitors, and candidate genes were identified via transcriptomic analysis. The role of CsPHT4;2 in enhancing carotenoid accumulation was revealed. Carotenoids are important plant pigments and their accumulation can be affected by biosynthesis inhibitors, but the genes involved were largely...