文献信息：王丽娟,龙俊宏,谢竹,吴柳,彭爱红,何永睿,龙琴,陈善春,邹修平等.  发表于【园艺学报】 | 请登录

简介：由柑橘黄单胞杆菌致病变种(Xanthomonas citri subsp. citri,Xcc)引起的柑橘溃疡病严重危害着柑橘产业的健康可持续发展。前期研究表明,CsWRKY22可能是溃疡病感病基因。从‘晚锦橙’(Citrus sinensis Osbeck)中克隆了CsWRKY22的两个等位启动子pCsWRKY22-1和pCsWRKY22-2,长度分别为1 428和1406bp。序列分析表明两个等位启动子序列一致性为91.76%。两个等位启动子含有多种参与植物防御反应和生长发育的顺式作用元件,相同元件的数量和位...

文献信息：胡安华,祁静静,张庆雯,陈善春,邹修平,许兰珍,彭爱红,雷天刚,姚利晓,龙琴,何永睿,李强等.  发表于【中国农业科学】 | 请登录

简介：【目的】克隆CsPGIP并分析其表达特性,转化柑橘得到超表达转基因株系,并进行柑橘溃疡病抗性评价,为柑橘溃疡病分子育种提供理论依据。【方法】从晚锦橙和四季橘中克隆柑橘CsPGIP,使用MEGA6进行多序列比对并构建系统发育树;采用在线软件BaCelLo和SignalP 4.0进行亚细胞定位和信号肽预测并用GFP瞬时表达确定CsPGIP在细胞内的定位;利用实时荧光定量PCR(qRT-PCR)比较接种溃疡病菌前后高感品种和高抗品种中柑橘CsPGIP的表达特性,分析溃疡病...

文献信息：葛廷,黄雪,谢让金等.  发表于【中国农业科学】 | 请登录

简介：【目的】多聚半乳糖醛酸酶是一类参与细胞壁降解的水解酶,在植物生长发育和器官脱落过程中发挥着重要作用。本研究克隆柑橘CitPG34及其启动子(CitPG34-P)并进行表达分析,为深入研究柑橘PG在幼果脱落过程的生物功能奠定基础。【方法】以‘塔罗科’血橙(Citrus sinensis L. Osbeck)为材料,克隆CitPG34及其启动子,利用ProtParam、Cello、CLUSTALX、MEGA5.2、PlantCARE等软件对其蛋白特性及启动子顺式作用元件进行分析预测;利用实时荧光定...

文献信息：Soler Nuria, Plomer Montserrat, Fagoaga Carmen, Moreno Pedro, Navarro Luis, Flore, Ricardo, Pena Leandro等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

简介：Conventional breeding of citrus types demands a long-term effort due to their complex reproductive biology and long juvenile period. As a compelling alternative, genetic engineering of mature tissues allows the insertion of specific traits into specific elite cultivars, including well-known and widely grown varieties and rootstocks, thus reducing the time and costs involved in improving and evalua...

文献信息：Singh Gurteg, Aulakh Pushpinder Singh, Sarao Navraj Kaur, Rattanpal Harinder Singh, Sidhu Gurupkar Singh等.  发表于【Agricultural Research】 | 请登录

简介：The molecular verification of different intra-specific mandarin hybrids was explored during 2014-2015. Simple sequence repeat (SSR) markers were employed to identify putative zygotic seedlings from hybrid progeny produced from different crosses in mandarin. The most informative SSR marker in the F-1 hybrids of "Daisy x Kinnow' cross was CCSM170 which identified 73% of zygotic seedlings followed by...

文献信息：Shimo Hugo Massayoshi, Terassi Carolina, Lima Silva Caio Cesar, de Lima Zanella Jackeline, Mercaldi Gustavo Fernando, Rocco Silvana Aparecida, Benedet等.  2019-03-21 发表于【Molecular plant pathology】 | 请登录

简介： Poly(A) tail shortening is a critical step in messenger RNA (mRNA) decay and control of gene expression. The carbon catabolite repressor 4 (CCR4)-associated factor 1 (CAF1) component of the CCR4-NOT deadenylase complex plays an essential role in mRNA deadenylation in most eukaryotes. However, while CAF1 has been extensively investigated in yeast and animals, its role in plants remains largely unk...

文献信息：Li Shao-Jia, Xie Xiu-Lan, Liu Sheng-Chao, Chen Kun-Song, Yin Xue-Ren等.  2019-11-30 发表于【 Food chemistry】 | 请登录

简介：Citrus fruit postharvest degreening is a critical stage in marketing, carried out by exposure to ethylene or ethephon. Genome-wide screening of the AP2/ERF superfamily indicated that a novel ERF-II (CitERF6) was shown to trans-activate the CitPPH promoter. Expression of CitERF6 is associated with both developmental and postharvest degreening in citrus fruit. Transient and stable over-expression of...

文献信息：Killiny Nabil, Nehela Yasser, Hijaz Faraj, Ben-Mahmoud Sulley K., Hajeri Subhas, Gowda Siddarame等.  发表于【Plant Biotechnology Reports】 | 请登录

简介：In this study, phytoene desaturase (PDS) in citrus and green fluorescent protein (GFP) in Nicotiana benthamiana (line 16c) were silenced by virus-induced gene silencing (VIGS) with Citrus tristeza virus (CTV), using the sense and antisense orientations. The antisense orientation showed a stronger reduction of GFP expression in N. benthamiana compared with the sense orientation. Similarly, the anti...

文献信息： Kanwar Jyoti, Kaul M. K., Naruka I. S., Singh P. P.等.  发表于【Indian Journal of Agricultural Sciences】 | 请登录

简介：This study was caned out to assess the potential use and applicability of micrografting technique for the development of virus free nursery in sweet orange (Citrus sinensis Osbeck) cv. Blood Red Malta. The micro grafting was carried out using 11-20 days old etiolated seedlings of Rough Lemon, Sour orange, Carrizo and Karna Khatta rootstocks. An apical meristem (1-2 mm) having 3-leaf primordial (0....

文献信息：Jia Ning, Liu Jiqin, Tan Penghui, Sun Yufeng, Lv Yuemeng, Liu Jiameng, Sun Jing, Huang Yatao, Lu Jia, Jin Nuo, Li Minmin, Imam Khandaker M, Xin Fengji等.  2019-02-25 发表于【Frontiers in Plant Science】 | 请登录

简介：Varieties of Citrus are commercially important fruits that are cultivated worldwide and are valued for being highly nutritious and having an appealing flavor. Lignification of citrus fruit juice sacs is a serious physiological disorder that occurs during postharvest storage, for which the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a c...

文献信息：Jia Hongge, Zou Xiuping, Orbovic Vladimir, Wang Nian等.  发表于【Methods in molecular biology (Clifton, N.J.)】 | 请登录

简介： CRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be created by Agrobacterium-mediated epicotyl transformation.
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文献信息：Jia Hongge, Orbovi Vladimir, Wang Nian等.  2019-03-25 发表于【Plant biotechnology journal】 | 请登录

简介： Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully in Duncan grapefruit via Xcc-facilitated agroinfiltration. Next, LbCas12a drive...

文献信息：Jain Mukesh, Munoz-Bodnar Alejandra, Gabriel Dean W.等.  发表于【Journal of Plant Physiology】 | 请登录

简介：The Lasbcp (CLIBASIA_RS00445) 1-Cys peroxiredoxin gene is conserved among all 13 sequenced strains of Candidatus Liberibacter asiaticus, the causal agent of Huanglongbing or "citrus greening" disease. LasBCP was previously characterized as a secreted peroxiredoxin with substrate specificity for organic peroxides, and as a potential pathogenicity effector. Agrobacterium-mediated transient expressio...

文献信息：Huang Ding, Yuan Yue, Tang Zhouzhou, Huang Yue, Kang Chunying, Deng Xiuxin, Xu Qiang等.  2019-07-15 发表于【 Plant, cell & environment】 | 请登录

简介：Blood orange is generally recognized to accumulate anthocyanins in its fruit pulp in a cold-inducible manner. We observed that the fruit peel of blood orange can also accumulate anthocyanins under ample light conditions. Interestingly, purple pummelo can accumulate anthocyanins only in its fruit peel but not in its pulp. The mechanism underlying the tissue specificity of anthocyanin accumulation i...

文献信息： He Yongrui, Jia Ruirui, Qi Jingjing, Chen Shanchun, Lei Tiangang, Xu Lanzhen, Peng Aihong, Yao Lixiao, Long Qin, Li Zhengguo, Li Qiang等.  2019-07-30 发表于【Gene】 | 请登录

简介：Genetic engineering approaches offer an alternative method to the citrus canker resistance breeding. The ethylene response factor (ERF) family is a member of families of transcription factors that are particular to plants and contribute significantly to biotic stress response and to plant growth. CsAP2-09 belongs to the citrus AP2/ERF transcription factor family. Initially, we proved the induction...

文献信息：邹修平, 范迪, 彭爱红, 何永睿, 许兰珍, 雷天刚, 姚利晓, 李强, 罗克明, 陈善春等.  请登录

简介：为了获得CsLOB1启动子较大片段删除的突变体,利用CRISPR/Cas9技术对CsLOB1启动子进行多位点编辑。通过在CsLOB1启动子的EBEPthA4区域及其上、下游设计不同的靶标位点,构建了2个植物表达载体pCas9CsLOB1:2sites和pCas9CsLOB1:3sites,分别对CsLOB1启动子同时进行2个位点和3个位点的编辑。测序结果表明pCas9CsLOB1:2sites和pCas9CsLOB1:3sites的基因编辑效率分别为64.7%和80.0%,突变体植株在2个sgRNA之间发生了DNA片段的删除。进一步的分...

文献信息：김호방等.  发表于【Applied Biological Chemistry】 | 请登录

简介：Many citrus cultivars have the polyembryony trait that develops many nucellar embryos alongside a single zygotic embryo in an individual seed by sporophytic apomixis. This unique botanical trait hinders citrus breeding by genetic hybridization and affects breeding efficiency and cost. Techniques to efficiently identify nucellar and zygotic individuals in citrus are still very limited. For a system...

文献信息：Geng Jingjing, Wei Tonglu, Wang Yue, Huang Xiaosan, Liu Ji-Hong等.  2019-07-19 发表于【Tree physiology】 | 请登录

简介： Basic helix-loop-helix (bHLH) family of transcription factors plays crucial role in regulating plant response to abiotic stress by targeting a large spectrum of stress-responsive genes. However, the physiological mechanisms underlying the transcription factors-mediated stress response are still poorly understood for most of the bHLH genes. In this study, transgenic pummelo (Citrus grandis) plants...

文献信息：Goulin Eduardo Henrique, Galdeano Diogo Manzano, Granato Lais Moreira, Matsumura Emilyn Emy, Dalio Ronaldo Jose Durigan, Machado Marcos Anton等.  2019-09-01 发表于【Microbiological research】 | 请登录

简介： Citrus crops have great economic importance worldwide. However, citrus production faces many diseases caused by different pathogens, such as bacteria, oomycetes, fungi and viruses. To overcome important plant diseases in general, new technologies have been developed and applied to crop protection, including RNA interference (RNAi) and clustered regularly interspaced short palindromic repeat (CRIS...

文献信息： de Lima Silva Caio Cesar, Shimo Hugo Massayoshi, de Felicio Rafael, Mercaldi Gustavo Fernando, Rocco Silvana Aparecida, Benedetti Celso Edua等.  2019-03-07 发表于【 Scientific Reports】 | 请登录

简介：Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that...