文献信息:Perez-Roman, Estela, Borreda, Carles, Lopez-Garcia Usach, Antoni and Talon, Manuel等. 请登录
简介:Most of the hundreds of citrus varieties are derived from spontaneous mutations. We characterized the dynamics of single-nucleotide mosaicism in a 36-yr-old clementine (Citrus xclementina hort. ex Tanaka) tree, a commercial citrus whose vegetative behavior is known in detail. Whole-genome sequencing identified 73 reliable somatic mutations, 48% of which were transitions from G/C to A/T, suggesting...
文献信息:Shi, Qiao-Fang, Long, Jian-Mei, Yin, Zhao-Ping, Jiang, Nan, Feng, Meng-Qi, Zheng, Bo, Guo, Wen-Wu and Wu, Xiao-Meng等. 请登录
简介:Key message Overexpression of miR171 restored SE competence in the recalcitrant citrus callus, and inhibition of miR171 function weakened SE competence in the strongly embryogenic citrus callus. Somatic embryogenesis (SE) is an important way of in vitro regeneration for plants. For perennial woody crops such as citrus, embryogenic callus is usually induced from unfertilized aborted ovules and wide...
文献信息:Li, Shao-jia, Liu, Sheng-chao, Lin, Xia-hui, Grierson, Donald, Yin, Xue-ren and Chen, Kun-song等. 请登录
简介:Heat stress is a major abiotic stress for plants, which can generate a range of biochemical and genetic responses. In 'Ponkan' mandarin fruit, hot air treatment (HAT) accelerates the degradation of citric acid. However, the transcriptional regulatory mechanisms of citrate degradation in response to HAT remain to be elucidated. Here, 17 heat shock transcription factor sequences were isolated, and d...
文献信息:Zhang, Yang, Ming, Ruhong, Khan, Madiha, Wang, Yue, Dahro, Bachar, Xiao, Wei, Li, Chunlong and Liu, Ji-Hong等. 请登录
简介: Plant ethylene-responsive factors (ERFs) play essential roles in cold stress response, but the molecular mechanisms underlying this process remain poorly understood. In this study, we characterized PtrERF9 from trifoliate orange (Poncirus trifoliata (L.) Raf.), a cold-hardy plant. PtrERF9 was up-regulated by cold in an ethylene-dependent manner. Overexpression of PtrERF9 conferred prominently enh...
文献信息:Song, Jie, Wu, Hao, He, Feng, Qu, Jing, Wang, Yue, Li, Chunlong and Liu, Ji-Hong等. 请登录
简介:C-repeat (CRT) binding factors (CBFs) are well known to act as crucial transcription factors that function in cold stress response. Arginine decarboxylase (ADC)- mediated putrescine (Put) biosynthesis has been reported to be activated in plants exposed to cold conditions, but it remains elusive whether CBFs can regulate ADC expression and Put accumulation. In this study, we show that cold upregula...
文献信息:Jia, Hongge, Omar, Ahmad A., Orbovic, Vladimir and Wang, Nian等. 请登录
简介: Citrus canker caused by Xanthomonas citri subsp. citri is one of the most devastating citrus diseases worldwide. Generating disease-resistant citrus varieties is considered one of the most efficient and environmentally friendly measures for controlling canker. X. citri subsp. citri causes canker symptoms by inducing the expression of canker susceptibility gene LOB1 via PthA4, a transcription acti...
文献信息:Dahro, Bachar, Wang, Yue, Khan, Madiha, Zhang, Yang, Fang, Tian, Ming, Ruhong, Li, Chunlong and Liu, Ji-Hong等. 请登录
简介: Invertase (INV)-mediated sucrose (Suc) hydrolysis, leading to the irreversible production of glucose (Glc) and fructose (Frc), plays an essential role in abiotic stress tolerance of plants. However, the regulatory network associated with the Suc catabolism in response to cold environment remains largely elusive. Herein, the cold-induced alkaline/neutral INV gene PtrA/NINV7 of trifoliate orange (P...
文献信息: Mesejo, Carlos, Marzal, Andres, Martinez-Fuentes, Amparo, Reig, Carmina, de Lucas, Miguel, Iglesias, Domingo J., Primo-Millo, Eduardo, Blazquez, Migu等. 请登录
简介:In Citrus, the response to environmental floral inductive signals is inhibited by the presence of developing fruits. The mechanism involves epigenetic activation of the CcMADS19 locus (FLC orthologue), encoding a floral repressor. To understand how this epigenetic regulation is reverted to allow flowering in the following season, we have forced precocious sprouting of axillary buds in fruit-bearin...
文献信息:Zhu, Congyi, Peng, Cheng, Qiu, Diyang and Zeng, Jiwu等. 请登录
简介:Citrus grandis 'Tomentosa', commonly known as 'Huajuhong' pummelo (HJH), is used in traditional Chinese medicine and can moisten the lungs, resolve phlegm, and relieve coughs. A spontaneous bud mutant, named R-HJH, had a visually attractive phenotype with red albedo tissue and red juice sacs. In this study, the content and composition of carotenoids were investigated and compared between R-HJH and...
文献信息:Cimen, Berken, Yesiloglu, Turgut, Donmez, Dicle, Kacar, Yildiz Aka and Ercisli, Sezai等. 请登录
简介:Background Turkey is one of the major exporters of mandarins in the Mediterranean region. Seedlessness in citrus, which is one of the most desired fruit quality traits, especially in fresh mandarin export markets, can be obtained via triploidy as in many fruit species. Triploid plants can be recovered by 2x x 2x hybridizations in citrus, as well as 2x x 4x and 4x x 2x crosses. Hence, we aimed to d...
文献信息: Hajeri, Subhas, Ng, James, Grosser, Jude and Vidalakis, Georgios等. 请登录
简介:Viroids are RNA-based infectious agents that are single-stranded, covalently closed circular, non-coding, and naked. Unlike RNA viruses, which at least encode proteins for replication, encapsidation, and movement, lack of protein-coding capacity of viroids makes them completely reliant on host for replication and movement. The high genetic diversity in viroids is believed to be due to the absence ...
文献信息:Soriano, Leonardo, Omar, Ahmad A. and Martinelli, Adriana Pinheiro等. 请登录
简介:Protoplasts are an attractive explant source for biotechnological tools widely used on citrus genetic improvement, such as somatic hybridization and direct genetic transformation. These delicate and responsive materials are subjected to cell proliferation induction and differentiation of somatic embryos which further regenerate into entire plants. The isolation of viable protoplasts followed by re...
文献信息:de Melo Souza, Joanne Moraes, de Oliveira, Carolina Rossi, da Rocha Tavano, Eveline Carla, Soriano, Leonardo and Martinelli, Adriana Pinheiro等. 请登录
简介:Somatic embryogenesis has been obtained in many citrus cultivars; however, the efficiency of the system is genotype dependent and culture synchronization is important to reach more efficient systems. In this chapter we present a detailed protocol of somatic embryogenesis induction from nucellar tissue and the use of an alternative method of callus sieving for culture synchronization and embryo pro...
文献信息:Zhang, Mingfei, Wang, Jinqiu, Liu, Ruilian, Liu, Hai, Yang, Hongbin, Zhu, Zhifeng, Xu, Rangwei, Wang, Pengwei, Deng, Xiuxin, Xue, Shaowu, Zhu, Feng an等. 请登录
简介:CsMYB96 plays a role in regulating both APQ-mediated water movement and synthesis of cuticular wax to increase tolerance of citrus fruit to water loss. A Citrus sinensis R2R3 MYB transcription factor (CsMYB96) has previously been shown to be strongly associated with the expression of many genes related to wax biosynthesis in the fruit. In this study, CsMYB96 was found to alleviate water loss by si...
文献信息: Pizzo, Jessica Santos, Silva, Jiuliane Martins, Silva Santos, Patricia Daniele, Visentainer, Jesui Vergilio and Santos, Oscar Oliveira等. 请登录
简介:Orange fruit is one of the most popular types of fruit in the world, and its juice is the main product of its processing. This study aimed to evaluate a simple, fast, and eco-friendly methodology, atmospheric solids analysis probe mass spectrometry (ASAP-MS), to assess the chemical profile of four oranges varieties (Valencia, Folha Murcha, Pera, and Iapar). The oranges' varieties were evaluated fo...
文献信息:He, Jiaxian, Xu, Yuantao, Huang, Ding, Fu, Jialing, Liu, Ziang, Wang, Lun, Zhang, Yin, Xu, Rangwei, Li, Li, Deng, Xiuxin and Xu, Qiang等. 请登录
简介:The citrus transcription factor TRIPTYCHON-LIKE connects regulation of fruit flavor and color by competing with MYB activators; it forms a regulatory loop balancing citric acid and proanthocyanidin accumulation. Deciphering the genetic basis of organoleptic traits is critical for improving the quality of fruits, which greatly shapes their appeal to consumers. Here, we characterize the citrus R3-MY...
文献信息:Feng, Meng-Qi, Lu, Meng-Di, Long, Jian-Mei, Yin, Zhao-Ping, Jiang, Nan, Wang, Peng-Bo, Liu, Yue, Guo, Wen-Wu and Wu, Xiao-Meng等. 请登录
简介:Somatic embryogenesis (SE) is a major regeneration approach for in vitro cultured tissues of plants, including citrus. However, SE capability is difficult to maintain, and recalcitrance to SE has become a major obstacle to plant biotechnology. We previously reported that miR156-SPL modules regulate SE in citrus callus. However, the downstream regulatory pathway of the miR156-SPL module in SE remai...
文献信息:Longhi, Talita Vigo, de Carvalho, Deived Uilian, Duin, Izabela Moura, da Cruz, Maria Aparecida and Leite Junior, Rui Pereira等. 请登录
简介:Huanglongbing (otherwise known as HLB or greening) is currently the most devastating citrus disease worldwide. HLB is primarily associated with the phloem-inhabiting bacterium 'Candidatus Liberibacter asiaticus' (CLas). Currently, there are no citrus species resistant to CLas. Genetic transformation is one of the most effective approaches used to induce resistance against plant diseases. Antimicro...
文献信息: Liang, Beibei, Wan, Shiguo, Ma, Qingling, Yang, Li, Hu, Wei, Kuang, Liuqing, Xie, Jingheng, Liu, Dechun and Liu, Yong等. 请登录
简介:Drought is one of the main abiotic stresses limiting the quality and yield of citrus. Cuticular waxes play an important role in regulating plant drought tolerance and water use efficiency (WUE). However, the contribution of cuticular waxes to drought tolerance, WUE and the underlying molecular mechanism is still largely unknown in citrus. 'Longhuihong' (MT) is a bud mutant of 'Newhall' navel orang...
文献信息:Mi, Lanfang, Ma, Dong, Lv, Shuping, Xu, Saibing, Zhong, Balian, Peng, Ting, Liu, Dechun and Liu, Yong等. 请登录
简介: A complex molecular regulatory network plays an important role in the development and ripening of fruits and leads to significant differences in apparent characteristics. Comparative transcriptome and sRNAome analyses were performed to reveal the regulatory mechanisms of fruit ripening in a spontaneous early-ripening navel orange mutant ('Ganqi 4', <i>C</i><i>itrus sinensis</i> L. Osbeck) and its...