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  •  1000    1 2 3 4 5 6 7 8 9 10 下一页 尾页
    1. 柠檬合子胚自交实生苗的鉴定及其对柑橘溃疡病的抗性初步评价

    文献信息:谭李梅,符红艳,徐静,朱志媚,胡哲,肖顺元,马先锋,邓子牛等.  发表于【分子植物育种】 | 请登录

    简介:枸橼C-05是抗溃疡病的柑橘种质资源,柠檬是枸橼的后代,其自交后代可能继承枸橼的抗性基因。为分析柠檬自交群体对柑橘溃疡病的抗感性规律,本研究建立了433株的‘尤力克’柠檬自交后代群体,并利用SSR分子标记对合子胚实生苗进行了鉴定,观察合子胚实生苗和珠心胚实生苗叶片形态学的差异,对所有自交后代离体注射接种柑橘溃疡病菌,初步评价了实生后代对柑橘溃疡病的抗病性差异。结果表明‘尤力克’柠檬存在多胚性,从300对SSR引物中筛选到...

    2. 柑橘溃疡病抗性相关转录因子CitMYB20的功能

    文献信息:姚利晓,范海芳,张庆雯,何永睿,许兰珍,雷天刚,彭爱红,李强,邹修平,陈善春等.  发表于【中国农业科学】 | 请登录

    简介:【目的】了解柑橘不同品种CitMYB20对柑橘溃疡病菌(Xanthomonas citri subsp. citri,Xcc)和外源植物激素的应答情况,评价转基因植株对柑橘溃疡病的抗性,验证CitMYB20的生物学功能。【方法】根据柑橘基因组序列设计引物,PCR法同源克隆柑橘溃疡病抗性品种金弹金柑(Fortunella japonica)和敏感品种枣阳小叶枳(Poncirus trifoliata)的CitMYB20基因序列和启动子序列,利用在线软件PlantCARE对启动子序列进行顺式作用元件预测。以针刺法对金...

    3. 柚新品种‘老哥金柚’

    文献信息:朱宏爱,王智课,朱晚爱,刘朋等.  发表于【园艺学报】 | 请登录

    简介:‘老哥金柚’是从‘安江早香柚’优良芽变中选育出的新品种。树姿开张,树势强,枝梢较密,坐果力强。果实卵圆形,果皮浅黄色,果面油胞突出,果肉脆嫩无渣,香气浓,平均单果质量约1 213.0 g。少核或无核,含可溶性固形物14.3%、可滴定酸0.32%、维生素C 0.468 mg · g~(-1)。10月中旬果实成熟。丰产、稳产,初果期产量约18 900 kg · hm~(-2),盛果期约47 100 kg · hm~(-2)。抗逆性强。

    4. A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata

    文献信息:Zhu Feng, Luo Tao, Liu Chaoyang, Wang Yang, Zheng Li, Xiao Xue, Zhang Mingfei, Yang Hongbin, Yang Wei, Xu Rangwei, Zeng Yunliu, Ye Junli, Xu Juan, Xu 等.  2020-06-20 发表于【Journal of Experimental Botany】 | 请登录

    简介:Although abscisic acid (ABA) is a vital regulator of fruit ripening and several transcription factors have been reported to regulate ABA biosynthesis, reports of the effect of ABA on citrus ripening and the regulation of its biosynthesis by a multiple-transcription-factor complex are scarce. In the present study, a systematic metabolic, cytological, and transcriptome analysis of an ABA-deficient m...

    5. Computational identification of conserved microRNAs and functional annotation of their target genes in Citrus limon Identification of microRNA in Citrus limon

    文献信息:Roy Suraj, Nath Durbba, Paul Prosenjit, Chakraborty Supriyo等.  发表于【South African Journal of Botany】 | 请登录

    简介:Citrus limon (lemon) is an important fruit crop of Rutaceae family with high nutritive value. The microRNAs (miRNAs) are a class of small RNAs, approximately 22 nucleotides long. They are evolutionary conserved and function at post-transcriptional level to regulate the expressivity of genes in both plants and animals. They have crucial roles in different biological and metabolic processes like gro...

    6. Silver compounds regulate leaf drop and improve in vitro regeneration from mature tissues of Australian finger lime (Citrus australasica)

    文献信息:Mahmoud Lamiaa M., Grosser Jude W., Dutt Manjul等.  发表于【Plant Cell Tissue and Organ Culture】 | 请登录

    简介:Key message The study evaluates the efficacy of various silver-containing compounds on their ability to effectively control ethylene production and allow for the development of an efficient tissue culture protocol for Finger lime. Finger lime is a small-leaved citrus native to Australia that is becoming increasingly popular worldwide. Finger lime is monoembryonic and cannot be propagated true to t...

    7. Overexpression of a new proline-rich protein encoding Gene CsPRP4 increases starch accumulation in Citrus

    文献信息:Ma Yanyan, WuTianli, ZhongGuangyan, Zheng Yongqiang等.  2020-01-27 发表于【Scientia Horticulturael】 | 请登录

    简介:Proline-rich proteins (PRPs) are a group of proteins with repeated proline/hydroproline residues that synthesize cell walls. Here, we identified CsPRP4, a new gene encoding a hybrid PRP, from Citrus sinensis. CsPRP4 showed preferential expression in leaves and significant upregulation when treated with exogenous ethylene. We used the Citrus sinensis (L.) Osbeck genome to clone the 1.5 kb CsPRP4 pr...

    8. CsWAKL08, a pathogen-induced wall-associated receptor-like kinase in sweet orange, confers resistance to citrus bacterial canker via ROS control and JA signaling

    文献信息:Li Qiang, Hu Anhua, Qi Jingjing, Dou Wanfu, Qin Xiujuan, Zou Xiuping, Xu Lanzhen, Chen Shanchun, He Yongrui等.  发表于【Horticulture Research】 | 请登录

    简介:Citrus bacterial canker (CBC) is a disease resulting from Xanthomonas citri subsp. citri (Xcc) infection and poses a grave threat to citrus production worldwide. Wall-associated receptor-like kinases (WAKLs) are proteins with a central role in resisting a range of fungal and bacterial diseases. The roles of WAKLs in the context of CBC resistance, however, remain unclear. Here, we explored the role...

    9. Some factors affecting the efficiency of hybrid embryo rescue in the 'Shiranuhi' Mandarin

    文献信息:Kim Misun, Kim Si Hyun, Kim Ho Bang, Park Young Chul, Song Kwan Jeong等.  发表于【Horticultural Science & Technology】 | 请登录

    简介:The 'Shiranuhi' mandarin has attractive characteristics such as a high soluble solid content, a lack of seeds, and a rind that is easy to peel. Thus, it has been utilized as a genetic resource in citrus breeding programs. However, it has a polyembryony trait that causes problems when producing sexual hybrids. Therefore, methods need to be established to distinguish between zygotic and nucellar pla...

    10. Unpaveling the cryptic genetic variation and population structure of the citrus genotypes with unknown origin

    文献信息:Abouzari Abouzar,Solouki Mahmood,Golein Behrouz, Fakher Barat Ali,Sabouri Atefeh, Dadras Ahmad Reza等.  发表于【Genetika-Belgrade】 | 请登录

    简介:The high diversity of Citrus genotypes can increase the probability of identifying new genetic makeups and even desirable traits. Hence, 76 genotypes with unknown origin and 34 known cultivars from several Citrus species were analyzed by SSR and AFLP markers. Overall, 326 alleles and 545 polymorphic bands were respectively scored for 28 SSR loci and 25 AFLP primer-enzyme combinations. The results ...

    11. Fast-track breeding system to introduce CTV resistance of trifoliate orange into citrus germplasm, by integrating early flowering transgenic plants with marker-assisted selection

    文献信息:Tomoko Endo,Hiroshi Fujii,Mitsuo Omura,Takehiko Shimada等.  发表于【BMC Plant Biology】 | 请登录

    简介:Background: Global warming will expand the range of new and invasive pathogens in orchards, and subsequently increase the risk of disease epidemics and economic losses. The development of new resistant plant varieties can help to reduce the impact of pathogens, however, the breeding speed can be extremely slow, due to the growth rates of the plants, and the availability of resistance genes. Citrus...

    12. International Journal of Molecular Sciences

    文献信息:Fu Xing-Zheng, Zhou Xue, Xu Yuan-Yuan, Hui Qiu-Ling, Chun Chang-Pin, Ling Li-Li, Peng Liang-Zhi等.  2020-04-01 发表于【International Journal of Molecular Sciences】 | 请登录

    简介:Autophagy is a highly conserved intracellular degradation pathway that breaks down damaged macromolecules and/or organelles. It is involved in plant development and senescence, as well as in biotic and abiotic stresses. However, the autophagy process and related genes are largely unknown in citrus. In this study, we identified 35 autophagy-related genes (CsATGs-autophagy-related genes (ATGs) of Ci...

    13. Analysis of crossover events and allele segregation distortion in interspecific citrus hybrids by single pollen genotyping

    文献信息:Garavello, Miguel Cuenca, Jose Dreissig, Steven Fuchs, Joerg Navarro, Luis Houben, Andreas Aleza, Pablo等.  2020-05-25 发表于【Frontiers in Plant Science】 | 请登录

    简介:In citrus, a classical method of studying crossovers and segregation distortion (SD) is the genetic analysis of progenies. A new strategy combining fluorescence-activated cell sorting and whole genome amplification of haploid pollen nuclei with a large set of molecular markers, offers the opportunity to efficiently determine the frequency of crossovers and the identification of SD without the need...

    14. Phylogenetic relationships among accessions in Citrus and related genera based on the insertion polymorphism of the CIRE1 retrotransposon

    文献信息:Horibata Akira, Kato Tsuneo等.  发表于【Open Agriculture】 | 请登录

    简介:A total of 145 accessions of the genus Citrus and related genera, maintained in the Conservation Garden for Citrus Germplasm at the Experimental Farm of Kindai University, Yuasa, Wakayama, Japan, were examined for their phylogenetic relationships. The present classification was conducted using an inter-retrotransposon amplified polymorphism (IRAP) method based on the insertion polymorphism of a re...

    15. Establishment of an in vitro regeneration system and genetic transformation of the Tunisian 'Maltese half-blood' (Citrus sinensis): an agro-economically important variety

    文献信息:Jardak Rahma, Boubakri Hatem, Zemni Hassene, Gandoura Samia, Mejri Samiha, Mliki Ahmed, Ghorbel Abdelwahed等.  2020-02-06 发表于【3 Biotech】 | 请登录

    简介:An efficient in vitro regeneration system using epicotyl segments was developed and then used for optimizing genetic transformation of the Tunisian 'Maltese half-blood' (Citrus sinensis) variety using phosphinothricin (PPT) resistance as a selectable marker. The maximum regeneration efficiency was achieved after incubating epicotyl explants (excised in an oblique manner) in MT culture media contai...

    16. Generation of homozygous canker-resistant citrus in the T0 generation using CRISPR-SpCas9p

    文献信息:Jia Hongge, Wang Nian等.  发表于【Plant Biotechnology Journal】 |  , 请登录

    17. Evaluation of seedlings from 11 citrus accessions for in vivo micrografting

    文献信息:Le Minh-Ly, Sakai Kaori, Mizunoe Yuki, Ozaki Yukio, Wakana Akira等.  发表于【Horticulture Journal】 | 请登录

    简介:The very young seedlings of trifoliate orange, and its hybrids with trifoliate leaves as a marker, are usually used as a rootstock for in vitro and in vivo micrografting of citrus to eliminate viruses in spring in temperate regions. In tropical and subtropical regions or in summer, however, the production and use of trifoliate orange seedlings is difficult. Therefore, it is necessary to establish ...

    18. ClPLD5, a phospholipase gene is involved in protection of cytoskeleton stability in pollen of self-imcompatible 'Xiangshui' lemon (Citrus limon)

    文献信息:Lin Wei, Minhphu Do, Wei Mi, He Xinhua, Liu Yuan, Fan Zhiyi, Zhang Shuwei, Luo Cong, Huang Guixiang, Hu Guibing等.  发表于【Plant Cell Tissue and Organ Culturerrk7】 | 请登录

    简介:AC2-PLD gene (ClPLD5) related to self-incompatibility was identified within the "Xiangshui" lemon C2-PLD family, and overexpression of the ClPLD5 gene in pollen increased the stability of the pollen cytoskeleton under self-incompatibility challenge. Self-incompatibility (SI) is an important mechanism for the prevention of self-fertilization in plants and is one of the major causes of seedlessness ...

    19. Up-regulation of microRNA targets correlates with symptom severity in Citrus sinensis plants infected with two different isolates of citrus psorosis virus

    文献信息:Marmisolle Facundo E., Arizmendi Ailin,Ribone Andres, Rivarola Maximo, Garcia Maria L., Reyes Carina A.等.  发表于【Planta】 | 请登录

    简介:Sweet orange (Citrus sinensis) may suffer from disease symptoms induced by virus infections, thus resulting in drastic economic losses. Infection of sweet orange plants with two isolates of citrus psorosis virus (CPsV), expressing different symptomatologies, alters the accumulation of a set of endogenous microRNAs (miRNAs). Here, we predicted ten putative targets from four down-regulated miRNAs: t...

    20. Substantial equivalence of a transgenic Lemon fruit showing postharvest fungal pathogens resistance

    文献信息:Muccilli Vera, Vitale Alessandro, Sheng Ling, Gentile Alessandra, Cardullo Nunzio, Tringali Corrado, Oliveri Cinzia, La Rosa Rosa, Di Guardo Mario, La等.  2020-03-25 发表于【Journal of Agricultural and Food Chemistry】 | 请登录

    简介:The development of genetically modified (GM) crops speeds up the obtainment of novel varieties with improved agronomic characteristics. However, the risk evaluation of the use of GMs is mandatory before their release in the market. In this paper, an untargeted and comprehensive nuclear magnetic resonance-based metabolomic study was carried out on the peel and flesh of a transgenic lemon clone (E23...

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