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    1. Reaction of transgenic Citrus sinensis plants to Citrus tristeza virus infection by Toxoptera citricida

    文献信息:Muniz Fabiana R,Muniz, Fabiana R.,Harakava, Ricardo,Alves Mourao Filho, Francisco de Assis,Stach-Machado, Dagmar R.,Rezende, Jorge A. M.,Febres, Vicen等.  2014-01-09 发表于【European Journal of Plant Pathology】 |  , 请登录

    简介:Transgenic Citrus sinensis \'Hamlin\' and \'Valencia\' plants containing Citrus tristeza virus (CTV)-derived sequences were propagated and inoculated with CTV. For propagation, selected buds from transgenic and non-transgenic control plants were grafted onto C. aurantium and C. limonia rootstock plants. CTV inoculation was performed via viruliferous aphids (Toxoptera citricida), and viral detectio...

    2. Expression of the citrus CsTIP2;1 gene improves tobacco plant growth, antioxidant capacity and physiological adaptation under stress conditions

    文献信息:Martins, C. P. Neves, D. M. Cidade, L. C. Mendes, A. F. Silva, D. C. Almeida, A. F. Coelho-Filho, M. A. Gesteira, A. S. Soares-Filho, W. S. Costa, M. 等.  2017-01-21 发表于【Planta】 |  , 请登录

    简介:Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins, belonging to the major intrinsic protein family. In a previous study, we have shown that a citrus TIP isoform, CsTIP2;1, is highly expressed in leaves and also transcriptionally regulated in leaves and roots by salt and drought stresses and infection by ‘Candidatus Liberibacter asiaticus’, the causal agent of the Huanglongbing di...

    3. 柑橘果实特异表达基因CsPMEI1在拟南芥和金柑中的过量表达研究

    文献信息:张红红等.   , 请登录

    4. Accurate measurement of transgene copy number in crop plants using droplet digital PCR

    文献信息:Collier, Ray Dasgupta, Kasturi Xing, Yan‐\Ping Hernandez, Bryan Tarape Shao, Min Rohozinski, Dominica Kovak, Emma Lin, Jeanie De Oliveira, Maria Luiza等.  2017-02-23 发表于【Plant Journal】 |  , 请登录

    简介:Technical abstract: Genetic transformation is a powerful means for the improvement of crop plants, but requires labor and resource intensive methods. An efficient method for identifying single copy transgene insertion events from a population of independent transgenic lines is desirable. Currently transgene copy number is estimated by either Southern blot hybridization analyses or quantitative pol...

    5. Ectopic accumulation of linalool confers resistance to Xanthomonas citri subsp citri in transgenic sweet orange plants

    文献信息:Shimada, Takehiko Endo, Tomoko Rodriguez, Ana Fujii, Hiroshi Goto, Shingo Matsuura, Takakazu Hojo, Yuko Ikeda, Yoko Mori, Izumi C. Fujikawa, Takashi P等.  2017-06-28 发表于【Tree Physiology】 |  , 请登录

    简介:In order to clarify whether high linalool content in citrus leaves alone induces strong field resistance to citrus canker caused by Xanthomonas citri subsp. citri (Xcc), and to assess whether this trait can be transferred to a citrus type highly sensitive to the bacterium, transgenic \'Hamlin\' sweet orange (Citrus sinensis L. Osbeck) plants over-expressing a linalool synthase gene (CuSTS3-1) were...

    6. Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

    文献信息:Peng, Aihong Chen, Shanchun Lei, Tiangang Xu, Lanzhen He, Yongrui Wu, Liu Yao, Lixiao Zou, Xiuping等.  2017-03-21 发表于【Plant biotechnology journal】 |  , 请登录

    简介:Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistance through CRISPR/Cas9-targeted modification of the susceptibility gene CsLOB1 pro...

    7. 柑橘新品种‘桂野生山金柑’的选育

    文献信息:李果果, 麦彩胜 ,刘要鑫, 柴利军 ,陈香玲 ,赵小龙, 欧智涛, 张兰 ,赵洪涛,等.  2017-03-01 发表于【果树学报】 |  , 请登录

    简介:‘桂野生山金柑’是在广西壮族自治区东兴市发现的金柑属柑橘新品种。果实近圆形,成熟时果皮黄色,果面光滑,有蜡层,肉质紧密,风味显酸,平均单果质量4.5 g,果形指数0.98,种子1~7粒,卵形,深绿色,单胚。果实可食率87.1%,果实可溶性固形物含量10.6%,每L果汁中含可滴定酸45 g和维生素C 374 mg。该品种在广西东兴地区11月初成熟,花单生或簇生,萌芽力和成枝力弱,抗逆性和抗病性中等。适合广西地区栽培,幼树第2年可开花坐果。

    8. 柑橘砧木新品种‘蒲江香橙’的选育

    文献信息:伏晓科, 黄晓姣, 陈涛, 张静 ,王燕, 陈清, 雷清良 ,汤浩茹, 王小蓉等.  2017-05-01 发表于【果树学报】 |  , 请登录

    简介:蒲江香橙’是从四川省蒲江县白云乡实生野生香橙中经过多年观察和试验比较选择育成的柑橘砧木新品种。作为砧木,该品种树势强健,树冠圆头形,植株高大,枝条较硬、直立而密生、伸展。果实椭圆近扁圆形,果皮橙黄色,单果质量78.1~112.8 g,单果种子数32~43粒,平均37.5粒,种子质量占果实的10.1%左右,盛年期每株树可产种子3.5~5.5 kg。用作柑橘砧木,嫁接亲和性好,接穗品种早结、丰产、稳产,果实品质优良,遗传性状稳定。抗寒、抗涝、耐碱性土...

    9. Induction of haploid plants in citrus through gamma-irradiated pollen and ascertainment of ovule age for maximum recovery of haploid plantlets

    文献信息:Kundu, Manoj Dubey, Anil Srivastav, Manish Malik, Surendra等.  2017-06-14 发表于【Turkish Journal of Biology】 |  , 请登录

    简介:The present investigation was carried out for the induction of haploid plants in Citrus grandis through in situ parthenogenesis by pollination with gamma-irradiated pollen of C. limetta and C. sinensis, treated with 50, 100, 200, 300, and 400 Gy gamma ray doses followed by in vitro ovule culture. Ovule culture at 50 days after pollination (DAP) was found optimum for the maximum recovery of in vitr...

    10. Kanamycin selection in temporary immersion bioreactors allows visual selection of transgenic citrus shoots

    文献信息:Acanda, Yosvanis Canton, Michel Wu, Hao Zale, Janice等.  2017-05-10 发表于【Plant Cell Tissue & Organ Culture】 |  , 请登录

    简介:In mature citrus transformation, the nptII gene is most commonly used for selection and it is confounded by the high number of non-transformed, escaped shoots that develop on semi-solid kanamycin selection medium, even at high concentrations. Selection in liquid medium with kanamycin in temporary immersion bioreactors might provide a better means of distinguishing between transformed and non-trans...

    11. NMR-based metabolomics of transgenic and non-transgenic sweet orange reveals different responses in primary metabolism during citrus canker development

    文献信息:Apparecido, Rafael do Prado Carlos, Eduardo Fermino Liao, Luciano Morais Esteves Vieira, Luiz Gonzaga Alcantara, Glaucia Braz等.  2017-07-18 发表于【Metabolomics】 |  , 请登录

    简介:Introduction Citrus canker, a disease caused by Xanthomonas axonopodis pv. citri (Xac) bacteria, has been responsible for extensive economic losses in citriculture. In this work, we report the metabolic responses of citrus plants during disease development. This information can be useful for understanding the natural mechanism of plant defense beyond helping design new varieties and/or genetically...

    12. Production of three new grapefruit cybrids with potential for improved citrus canker resistance

    文献信息:Omar, Ahmad A. Murata, Mayara Yu, Qibin Gmitter, Fred G. Chase, Christine D. Graham, James H. Grosser, Jude W.等.  2017-07-27 发表于【In Vitro Cellular & Developmental Biology - Plant】 |  , 请登录

    简介:Cybrid production, combining the nucleus of one species with alien cytoplasmic organelles of another, is a potentially valuable method used for improvement of various crops including Citrus species. Furthermore, this technology is considered a non-GMO biotechnology strategy. In citrus, cybrid plants can be produced as a by-product of somatic fusion. Host resistance is the most desirable strategy f...

    13. Regulatory cis-elements on citrus peel-specific expressed gene, CuCRTISO-like, promoter respond to hormones and abiotic stresses in transgenic Arabidopsis

    文献信息:Eun, Chang-Ho Kim, Seong- U. Kim, In-Jung等.  2017-03-15 发表于【Plant Biotechnology Reports】 |  , 请登录

    简介:We identified a peel-specific expressed gene in Citrus unshiu fruits by differentially expressed gene (DEG) analysis, which showed a homology with carotenoid isomerase-like genes identified from other plants and, therefore, designated as CuCRTISO-like. Here we determined the promoter sequence of CuCRTISO-like and analyzed histochemical GUS activity using transgenic Arabidopsis plants harboring CuC...

    14. Transgenic citrus plants expressing the p35 anti-apoptotic gene have altered response to abiotic stress

    文献信息:Orbovic, Vladimir Fields, Jeb S. Syvertsen, James P.等.  发表于【Horticulture Environment and Biotechnology】 |  , 请登录

    简介:Transgenic plants of the citrus rootstock Carrizo citrange cultivar [Citrus sinensis (L.) Osbeck x Poncirus trifoliata (L.) Raf.] expressing a p35 anti-apoptotic gene were exposed to three types of abiotic stress. The responses of plants from the three transgenic lines, p35-1, p35-2, and p35-4 were compared to those of wild-type (WT) plants, revealing consistent improvements in the ability of leaf...

    15. 柑橘新品种‘桂野生山金柑’的选育

    文献信息:李果果 ,麦彩胜 ,刘要鑫 ,柴利军, 陈香玲 ,赵小龙, 欧智涛, 张兰 赵洪涛等.  发表于【果树学报】 | 请登录

    简介:桂野生山金柑’是在广西壮族自治区东兴市发现的金柑属柑橘新品种。果实近圆形,成熟时果皮黄色,果面光滑,有蜡层,肉质紧密,风味显酸,平均单果质量4.5 g,果形指数0.98,种子1~7粒,卵形,深绿色,单胚。果实可食率87.1%,果实可溶性固形物含量10.6%,每L果汁中含可滴定酸45 g和维生素C 374 mg。该品种在广西东兴地区11月初成熟,花单生或簇生,萌芽力和成枝力弱,抗逆性和抗病性中等。适合广西地区栽培,幼树第2年可开花坐果。

    16. 柑橘砧木新品种‘蒲江香橙’的选育

    文献信息:伏晓科 ,黄晓姣, 陈涛, 张静,王燕, 陈清 ,雷清良, 汤浩茹 ,王小蓉等.  发表于【果树学报】 | 请登录

    简介:‘蒲江香橙’是从四川省蒲江县白云乡实生野生香橙中经过多年观察和试验比较选择育成的柑橘砧木新品种。作为砧木,该品种树势强健,树冠圆头形,植株高大,枝条较硬、直立而密生、伸展。果实椭圆近扁圆形,果皮橙黄色,单果质量78.1~112.8 g,单果种子数32~43粒,平均37.5粒,种子质量占果实的10.1%左右,盛年期每株树可产种子3.5~5.5 kg。用作柑橘砧木,嫁接亲和性好,接穗品种早结、丰产、稳产,果实品质优良,遗传性状稳定。抗寒、抗涝、耐碱性...

    17. 利用转座子显示技术鉴别柑橘芽变品种

    文献信息:柯玲俊, 余惠文, 许海丹, 谢宗周, 邓秀新 ,徐强等.  发表于【园艺学报】 |  , 请登录

    简介:在依据柑橘全基因组信息的基础上,针对转座子序列开发了一种适合鉴别柑橘芽变品种的分子技术。以43个柑橘芽变品种和7个普通柑橘品种为材料,采用转座子显示技术(transposon display,TD)对转座子进行多态性分析。结果表明:5个DNA转座子(DTM63、DTM58、Helitron1、Spem和mPing2)和4个反转录转座子(Line1、LTR-Gypsy3、LTR-Gypsy5和Tcs2)可以较好地鉴别柑橘不同芽变品种。罗伯逊脐橙及其早熟芽变品种罗脐35号能被DTM63、Spem和mPing2鉴别...

    18. 茂谷柑和W·默科特引种表现及品质特性

    文献信息:阳艳华 ,邓立宝 ,刘翠燕 ,杨之湜等.  发表于【浙江农业科学】 | 请登录

    简介:为了解晚熟柑橘品种茂谷柑和W·默科特在广西桂林市的种植表现,引进茂谷柑和W·默科特品种并进行试种和调查分析。结果表明,茂谷柑在广西桂林市2月下旬至3月上旬果实成熟,平均单果质量153.63 g,果实可溶性固形物和可溶性糖含量分别为14.61%和10.09%;定植4年后平均单株产量达34.14 kg,产量和产值分别为45 065 kg·hm~(-2)和36.05万元·hm~(-2)。W·默科特在2月中旬成熟,平均单果质量为160.21 g,果实可溶性固形物和可溶性糖含量分别为1...

    19. 葡萄柚新品种‘桂葡萄柚二号’的选育

    文献信息:杨杰 ,王博 ,杨昌鹏 ,许立明, 赵小龙等.  2017-02-02 发表于【果树学报】 |  , 请登录

    简介:‘桂葡萄柚二号’是由‘红马叙’葡萄柚芽变单株选育而成。果实扁圆形,成熟期果皮不套袋为黄绿色,套袋后呈粉红色;果面光滑,果肉和囊瓣均为粉红色;果肉细嫩化渣,酸甜苦味适中,具有浓郁的葡萄柚风味。平均单果质量350 g,最大单果质量390 g;果形优美,果形指数0.87;每果实含种子3~5粒。果汁率55.7%,可溶性固形物含量10.1%,可食率79.41%,总糖55 g·L-1,总酸0.8%,糖酸比6.87,维生素C含量325 mg·kg~(-1),品质上乘;产量高,株产52.5 kg。果实...

    20. NMR-based metabolomics of transgenic and non-transgenic sweet orange reveals different responses in primary metabolism during citrus canker development

    文献信息:Rafael do Prado Apparecido1 , Eduardo Fermino Carlos2 , Luciano Morais Lião3 , Luiz Gonzaga Esteves Vieira4 , Glaucia Braz Alcantara1等.  2017-01-18 发表于【Metabolomics】 |  , 请登录

    简介:Introduction Citrus canker, a disease caused by Xan-thomonas axonopodis pv. citri (Xac) bacteria, has been responsible for extensive economic losses in citriculture.In this work, we report the metabolic responses of citrus plants during disease development. This information can be useful for understanding the natural mechanism of plantdefense beyond helping design new varieties and/or geneti-cally...

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