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  •  978    1 2 3 4 5 6 7 8 9 10 下一页 尾页
    1. Reaction of transgenic Citrus sinensis plants to Citrus tristeza virus infection by Toxoptera citricida

    文献信息:Muniz Fabiana R,Muniz, Fabiana R.,Harakava, Ricardo,Alves Mourao Filho, Francisco de Assis,Stach-Machado, Dagmar R.,Rezende, Jorge A. M.,Febres, Vicen等.  2014-01-09 发表于【European Journal of Plant Pathology】 |  , 请登录

    简介:Transgenic Citrus sinensis \'Hamlin\' and \'Valencia\' plants containing Citrus tristeza virus (CTV)-derived sequences were propagated and inoculated with CTV. For propagation, selected buds from transgenic and non-transgenic control plants were grafted onto C. aurantium and C. limonia rootstock plants. CTV inoculation was performed via viruliferous aphids (Toxoptera citricida), and viral detectio...

    2. 我国近十年审(认)定的柑桔新品种及其解读脐橙早熟新品种‘赣脐4号’的选育

    文献信息:伊华林, 李秋景, 程玉芳, 米兰芳, 汪爱云, 钟八莲, 陈健美, 苏华楠, 彭婷, 钟日芬, 林石鑫等.  发表于【中国南方果树】 |  , 请登录

    简介: 近年来,我国柑桔育种取得了较大进展。对2006 — 2014年我国审(认)定的柑桔新品种
    <br />数量、类型,涉及的育种技术、人员和单位情况进行整理。结果显示,此期间,我国育种队伍不断壮
    <br />大,核心力量逐渐形成,育种手段多样化程度有所提高;共审(认)定113个新品种,其中引种41
    <br />个,芽变选种(含珠心系变异) 53个等,以异源四倍体体细胞杂种为父本再和二倍体杂交获得三倍
    <br />体品种1个。同时,...

    3. 转基因柑橘外源基因拷贝数的实时荧光定量PCR检测

    文献信息:许兰珍, 何永睿, 雷天刚, 彭爱红, 姚利晓, 姜国金, 李强, 邹修平, 陈善春等.  发表于【园艺学报】 |  , 请登录

    简介:为分析转基因柑橘中外源基因整合拷贝数,基于实时荧光定量 PCR 法,建立通用、准确、
    <br />简便的转基因柑橘外源基因拷贝数检测体系,利用该体系成功检测了 150 个转基因柑橘单株系外源基因
    <br />整合拷贝数。结果表明,转基因柑橘中外源基因整合最低拷贝数为 1,最高达 5 个,其中 1、2 和 ≥ 3
    <br />拷贝的单株数分别占总数的 40.0%、18.0%和 42.0%;采用 Southern blot 技术进行检测,结果基本一致,
    <br />极少数单株经实时...

    4. 柑橘新品种‘金峡桃叶橙’

    文献信息:吴黎明, 蒋迎春, 何利刚, 王志静, 仝铸, 孙中海, 许淼, 吴述勇, 廖胜才等.  发表于【园艺学报】 |  , 请登录

    简介:‘金峡桃叶橙’是从‘桃叶橙 18 号’芽变中选育出的新品种。树体生长健壮,果实近圆球形,橙红色,果形指数 0.98,平均单果质量 140 g 以上。果肉橙黄色,少核或无核,高糖低酸,香气浓郁,质脆化渣,风味香甜。果汁含可溶性固形物 12.96%,可滴定酸 0.45%,维生素 C 304.5 mg · L -1 ,品质优。早结实,丰产稳产性好,发梢能力强,在湖北秭归地区 4 月中下旬开花,11 月下旬至 12 月上旬果实成熟。

    5. 脐橙早熟新品种‘赣脐4号’的选育

    文献信息:米兰芳, 汪爱云, 钟八莲, 陈健美, 苏华楠, 彭婷, 钟日芬, 林石鑫等.  发表于【果树学报】 |  , 请登录

    简介:‘赣脐4号’是从‘纽荷尔’脐橙芽变中选育的脐橙新品种。该品种果实圆形或偏扁圆形,单果质量220~240 g,成熟果实外观漂亮,品质优,果肉脆嫩化渣,易剥皮,可溶性固形物含量12.1%,总酸含量0.47%,维生素C含量633.0mg·L -1 。树势中等,坐果率高,早结丰产性好。多点区域试验和品种比较试验表明,该品种成熟早,成熟期在9月下旬至10月上旬。

    6. 柑橘新品种‘桂橘一号’的选育

    文献信息:邓崇岭, 陈传武, 唐艳, 伊华林, 邓光宙, 付慧敏, 刘冰浩, 罗鸿林, 范承彪等.  发表于【果树学报】 |  , 请登录

    简介:‘桂橘一号’是从‘蜜广橘’芽变中选育的早熟柑橘新品种。果实扁圆形,果皮橙黄色,果面光滑,有光泽,果肉橙黄色,化渣,味清甜;平均单果质量46.77 g,最大单果质量63.3 g,果形指数0.72;每果实含种子0.85粒,单胚,子叶淡绿色。可食率78.01%,果汁率61.31%,可溶性固形物含量12.29%,全糖11.02%,可滴定酸0.64%,固酸比19.85,维生素C含量212.0 mg·g -1 ;品质上等。果实生育期160 d,在桂林兴安 10月上中旬成熟。桂北地区及...

    7. QTL mapping of mandarin (Citrus reticulata) fruit characters using high-throughput SNP markers

    文献信息:Yu, Y., Chen, C. X., Gmitter, F. G.等.  发表于【Tree Genetics & Genomes】 | 请登录

    简介:Seedlessness, flavor, and color are top priorities for mandarin (Citrus reticulata Blanco) cultivar improvement. Given long juvenility, large tree size, and high breeding cost, marker-assisted selection (MAS) may be an expeditious and economical approach to these challenges. The objectives of this study were to construct high-density mandarin genetic maps and to identify single nucleotide polymorp...

    8. Factors affecting Agrobacterium-mediated transformation efficiency of kumquat seedling internodal stem segments

    文献信息:Yang, L., Hu, W., Xie, Y. M., Li, Y., Deng, Z. N.等.  发表于【Scientia Horticulturae】 |  , 请登录

    简介:Kumquat (Fortunella crassifolia Swingle.) has a short juvenile phase of 2-3 years and it can flower and bear fruit several times per year. It is, therefore, helpful for citrus to evaluate functional genes rapidly. In the present experimentation, a comprehensive and systematic study was performed to improve the transformation efficiency of kumquat, which was usually less than 4.1% in previous repor...

    9. Biolistic transformation of Carrizo citrange (Citrus sinensis Osb. x Poncirus trifoliata L. Raf.)

    文献信息:Wu, H., Acanda, Y., Jia, H. G., Wang, N. A., Zale, J.等.  发表于【Plant Cell Reports】 | 请登录

    简介:The development of transgenic citrus plants by the biolistic method. A protocol for the biolistic transformation of epicotyl explants and transgenic shoot regeneration of immature citrange rootstock, cv. Carrizo (Citrus sinensis Osb. x Poncirus trifoliata L. Raf.) and plant regeneration is described. Immature epicotyl explants were bombarded with a vector containing the nptII selectable marker and...

    10. Induction of parthenogenetic haploid plants using gamma irradiated pollens in \'Hirado Buntan\' pummelo (Citrus grandis L. Osbeck)

    文献信息:Wang, S. M., Lan, H., Jia, H. H., Xie, K. D., Wu, X. M., Chen, C. L., Guo, W. W.等.  发表于【Scientia Horticulturae】 |  , 请登录

    简介:Homozygous genotypes have great potential in breeding and genomic researches in higher plants. Induction of parthenogenesis via pollination with gamma-irradiated pollens is an efficient method to obtain haploid plants that can overcome the biological limitations of fruit trees. In this study, we investigated the induction of haploid plants in ‘Hirado Buntan’ pummlo (Citrus grandis) through in-si...

    11. Species discrimination of the genus Citrus and the hybridization analysis

    文献信息:Sun, Y. L., Jin, C. W., Hong, S. K.等.  发表于【Research Journal of Biotechnology】 | 请登录

    简介:The genus Citrus (Aurantioideae, Rutaceae) is the most important fruit crops all around the world. As many cultivated and weedy species/varieties exist in this genus, botanists hold different opinions on the taxonomy and phylogeny of the genus Citrus all the time. To evaluate the taxonomy and phylogenetic relationship of Citrus species, we collected 22 Citrus species and evaluated the genetic dive...

    12. A survey of FLS2 genes from multiple citrus species identifies candidates for enhancing disease resistance to Xanthomonas citri ssp. citri

    文献信息:Shi, Q. C., Febres, V. J., Jones, J. B., Moore, G. A.等.  发表于【Horticulture Research】 |  , 请登录

    简介:Pathogen-associated molecular patterns (PAMPs)-triggered immunity (PTI) is an important component of plant innate immunity. In a previous study, we showed that the PAMP flg22 from Xanthomonas citri ssp. citri (Xflg22), the causal agent of citrus canker, induced PTI in citrus, which correlated with the observed levels of canker resistance. Here, we identified and sequenced two bacterial flagellin/f...

    13. Effects of salinity on diploid (2x) and doubled diploid (4x) Citrus macrophylla genotypes

    文献信息:Ruiz, M., Quinones, A., Martinez-Alcantara, B., Aleza, P., Morillon, R., Navarro, L., Primo-Millo, E., Martinez-Cuenca, M. R.等.  发表于【Scientia Horticulturae】 |  , 请登录

    简介:Tetraploid (4x) citrus seedlings may be more tolerant to salt stress than diploid (2x) genotypes. Genome duplication in citrus changes both plant physiology and anatomy, leading plants to acquire differentiated capacities to uptake and transport mineral elements. To provide insight into this behaviour, 2x and 4x Citrus macrophylla (CM), seedlings were grown at moderate (40 mM NaCl) and high salini...

    14. Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing

    文献信息:Reyes, C. A., De Francesco, A., Ocolotobiche, E. E., Costa, N., Garcia, M. L.等.  发表于【Physiological and Molecular Plant Pathology】 |  , 请登录

    简介:Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection w...

    15. Citrus breeding, genetics and genomics in Japan

    文献信息:Omura, M., Shimada, T.等.  发表于【Breeding Science】 |  , 请登录

    简介:Citrus is one of the most cultivated fruits in the world, and satsuma mandarin (Citrus unshiu Marc.) is a major cultivated citrus in Japan. Many excellent cultivars derived from satsuma mandarin have been released through the improvement of mandarins using a conventional breeding method. The citrus breeding program is a lengthy process owing to the long juvenility, and it is predicted that marker-...

    16. Production of Three New Grapefruit Like Cybrids Confirmed by Plant Mitochondrial Intron, Chloroplast and Nuclear DNA Markers to Improve Citrus Canker Resistance

    文献信息:Omar, A. A., Murata, M., Yu, Q. B., Gmitter, F. G., Chase, C. D., Graham, J. H., Grosser, J. W.等.  发表于【In Vitro Cellular & Developmental Biology-Animal】 | 请登录

    简介:The production of cybrids, combining nucleus of a species with alien cytoplasmic organelles, is a valuable method used for improvement of various crops. Moreover, this technology considers a non-GMO biotechnology strategy. In citrus, Cybrid plants can be produced as a by-product of somatic fusion. Several citrus cybrids have been created by somatic hybridization. Through the cell fusion between nu...

    17. Screening of \'King\' mandarin (Citrus nobilis Lour) x Poncirus trifoliata ((L.) Raf.) hybrids as citrus rootstocks tolerants to iron chlorosis

    文献信息:Martinez-Cuenca, M. R., Quinones, A., Forner-Giner, M. A.等.  发表于【Scientia Horticulturae】 |  , 请登录

    简介:Iron (Fe) chlorosis is one of the commonest problems in calcareous soils and plant tolerance is deter-
    <br />mined mainly by rootstocks. This work evaluates new citrus hybrids’ tolerance to Fe chlorosis compared with the most widely used rootstocks in Spain: Citrus macrophylla (CM, Fe chlorosis-tolerant) and Carrizo citrange (CC, Fe chlorosis-sensitive). Growth parameters, Fe concentration, photo...

    18. Induction of somatic embryogenesis in woody plants

    文献信息:Isah, T.等.  发表于【Acta Physiologiae Plantarum】 | 请登录

    简介:Somatic embryogenesis, the in vitro developmental program by which somatic cells are reprogrammed to undergo cellular and molecular changes that make them competent to produce somatic embryos, has been achieved with many woody plants. The program involves the stages of competence acquisition, induction and expression of the morphogenic pathway by the cultured cells and tissues. The ability to expr...

    19. Single-pollen genotyping to estimate mode of unreduced pollen formation in Citrus tamurana cv. Nishiuchi Konatsu

    文献信息:Honsho, C., Sakata, A., Tanaka, H., Ishimura, S., Tetsumura, T.等.  发表于【Plant Reproduction】 | 请登录

    简介:2n pollen formed by FDR in citrus. The Japanese local citrus cultivar, Nishiuchi Konatsu (Citrus tamurana hort. ex Tanaka; NK hereafter), has the ability to produce unreduced 2n pollen grains, allowing generation of polyploid progenies via sexual polyploidization. In this study, we developed a method of single-pollen genotyping for citrus and applied it to the analysis of transmission of heterozyg...

    20. A Database of Simple Sequence Repeat (SSR) Marker-Based DNA Profiles of Citrus and Related Cultivars and Germplasm

    文献信息:Hong, J. H., Chae, C. W., Choi, K. J., Kwon, Y. S.等.  发表于【Korean Journal of Horticultural Science & Technology】 | 请登录

    简介:The present study investigated identification of cultivars through phylogenetic analysis of 108 Citrus varieties and related cultivars using simple sequence repeat (SSR) markers. Two hundred three SSR primer pairs were used to detect polymorphic markers among 8 Citrus cultivars consisting of 4 mandarins, 1 orange, 1 tangor, 1 tangelo, and 1 pumelo. Eighteen SSR primer pairs were reproducible and s...

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