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  • 21. Enzymatic depletion of transposable elements in sequencing libraries and its application for genotyping multiplexed CRISPR-edited plants

    文献信息:Thomson, G., Mermaz, B., Sagawa, C. H. D., Lin, C. Y. I., Tachev, M., Joly, V., Irish, V. F. and Jacob, Y.等.  请登录

    简介: Whole-genome sequencing has become a common strategy to genotype individual plants of interest. Although a limited number of genomic regions usually need to be surveyed with this strategy, excess sequencing information is almost always generated at an appreciable financial cost. Repetitive sequences (e.g., transposons), which can account for more than 80% of the genome of some plants, are often n...

    22. CsbHLH6 positively regulates abscisic acid-mediated citrus fruit coloration during postharvest storage

    文献信息:Sun, Quan, He, Zhengchen, Zhang, Yingzi, Feng, Di, Wei, Ranran, Chai, Lijun, Guo, Wenwu, Xu, Juan, Cheng, Yunjiang, Xu, Qiang, Ye, Junli and Deng, Xiu等.  请登录

    简介:As is well known, postharvest abscisic acid (ABA) treatment of citrus fruits can significantly enhance fruit coloration by promoting the accumulation of carotenoids, however, the underlying molecular mechanism is still unclear. In this study, we further confirmed the effect of ABA treatment in promoting citrus fruit coloration by activating the expression of 4 key carotenoid metabolism genes (CsPS...

    23. Genome-wide identification of ARID-HMG related genes in citrus and functional analysis of FhARID1 in apomixis and axillary bud development

    文献信息:Song, X. T., Zhou, Y., Cao, Z., Wang, N., Tian, X. Y., Chai, L. J., Xie, Z. Z., Ye, J. L. and Deng, X. X.等.  请登录

    简介: Polyembryony has posed a significant impediment to the advancement of citrus hybrid breeding. FhRWP is widely regarded as a pivotal factor governing asexual reproduction in citrus, and prior research has demonstrated that FhARID1, acting as an upstream regulator, modulates FhRWP expression. In this study, we performed a genome-wide characterization of the ARID-HMG-related genes using the short ju...

    24. 'US-Gallasch': A New Citrus Rootstock 60 Years in the Making

    文献信息:Smith, M. W., Gultzow, D. L., Newman, T. K., Reid, M., Krueger, R. R., Roose, M. L., Federici, C. T. and Bowman, K. D.等.  请登录

    25. Molecular Characterization of Citrus Accessions Grown for Pre-Breeding Purposes

    文献信息:Soares, I. F. G., Paula, F. C., Oliveira, C. D. B., Neto, J. D. D., Santos, T. D., de Almeida, R. N., Berilli, Apcg, Berilli, S. D., Soares, T. C. B.,等.  请登录

    简介:The objective of this work was to analyse the genetic diversity of a population of Citrus spp. in the south of the State of Esp & iacute;rito Santo, Brazil, for pre-breeding studies. For that, a total of sixty genotypes were analysed, including ten citrus varieties from four species of the Citrus genus. The methodology involved DNA extraction, amplification via polymerase chain reaction, and the u...

    26. The CtrCBL1/CtrCIPK6 Complex of Citrus Phosphorylates CtrBBX32 to Regulate CtrSTP1-Mediated Sugar Accumulation and Cold Tolerance

    文献信息: Shang, X. M., Zhao, Z. Q., Xiao, W., Zeng, Y. K., Li, M. D., Jiang, X., Dahro, B., Chu, L. L., Wang, M., Li, C. L. and Liu, J. H.等.  请登录

    简介:Sugar accumulation is a crucial adaptive strategy for plant cold tolerance, yet its regulatory mechanisms in the roots under cold conditions remain largely unclear. Here, a cold-inducible sugar transporter protein (CtrSTP1), which is highly expressed in the roots of trifoliate orange (Citrus trifoliata L.), is identified. CtrSTP1 functions positively in cold tolerance by facilitating sugar accumul...

    27. The CsMYB36-CsSWEET17 module mediates the calcium-induced sucrose accumulation in citrus

    文献信息: Sheng, X. W., Li, M. D., Luo, Y. R., Mao, Z. L., Zhai, X. W., Liu, J. H. and Li, C. L.等.  请登录

    简介:Sugar content serves as a crucial determinant of fruit flavor quality and nutritional value. Calcium plays extensive regulatory roles in fruit development and quality formation, yet the molecular mechanisms underlying calcium-mediated sugar accumulation remain poorly understood. In this study, we demonstrate that calcium treatment enhances sugar accumulation in both citrus fruits and calli, concom...

    28. Deep resequencing unveils novel SNPs, InDels, and large structural variants for the clonal fingerprinting of sweet orange Citrus sinensis (L.) Osbeck

    文献信息:Scaglione, D., Ciacciulli, A., Gattolin, S., Caruso, M., Marroni, F., Casas, G. L., Jurman, I., Licciardello, G., Catara, A. F., Rossini, L., Licciard等.  请登录

    简介:The large phenotypic variability characterizing the sweet orange [Citrus sinensis (L.) Osbeck] germplasm arose from spontaneous somatic mutations and led to the diversification of major groups (common, acidless, Navel, and pigmented). Substantial divergence also occurred within each varietal group. The genetic basis of such variability (i.e., ripening time, fruit shape, color, acidity, and sugar c...

    29. NPR1 suppresses Candidatus Liberibacter asiaticus-induced callose and reactive oxygen species accumulation

    文献信息: Sarkar, P., El-Mohtar, C., Wang, C., Turner, D., Welker, S., Robertson, C. J., Orbović, V., Mou, Z. and Levy, A.等.  请登录

    简介:Huanglongbing (HLB), a devastating citrus disease caused by Candidatus Liberibacter asiaticus (CLas), triggers persistent immune activation marked by excessive callose deposition and reactive oxygen species (ROS) accumulation, which impairs phloem function. This maladaptive response has led to HLB being described as a 'pathogen-triggered immune disease'. Overexpression of the Arabidopsis (Arabidop...

    30. Impact of Gamma-Irradiated Pollen on Sexual Compatibility, Fruit Quality and Seed Development in Grapefruit Varieties

    文献信息: Rana, M. A., Usman, M., Fatima, B., Rana, I. A. and Rashid, B.等.  请登录

    简介:Grapefruit is a leading citrus fruit crop due to its rich nutraceutical profile. Most of the commercial grapefruit varieties are budsports and have low genetic variability highlighting the need to induce variability in existing germplasm. Pollination using irradiated pollen is an effective method to induce variability and alter fruit quality attributes. Seed parents of grapefruit varieties Shamber...

    31. Transcriptional regulation of thorn tip sclerification in plants

    文献信息:Ren, J., Duan, Y. Y., Li, R. Q., Zhang, X., Shi, Y. F., Zhou, S. Y., Xie, K. D., Wu, X. M., Irish, V. F., Deng, X. X., Zhang, F. and Guo, W. W.等.  请登录

    简介: A common characteristic of thorns, prickles, and spines is a hardened and sharp tip. This feature complicates cultivation and postharvest processing for many crops, yet the molecular mechanisms governing this specific sclerification process remain unclear. By genome editing screening, we identified a MYB family gene named SHORT and SOFT THORN 1 (SST1) that specifically promotes the development of...

    32. WRKY27-SPDS1 module of Ichang papeda (Citrus ichangensis) promotes cold tolerance by modulating spermidine content

    文献信息:Qu, J., Xiao, P., Wang, Y. L., Wang, Y., Xiao, W., Zhang, Y., Xu, X. Y. and Liu, J. H.等.  请登录

    简介:Spermidine (Spd) is one of the predominant polyamines in higher plants and plays a crucial role in combating various abiotic stresses. However, the molecular functions and underlying regulatory mechanisms associated with plant Spd synthase (SPDS) genes in cold tolerance remain poorly understood. In this study, cold treatment markedly induced Spd accumulation and enhanced SPDS activity in Ichang pa...

    33. CsICS gene overexpressed in sweet orange enhanced the resistance to Candidatus Liberibacter asiaticus

    文献信息:Rampim, B. T., Della Coletta, H., Freitas-Astua, J. and Boscariol-Camargo, R. L.等.  请登录

    简介:Huanglongbing (HLB) is a severe disease affecting citrus worldwide, caused by Candidatus Liberibacter asiaticus (CLas). Transgenic plants overexpressing genes that confer resistance to CLas could serve as an effective strategy for controlling HLB. Transgenic citrus plants were previously developed to overexpress the Citrus sinensis (CsICS) (isochorismate synthase) gene, which is involved in salicy...

    34. Heat stress increases mutation efficiency mediated by CRISPR/Cas9 in citrus

    文献信息: Peng, A. H., Chen, Z. Y., Zhu, Y. L., Ye, Z. T., Zou, X. P., He, Y. R., Li, Q., Cao, L. and Chen, S. C.等.  请登录

    简介:The CRISPR/Cas9 system has shown great promise in engineering targeted mutations in a genome. The efficiency of Cas9-mediated genome editing is temperature sensitive. A high-temperature regime can increase the mutation efficiency induced by the CRISPR/Cas9 system in many plant species. However, a heat stress treatment has not been applied during the tissue culture process in citrus. To develop an ...

    35. Nodal culture for efficient regeneration and CRISPR/Cas-based genome editing in recalcitrant horticultural crops

    文献信息:Pandey, H., Misra, V., Sharma, A., Chatterjee, B., Sutradhar, M., Kumar, R., Heisnam, P., Devadas, V. S., Mall, A. K., Tehri, N. and Vashishth, A.等.  请登录

    简介:Nodal culture is a powerful plant tissue culture technique addressing critical challenges such as desiccation, microbial contamination, and the limited viability of explants, particularly in recalcitrant horticultural crops like Garcinia mangostana, Artocarpus heterophyllus, Cucumis melo, Citrus limon, Kinnow mandarin, and Coffea arabica. This method utilizes sterilized immature nodal explants, wi...

    36. Two Caffeoyl-CoA O-methyltransferase-like enzyme are involved in the biosynthesis of polymethoxyflavones in Citrus reticulata 'Chachiensis'

    文献信息:Pan, H. M., Su, J. M., Bai, M., Chen, M. Z., He, Z. Y., Rong, N., Lin, X. T., Wang, H. Y. and Wu, H.等.  请登录

    简介:Polymethoxyflavones (PMFs) are abundant in citrus plants and exhibit remarkable biological activities in the inhibition of the occurrence and development of various tumours. O-methyltransferase (OMTs)-mediated methylation plays a crucial role in the biosynthesis of PMFs. However, the OMTs catalyzing the methylation of hydroxyflavonoids in citrus have not been fully identified. In this study, we id...

    37. Image dataset: UAV images and ground data of one 'Bingo' mandarin and two 'Valencia' orange rootstock trials conducted in Florida

    文献信息:Niedz, R. P. and Bowman, K. D.等.  请登录

    简介:The data are aerial images and ground tree measurement data of 3 citrus rootstock trials. Developing new citrus rootstock varieties requires field trials to test to identify selections with improved horticultural performance. A bud from a scion variety is grafted onto the rootstock and grown in a nursery until the grafted plant is ready to be planted in the field, which is in about one year. Trees...

    38. Low-density SNP marker sets for genetic variation analysis and variety identification in cultivated citrus

    文献信息: Nguyen, P. L., Jung, J. K., Park, J. S. and Sim, S. C.等.  请登录

    简介: BackgroundThe Citrus species are major fruit crops cultivated in the world and have complex genetic relationships due to sexual comparability between Citrus and related genera. Of these, satsuma mandarin (C. unshiu (Mak.) Marc.) and sweet orange (C. sinensis (L.) Osb.) are widely grown diploid species. In this study, genotyping by sequencing (GBS) was conducted to identify single nucleotide polym...

    39. Evaluation of pre-selected mandarin hybrids based on tolerance to alternaria brow spot, physicochemical characteristics and acceptability

    文献信息:Moura, V. S., Verruma-Bernardi, M. R., Rodrigues, J., Cristofani-Yaly, M., de Azevedo, F. A. and Bastianel, M.等.  请登录

    简介:The selection of new mandarin varieties to enhance fruit supply and address phytosanitary challenges is a significant issue in table citrus farming. In Brazil, the primary harvest season for commercial varieties spans from May to August, leaving a gap in the availability of mandarins during the off-season, which typically coincides with the hottest months of the year. Furthermore, diseases such as...

    40. The GRAS transcription factor PtrPAT1 of Poncirus trifoliata functions in cold tolerance and modulates glycine betaine content by regulating the BADH-like gene

    文献信息:Ming, R. H., Fang, T., Ling, W., Geng, J. J., Qu, J., Zhang, Y., Chen, J. H., Yao, S. C., Li, L. B., Huang, D. and Liu, J. H.等.  请登录

    简介:GRAS, termed after gibberellic acid insensitive (GAI), RGA (repressor of GA1), and SCR (scarecrow), is a plant-specific transcription factor crucial for plant development and stress response. However, understanding of the functions played by the GRAS members and their target genes in citrus is limited. In this study, we identified a cold stress-responsive GRAS gene from Poncirus trifoliata, design...

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