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  • 21. Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in Citrus sinensis

    文献信息: Song, Shipeng, Liu, Hui, Miao, Luke, He, Li, Xie, Wenzhao, Lan, Hong, Yu, Changxiu, Yan, Wenkai, Wu, Yufeng, Wen, Xiao-Peng, Xu, Qiang, Deng, Xiuxin 等.  请登录

    简介:Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybri...

    22. Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation

    文献信息:Su, H., Wang, Y., Xu, J., Omar, A. A., Grosser, J. W., Calovic, M., Zhang, L., Feng, Y., Vakulskas, C. A. and Wang, N.等.  请登录

    简介:Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease worldwide. Generating disease-resistant cultivars is the most effective, environmentally friendly and economic approach for disease control. However, citrus traditional breeding is lengthy and laborious. Here, we develop transgene-free canker-resistant Citrus sinensis lines in the T0 generation within 10 m...

    23. A self-regulated transcription factor CsWRKY33 enhances resistance of citrus fruit to Penicillium digitatum

    文献信息:Wang, Wenjun, Li, Ting, Chen, Jialin, Zhang, Xuan, Wei, Linli, Yao, Shixiang and Zeng, Kaifang等.  请登录

    简介:Green mold caused by Penicillium digitatum is a major postharvest disease in citrus fruit, but the molecular mechanism related to the induced disease resistance in citrus is still unclear. This study investigated the regu-latory function of CsWRKY33 on citrus disease resistance. The results showed that CsWRKY33 was located in the nucleus, and the transient overexpression of CsWRKY33 significantly ...

    24. Transcriptional regulatory mechanism of a variant transcription factor CsWRKY23 in citrus fruit resistance to Penicillium digitatum

    文献信息:Wang, Wenjun, Li, Ting, Chen, Qi, Yao, Shixiang and Zeng, Kaifang等.  请登录

    简介: As the most productive fruit in the world, there is a lack of research on disease resistance mechanisms in citrus. WRKY transcription factors are mediators of disease resistance in plants. In this research, CsWRKY23, which could respond to exogenous salicylic acid (SA) was identified. As a variant WRKY, the transient overexpression of CsWRKY23 in citrus peel enhanced the resistance to Penicillium...

    25. A citrus pulp mastication trait-related ABSCISIC ACID-INSENSITIVE 5-like protein promotes fruit softening via transcriptionally activating pectate lyase gene

    文献信息:Wang, Xun, Li, Yuan, Lu, Wen, Zhang, Mingfei, Deng, Honghong, Xiong, Bo, Liao, Ling, Tang, Yi, Lin, Lijin, Zhao, Junming and Wang, Zhihui Year: 2023等.  请登录

    简介:Mastication trait of citrus fruit is an important criterion to evaluate mandarin citrus fruit quality and decides its market value. However, the regulatory mechanism underlying the mastication trait formation is unclear. Here we isolated a bZIP family member, annotated to be "ABSCISIC ACID-INSENSITIVE 5-like protein 5", from fruits of two mandarin citrus cultivars with different mastication trait,...

    26. Genome-wide identification of the CsPAL gene family and functional analysis for strengthening green mold resistance in citrus fruit

    文献信息:Wei, Linli, Wang, Wenjun, Li, Ting, Chen, Ou, Yao, Shixiang, Deng, Lili and Zeng, Kaifang等.  请登录

    简介:Phenylalanine aminolyase (PAL) is a key rate-limiting enzyme. It catalyzes the initial step of the phenylpropane metabolic pathway. PAL is involved in the regulation of fruit diseases, but the specific function of the PAL gene in the regulation of postharvest fruit diseases is unknown. In this study, we identified four CsPAL members in citrus, including CsPAL1-CsPAL4. Based on physicochemical prop...

    27. The functional analysis of sugar transporter proteins in sugar accumulation and pollen tube growth in pummelo (Citrus grandis)

    文献信息:Xu, Weiwei, Liu, Ziyan, Zhao, Zeqi, Zhang, Shuhang, Li, Mengdi, Guo, Dayong, Liu, Ji-Hong and Li, Chunlong等.  请登录

    简介:Sugar transporter proteins (STPs) play vital roles in sugar transport and allocation of carbon sources in plants. However, the evolutionary dynamics of this important gene family and their functions are still largely unknown in citrus, which is the largest fruit crop in the world. In this study, fourteen non-redundant CgSTP family members were identified in pummelo (Citrus grandis). A comprehensiv...

    28. CsERF003, CsMYB7 and CsMYB102 promote cuticular wax accumulation by upregulating CsKCS2 at fruit ripening in Citrus sinensis

    文献信息:Yang, Hongbin, Zhang, Mingfei, Li, Xin, Zhu, Zhifeng, Xu, Rangwei, Zhu, Feng, Xu, Juan, Deng, Xiuxin and Cheng, Yunjiang等.  请登录

    简介: Cuticular wax is a hydrophobic protective layer covering the surface of citrus fruit, which plays an important role in preventing fruit water loss and improving fruit appearance quality. Our previous study has demonstrated that CsKCS2 is highly expressed at fruit ripening and involved in fruit cuticular wax synthesis in Citrus sinensis. However, the upstream regulators of CsKCS2 and the transcrip...

    29. A bZIP transcription factor (CiFD) regulates drought- and low-temperature-induced flowering by alternative splicing in citrus

    文献信息:Ye, Li-Xia, Wu, Yan-Mei, Zhang, Jin-Xia, Zhang, Jin-Xin, Zhou, Huan, Zeng, Ren-Fang, Zheng, Wei-Xuan, Qiu, Mei-Qi, Zhou, Jing-Jing, Xie, Zong-Zhou, Hu等.  请登录

    简介:Drought and low temperature are two key environmental factors that induce adult citrus flowering. However, the underlying regulation mechanism is poorly understood. The bZIP transcription factor FD is a key component of the florigen activation complex (FAC) which is composed of FLOWERING LOCUS T (FT), FD, and 14-3-3 proteins. In this study, isolation and characterization of CiFD in citrus found th...

    30. Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of beta-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)

    文献信息:Yue, Pengtao, Jiang, Zhenghua, Sun, Quan, Wei, Ranran, Yin, Yingzi, Xie, Zongzhou, Larkin, Robert M., Ye, Junli, Chai, Lijun and Deng, Xiuxin等.  请登录

    简介:Carotenoids are natural pigments that influence the color of citrus fruit. The red-colored carotenoid beta-citraurin is responsible for the peel color in "Newhall" orange (Citrus sinensis). Although jasmonates are known to regulate the biosynthesis and accumulation of carotenoids, their effects on beta-citraurin biosynthesis in citrus fruit remain unclear. Here, we determined that treatment with m...

    31. CcRR5 interacts with CcRR14 and CcSnRK2s to regulate the root development in citrus

    文献信息:Zhang, M. M., Wang, F. S., Hu, Z., Wang, X. L., Yi, Q., Feng, J. P., Zhao, X. C. and Zhu, S. P.等.  请登录

    简介: Response regulator (RR) is an important component of the cytokinin (CK) signal transduction system associated with root development and stress resistance in model plants. However, the function of RR gene and the molecular mechanism on regulating the root development in woody plants such as citrus remain unclear. Here, we demonstrate that CcRR5, a member of the type A RR, regulates the morphogenes...

    32. Citrus beta-carotene hydroxylase 2 (BCH2) participates in xanthophyll synthesis by catalyzing the hydroxylation of beta-carotene and compensates for BCH1 in citrus carotenoid metabolism

    文献信息:Zhang, Yingzi, Jin, Jiajing, Zhu, Shenchao, Sun, Quan, Zhang, Yin, Xie, Zongzhou, Ye, Junli and Deng, Xiuxin等.  请登录

    简介: As an essential horticultural crop, Citrus has carotenoid diversity, which affects its aesthetic and nutritional values. beta,beta-Xanthophylls are the primary carotenoids accumulated in citrus fruits, and non-heme di-iron carotene hydroxylase (BCH) enzymes are mainly responsible for beta,beta-xanthophyll synthesis. Previous studies have focused on the hydroxylation of BCH1, but the role of its p...

    33. Host-derived chimeric peptides clear the causative bacteria and augment host innate immunity during infection: A case study of HLB in citrus and fire blight in apple

    文献信息:Basu, Supratim, Sineva, Elena, Nguyen, Liza, Sikdar, Narattam, Park, Jong Won, Sinev, Mikhail, Kunta, Madhurababu and Gupta, Goutam等.  请登录

    简介: Bacterial diseases cause severe losses in the production and revenue of many fruit crops, including citrus and apple. Huanglongbing (HLB) in citrus and fire blight in apple are two deadly diseases without any cure. In this article, we introduce a novel therapy for HLB and fire blight by enhancing the innate immunity of the host plants. Specifically, we constructed in silico a library of chimeras ...

    34. Characterization of three tandem-duplicated calcium binding protein (CaBP) genes and promoters reveals their roles in the phytohormone and wounding responses in citrus

    文献信息:Bei, Xuejun, Wang, Shaohua, Huang, Xia, Zhang, Xiuli, Zhou, Jiayi, Zhang, Huiting, Li, Guoguo and Cheng, Chunzhen等.  请登录

    简介:Accumulated evidences have revealed the critical roles of calcium binding protein (CaBP) in growth and stress responses of plants. However, its function in woody plants is poorly understood. In this study, we cloned the CDS, gDNA and promoter sequences of three tandem-duplicated CaBPs (CsCaBP1, CsCaBP2 and CsCaBP3) from Citrus sinensis, analyzed their sequence characteristics, and investigated the...

    35. Reproductive Biology Factors Hampering Lemon Citrus limon (L.) Burm. f. Genetic Improvement

    文献信息:Catalano, Chiara, Casas, Giuseppina Las, Giuffrida, Alessio, Ferlito, Filippo, Di Guardo, Mario, Continella, Alberto, Bennici, Stefania, La Malfa, Ste等.  请登录

    简介:Background: Floral sterility and nucellar embryony are peculiar traits of several Citrus species and represent an obstacle to traditional breeding. Morphological sterility mainly results in pistil abortion and anther atrophy, while polyembryony is due to a mechanism known as sporophytic apomixis, which consists of the presence of embryos in the seed generated from the nucellar (maternal) tissue al...

    36. Characterization of copper-induced-release of exudates by Citrus sinensis roots and their possible roles in copper-tolerance

    文献信息:Chen, Huan-Huan, Chen, Xu-Feng, Zheng, Zhi-Chao, Huang, Wei-Lin, Guo, Jiuxin, Yang, Lin-Tong and Chen, Li Song等.  请登录

    简介:Copper (Cu) excess is often observed in old Citrus orchards. Little information is available on the characterization of Cu-induced-release of root exudates and their possible roles in plant Cu-tolerance. Using sweet orange [Citrus sinensis (L.) Osbeck cv. Xuegan] seedlings as materials, we investigated the impacts of 0, 0.5, 25, 150, 350, 550, 1000, 2000 or 5000 mu M CuCl2 (pH 4.8) on Cu uptake, r...

    37. The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection

    文献信息:Cheng, Chunzhen, Zhong, Yun, Wang, Bin, Zhang, Yongyan, Wu, Huan, Jiang, Nonghui, Wu, Bo, Lv, Yuanda and Jiang, Bo等.  请登录

    简介:The citrus industry has been threatened by Huanglongbing (HLB) for over a century. Here, an HLB-induced Arabidopsis RPM1-interacting protein 4 (RIN4) homologous gene was cloned from Citrus clementina, and its characteristics and function were analyzed to determine its role during citrus-Candidatus Liberibacter asiaticus (CLas) interactions. Quantitative real-time PCR showed that RIN4 was expressed...

    38. Increasing the production of limonin 17-beta-D-glucopyranoside in hairy roots of Citrus x paradisi. cv Duncan by overexpression of limonoid UDP-glucosyltransferase via Agrobacterium rhizogenes mediat

    文献信息:Cheraei, Solmaz, Eskandari, Forough, Yadollahi, Abbas and Zaare-Nahandi, Fariborz Year: 2022等.  请登录

    简介:Limonin 17-beta-D-glucopyranoside (LG) is a terpenoid compound with a high medicinal value produced in different Citrus species. This substance has high anti-cancer and antioxidant properties and protects the liver from damage. Although LG is tasteless, many compounds related to it are very bitter, although these bitter substances also have significant medicinal properties. Usually, in most Citrus...

    39. Disease resistance induction by K. marxianus against green mold in citrus via transcriptome analysis and verification based on metabolites

    文献信息:Delali, Kottoh Isaac, Chen, Ou, Wang, Wenjun and Zeng, Kaifang等.  请登录

    简介: This study examined the transcriptional composition and changes in gene expression patterns in citrus fruit tissue treated with Kluyveromyces marxianus and its effects on key defense-related enzyme activities and metabolites. This analysis was carried out by the technique of RNA sequencing, the use of HPLC-MS, and GC-MS equipment. The sequencing data revealed that the induction of K. marxianus in...

    40. Overexpression of a "Candidatus Liberibacter Asiaticus" Effector Gene CaLasSDE115 Contributes to Early Colonization in Citrus sinensis

    文献信息: Du, M., Wang, S., Dong, L., Qu, R., Zheng, L., He, Y., Chen, S. and Zou, X.等.  2022-03-11 请登录

    简介:Huanglongbing (HLB), caused by "Candidatus liberibacter asiaticus" (CaLas), is one of the most devastating diseases in citrus but its pathogenesis remains poorly understood. Here, we reported the role of the CaLasSDE115 (CLIBASIA_05115) effector, encoded by CaLas, during pathogen-host interactions. Bioinformatics analyses showed that CaLasSDE115 was 100% conserved in all reported CaLas strains but...

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