标题:Regulatory cis-elements on citrus peel-specific expressed gene, CuCRTISO-like, promoter respond to hormones and abiotic stresses in transgenic Arabidopsis

作者:Eun, Chang-Ho Kim, Seong- U. Kim, In-Jung

作者单位:Faculty of Biotechnology, College of Applied Life Sciences and Research Institute for Subtropical Agriculture and Biotechnology, SARI, Jeju National University, Jeju 63243, Republic of&am

摘要:We identified a peel-specific expressed gene in Citrus unshiu fruits by differentially expressed gene (DEG) analysis, which showed a homology with carotenoid isomerase-like genes identified from other plants and, therefore, designated as CuCRTISO-like. Here we determined the promoter sequence of CuCRTISO-like and analyzed histochemical GUS activity using transgenic Arabidopsis plants harboring CuCRTISO-like promoter-GUS gene constructs (pCRTL-Prom1 similar to pCRTL-Prom5 lines). The promoter activity of CuCRTISO-like was detected in the cotyledon at 5 and 10 days after germination (DAG), young leaf, and anther, but not in the cotyledon at 15 DAG and mature leaf. Several cis-acting elements involved in hormones and abiotic stresses are located on the CuCR-TISO- like promoter. Salicylic acid and ethylene treatments induced the GUS activity in the pCRTL-prom1 and pCRTL-Prom4 line, respectively. Treatment of drought and wounding stress induced the GUS activity in the pCRTL-Prom4 and pCRTL-Prom3 line, respectively. Heat stress treatment induced GUS activity more strongly as the promoter length decreased except for no GUS activity in the pCRTL-Prom5 line. The CuCRTISO-like expression during fruit maturation of C. unshiu showed a peel-specific expression pattern. Our results suggest that CuCRTISO-like promoter activity is regulated in a developmental and organ-specific manner, and responds to hormones and abiotic stresses.

关键词:Peel-specific expressed gene ,Cis-acting element ,CuCRTISO-like , GUS activity ,

文献注录:Eun, Chang-Ho Kim, Seong- U. Kim, In-Jung. Regulatory cis-elements on citrus peel-specific expressed gene, CuCRTISO-like, promoter respond to hormones and abiotic stresses in transgenic Arabidopsis [J]. Plant Biotechnology Reports. 2017, 1: 63-69.

报/刊名:Plant Biotechnology Reports》,发表于2017 / 第 1 期。

文献类型: [J] (文献级别:SCI刊物

页码: 63-69 页 / 共7

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