标题:Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing

作者:Reyes, C. A., De Francesco, A., Ocolotobiche, E. E., Costa, N., Garcia, M. L.

作者单位:(M.L. García) Instituto de Biotecnología y Biología Molecular, CCT-La Plata, CONICET - UNLP, calles 47 y 115 (1900), La Plata, Buenos Aires, Argentina Email:garcia_m@biol.unlp.edu.ar

摘要:Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the recovery observed in non-transgenic plants. Ihp24K lines did not behave similarly when challenged with Citrus tristeza virus. All these results indicate that hypersusceptibility is likely related to the specific action of 24K-derived hairpin over CPsV multiplication.

关键词:Citrus psorosis virus,Hairpin-RNA, Transgenic sweet orange, Hypersusceptibility

文献注录:Reyes, C. A., De Francesco, A., Ocolotobiche, E. E., Costa, N., Garcia, M. L.. Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing [J]. Physiological and Molecular Plant Pathology. 2016, 94: 149-155.

报/刊名:Physiological and Molecular Plant Pathology》,发表于2016 / 第 94 期。

文献类型: [J]

页码: 149-155 页 / 共7

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