作者：Hong, J. H.， Chae, C. W.， Choi, K. J.， Kwon, Y. S.
作者单位：[Hong, Jee-Hwa] Minist Agr Food & Rural Affairs, Natl Agr Prod Qual Management Serv, Expt Res Inst, Gimcheon 39660, South Korea. [Chae, Chi-Won] Minist Agr Food & Rural Affairs, Korea Seed & Variety Serv, Jeju Off, Seogwipo 63613, South K
摘要：The present study investigated identification of cultivars through phylogenetic analysis of 108 Citrus varieties and related cultivars using simple sequence repeat (SSR) markers. Two hundred three SSR primer pairs were used to detect polymorphic markers among 8 Citrus cultivars consisting of 4 mandarins, 1 orange, 1 tangor, 1 tangelo, and 1 pumelo. Eighteen SSR primer pairs were reproducible and showed highly polymorphic alleles. These markers were applied to assess genetic variations of the 108 varieties. Each marker detected 5-14 alleles, with an average of 9.28. The polymorphism information content varied from 0.417 to 0.791 with an average of 0.706. Cluster analysis with SSR markers resulted in 13 major groups reflecting cultivar types and pedigree inforniation. Twelve orange cultivars in the I-1(st) sub-cluster and 23 mandarin cultivars in the II-1(st) sub-cluster, respectively, were not discriminated using the SSR markers. This could be due to narrow genetic backgrounds originated through bud mutation or nucellars seedlings. The SSR profile database of Citrus cultivars will be useful as a tool for protection of plant breeders\' intellectual property rights in addition to assessing genetic diversity in Citrus cultivars and germplasms.
关键词：alleles , buds , cultivars , DNA , genetic diversity , genetic markers , genetic
文献注录：Hong, J. H.， Chae, C. W.， Choi, K. J.， Kwon, Y. S.. A Database of Simple Sequence Repeat (SSR) Marker-Based DNA Profiles of Citrus and Related Cultivars and Germplasm [J]. Korean Journal of Horticultural Science & Technology. 2016, 34(1): 142-153.
报/刊名：《Korean Journal of Horticultural Science & Technology》，发表于2016 / 第 34(1) 期。
文献类型： [J]
页码：第 142-153 页 / 共12 页